The size heterogeneity of human lysyl oxidase mRNA is due to alternate polyadenylation site and not alternate exon usage

1995 ◽  
Vol 21 (2) ◽  
pp. 95-103 ◽  
Author(s):  
Charles D. Boyd ◽  
Thomas J. Mariani ◽  
Youngho Kim ◽  
Katalin Csiszar
Keyword(s):  
Lysyl Oxidase ◽  
Polyadenylation Site ◽  
Exon Usage ◽  
Size Heterogeneity ◽  
Alternate Polyadenylation

Cell-specific expression of secreted versus membrane forms of immunoglobulin gamma 2b mRNA involves selective use of alternate polyadenylation sites

1985 ◽  
Vol 5 (10) ◽  
pp. 2514-2520
Author(s):  
C Milcarek ◽  
B Hall
Keyword(s):  
Heavy Chain ◽  
Plasma Cells ◽  
Single Gene ◽  
Polyadenylation Site ◽  
B Cell Differentiation ◽  
Specific Expression ◽  
Myeloma Cells ◽  
Isolated Nuclei ◽  
Mrna Polyadenylation ◽  
Alternate Polyadenylation

Immunoglobulin heavy chain genes encode at least two forms of mRNA, secretory- and membrane-specific. In less mature B cells and tumors arising from them, lymphomas, the membrane form of the protein and mRNA are in high abundance, while in more mature stages, plasma cells, and myeloma tumor cells, the secreted forms of protein and mRNA predominate. In myeloma cells producing approximately 8:1 ratios of secretory- to membrane-encoding forms of gamma-heavy chain mRNA, we observed equimolar transcription of the secretory- and membrane-encoding exons of the gene. In isolated nuclei from 4T001 (gamma 2b) and K23 (gamma 2a) myeloma cells, the secretory-encoding mRNA polyadenylation site was used at least three times as often as the membrane-encoding mRNA polyadenylation site. In the A20 (gamma 2a) lymphoma, which produces equal amounts of mature secretory- and membrane-encoding heavy chain mRNAs, results of experiments with isolated nuclei showed that the membrane mRNA polyadenylation site was used about two times as often as the secretory mRNA polyadenylation site. Selective use of alternate polyadenylation and cleavage sites, therefore, can modulate the production of the two mRNAs from a single gene during B cell differentiation.

scholarly journals Cell-specific expression of secreted versus membrane forms of immunoglobulin gamma 2b mRNA involves selective use of alternate polyadenylation sites.

1985 ◽  
Vol 5 (10) ◽  
pp. 2514-2520 ◽  
Author(s):  
C Milcarek ◽  
B Hall
Keyword(s):  
Heavy Chain ◽  
Plasma Cells ◽  
Single Gene ◽  
Polyadenylation Site ◽  
B Cell Differentiation ◽  
Specific Expression ◽  
Myeloma Cells ◽  
Isolated Nuclei ◽  
Mrna Polyadenylation ◽  
Alternate Polyadenylation

Immunoglobulin heavy chain genes encode at least two forms of mRNA, secretory- and membrane-specific. In less mature B cells and tumors arising from them, lymphomas, the membrane form of the protein and mRNA are in high abundance, while in more mature stages, plasma cells, and myeloma tumor cells, the secreted forms of protein and mRNA predominate. In myeloma cells producing approximately 8:1 ratios of secretory- to membrane-encoding forms of gamma-heavy chain mRNA, we observed equimolar transcription of the secretory- and membrane-encoding exons of the gene. In isolated nuclei from 4T001 (gamma 2b) and K23 (gamma 2a) myeloma cells, the secretory-encoding mRNA polyadenylation site was used at least three times as often as the membrane-encoding mRNA polyadenylation site. In the A20 (gamma 2a) lymphoma, which produces equal amounts of mature secretory- and membrane-encoding heavy chain mRNAs, results of experiments with isolated nuclei showed that the membrane mRNA polyadenylation site was used about two times as often as the secretory mRNA polyadenylation site. Selective use of alternate polyadenylation and cleavage sites, therefore, can modulate the production of the two mRNAs from a single gene during B cell differentiation.

scholarly journals Streamlining differential exon and 3’ UTR usage with diffUTR

2021 ◽  
Author(s):  
Stefan Gerber ◽  
Gerhard Schratt ◽  
Pierre-Luc Germain
Keyword(s):  
State Of The Art ◽  
Alternative Polyadenylation ◽  
Real Data ◽  
Polyadenylation Site ◽  
Bioconductor Package ◽  
Transcriptomic Data ◽  
Biological Phenomena ◽  
Exon Usage ◽  
Usage Analysis

AbstractBackgroundDespite the importance of alternative poly-adenylation and 3’ UTR length for a variety of biological phenomena, there are limited means of detecting UTR changes from standard transcriptomic data.ResultsWe present the diffUTR Bioconductor package which streamlines and improves upon differential exon usage (DEU) analyses, and leverages existing DEU tools and alternative polyadenylation site databases to enable differential 3’ UTR usage analysis. We demonstrate the diffUTR features and show that it is more flexible and more accurate than state-of-the-art alternatives, both in simulations and in real data.ConclusionsdiffUTR enables differential 3’ UTR analysis and more generally facilitates DEU and the exploration of their results.

A Novel Method for the Rapid Purification of Human and Rat Fibrin(OGEN) Degradation Products in High Yields

1979 ◽  
Author(s):  
W. Nieuwenhuizen ◽  
I. A. M. van Ruijven-Vermeer ◽  
F. Haverkate ◽  
G. Timan
Keyword(s):  
Degradation Products ◽  
Complete Separation ◽  
Purification Method ◽  
Amino Terminal ◽  
Novel Method ◽  
Rapid Purification ◽  
The One ◽  
High Yields ◽  
Size Heterogeneity ◽  
D Dimer

A novel method will be described for the preparation and purification of fibrin(ogen) degradation products in high yields. The high yields are due to two factors. on the one hand an improved preparation method in which the size heterogeneity of the degradation products D is strongly reduced by plasmin digestion at well-controlled calcium concentrations. At calcium concentrations of 2mM exclusively D fragments, M.W.= 93-000 (Dcate) were formed; in the presence of 1OmM EGTA only fragments M.W.= 80.000 (D EGTA) were formed as described. on the other hand a new purification method, which includes Sephadex G-200 filtration to purify the D:E complexes and separation of the D and E fragments by a 16 hrs. preparative isoelectric focussing. The latter step gives a complete separation of D (fragments) (pH = 6.5) and E fragments (at pH = 4.5) without any overlap, thus allowing a nearly 100% recovery in this step. The overall recoveries are around 75% of the theoretical values. These recoveries are superior to those of existing procedures. Moreover the conditions of this purification procedure are very mild and probably do not affect the native configuration of the products. Amino-terminal amino acids of human Dcate, D EGTA and D-dimer are identical i.e. val, asx and ser. in the ratgly, asx and ser were found. E 1% for rat Dcate=17-8 for rat D EGTA=16.2 and for rat D- dimer=l8.3. for the corresponding human fragments, these values were all 20.0 ± 0.2.

Lysyl Oxidase is essential for hypoxia-induced metastasis in breast cancer

2006 ◽  
Vol 66 (S 01) ◽  
Author(s):  
JT Erler ◽  
N Dornhöfer ◽  
S Jeffrey ◽  
A Giaccia
Keyword(s):  
Breast Cancer ◽  
Lysyl Oxidase
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