Localization of ?1-microglobulin (HC protein) in normal human tissues: An immunohistochemical study using monoclonal antibodies

1984 ◽  
Vol 16 (12) ◽  
pp. 1311-1324 ◽  
Author(s):  
Patrick Bouic ◽  
Claude Vincent ◽  
Jean-Pierre Revillard
Keyword(s):  
Monoclonal Antibodies ◽  
Immunohistochemical Study ◽  
Human Tissues ◽  
Normal Human

Protein phosphatase 2Cα expression in normal human tissues: an immunohistochemical study

2001 ◽  
Vol 116 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Beatriz Lifschitz-Mercer ◽  
Yuri Sheinin ◽  
Daniella Ben-Meir ◽  
Letizia Bramante-Schreiber ◽  
Leonor Leider-Trejo ◽  
...  
Keyword(s):  
Protein Phosphatase ◽  
Immunohistochemical Study ◽  
Human Tissues ◽  
Normal Human

Immunohistochemical study of angiotensin-converting enzyme in human tissues using monoclonal antibodies

1987 ◽  
Vol 87 (5) ◽  
pp. 487-490 ◽  
Author(s):  
S. M. Danilov ◽  
A. I. Faerman ◽  
O. Yu. Printseva ◽  
A. V. Martynov ◽  
I. Yu. Sakharov ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Angiotensin Converting Enzyme ◽  
Immunohistochemical Study ◽  
Human Tissues ◽  
Converting Enzyme

scholarly journals Determination of platelet antigens and glycoproteins in the human fetus

Blood ◽  
1986 ◽  
Vol 68 (2) ◽  
pp. 488-492 ◽  
Author(s):  
Y Gruel ◽  
B Boizard ◽  
F Daffos ◽  
F Forestier ◽  
J Caen ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Fetus ◽  
Antenatal Diagnosis ◽  
Polyclonal Antibodies ◽  
Umbilical Vein ◽  
Membrane Surface ◽  
Platelet Suspension ◽  
Direct Puncture ◽  
Normal Human

Abstract The autosomal recessive transmission of Glanzmann's thrombasthenia (GT) and Bernard-Soulier syndrome (BSS), together with requests of families who already had children with these diseases, prompted us to investigate the feasibility of their antenatal diagnosis. The preliminary step leading to the early detection of GT or BSS was to characterize, in the normal human fetus, the platelet antigens and glycoproteins (GPs) and to define their normal amounts on the membrane surface. Blood samples from 32 fetuses between 18 to 26 weeks of gestation were collected by direct puncture of the umbilical vein using an ultrasound-guided needle. Polyclonal antibodies from human origin directed against PLA1, Leka antigens, and the GPIIb IIIa complex (IgGL), or murine monoclonal antibodies specific for GPIb (AN51, 6D1), GPIIIa (AP-3), or GPIIb IIIa (AP-2) were studied using platelet suspension immunofluorescence tests. The binding of each antibody was quantified using a cytofluorograph (Ortho 50H). PLA1 and Leka antigens were expressed in normal amounts on fetal platelets as early as 16 weeks of intrauterine life. The GPIIb IIIa complex quantified by polyclonal or monoclonal antibodies was in the same range in fetuses (IgGL = 427 +/- 23 AUF, AP-2 = 459.5 +/- 8.5; AP-3 = 536 +/- 14) and in adults (IgGL = 420 +/- 30; AP-2 = 498 +/- 11; AP-3 = 515 +/- 13). The platelet binding of antibodies that recognized GPIb was higher in fetuses (AN51 = 491.5 +/- 14; 6D1 = 479 +/- 15) than in adults (AN51 = 426.5 +/- 9; 6D1 = 449 +/- 8.7). These results suggest that immunological techniques can be applied as early as 18 weeks of gestation for the antenatal diagnosis of GT and BSS.

Euonymus europaeus lectin as an endothelial and epithelial marker in canine tissues

1992 ◽  
Vol 26 (2) ◽  
pp. 114-121 ◽  
Author(s):  
F. Roussell ◽  
J. Dalion ◽  
J. C. Wissocq
Keyword(s):  
Endothelial Cells ◽  
Monoclonal Antibodies ◽  
Binding Site ◽  
Binding Sites ◽  
Human Tissues ◽  
Probable Structure ◽  
Euonymus Europaeus ◽  
Epithelial Marker ◽  
Endothelial Marker

The Euonymus europaeus agglutinin (EEA) is an endothelial marker in mammalia. In canine tissues, 4 types of endothelial cells (general, nervous, arterial, hepatic) were identified by the presence of the EEA receptor and by its sensitivity to neuraminidase enhancement. In adult dogs, EEA binding saccharides had endothelial or epithelial distributions and reactivities similar to those described for human tissues. Different EEA reactivities were observed between fetal, neonatal and adult canine tissues mainly at the arterial level. These findings suggest that the development of the binding sites is not identical in dog and man. Related lectins and monoclonal antibodies were used to characterize the EEA binding site, and the probable structure of the EEA binding saccharide in endothelial cells appeared to be αGal (1,3) βGal (1,4) GIcNAc.

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