Mode of action on the platelet 5-HT of the active principles in Toh's alkaline tissue extract

H. Shio

doi:10.1007/bf00999395

Mode of action on the platelet 5-HT of the active principles in Toh's alkaline tissue extract

Naunyn-Schmiedeberg s Archives of Pharmacology ◽

10.1007/bf00999395 ◽

1970 ◽

Vol 267 (2) ◽

pp. 123-134 ◽

Cited By ~ 1

Author(s):

H. Shio

Keyword(s):

Mode Of Action ◽

Tissue Extract ◽

Alkaline Tissue Extract

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Liberation of platelet histamine by the alkaline tissue extract and oleic acid

Cellular and Molecular Life Sciences ◽

10.1007/bf02113010 ◽

1970 ◽

Vol 26 (12) ◽

pp. 1329-1330

Author(s):

K. Kataoka ◽

M. Sorimachi ◽

H. Shio ◽

S. Nagata ◽

A. Inouye

Keyword(s):

Oleic Acid ◽

Tissue Extract ◽

Alkaline Tissue Extract

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Effects of 5-5'-Diphenyl-2-thiohydantoin (DPTH) and Thyroxine on the Ultrastructure and Chemical Composition of Rat Liver

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100066917 ◽

1971 ◽

Vol 29 ◽

pp. 570-571

Author(s):

E. A. Elfont ◽

R. B. Tobin ◽

D. G. Colton ◽

M. A. Mehlman

Keyword(s):

Chemical Composition ◽

Rat Liver ◽

Future Study ◽

Mode Of Action ◽

Liver Mitochondria ◽

Rat Liver Mitochondria ◽

Effective Inhibitor ◽

Biochemical Effects ◽

Glycerophosphate Dehydrogenase ◽

Stimulation Of

Summary5,-5'-diphenyl-2-thiohydantoin (DPTH) is an effective inhibitor of thyroxine (T4) stimulation of α-glycerophosphate dehydrogenase in rat liver mitochondria. Because this finding indicated a possible tool for future study of the mode of action of thyroxine, the ultrastructural and biochemical effects of DPTH and/or thyroxine on rat liver mere investigated.Rats were fed either standard or DPTH (0.06%) diet for 30 days before T4 (250 ug/kg/day) was injected. Injection of T4 occurred daily for 10 days prior to sacrifice. After removal of the liver and kidneys, part of the tissue was frozen at -50°C for later biocheailcal analyses, while the rest was prefixed in buffered 3.5X glutaraldehyde (390 mOs) and post-fixed in buffered 1Z OsO4 (376 mOs). Tissues were embedded in Araldlte 502 and the sections examined in a Zeiss EM 9S.Hepatocytes from hyperthyroid rats (Fig. 2) demonstrated enlarged and more numerous mitochondria than those of controls (Fig. 1). Glycogen was almost totally absent from the cytoplasm of the T4-treated rats.

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