The activation of phosphatidylinositol turnover is not directly involved in the modulation of neurotransmitter release mediated by presynaptic muscarinic receptors

1988 ◽  
Vol 13 (9) ◽  
pp. 903-907 ◽  
Author(s):  
Mario Marchi ◽  
Giovanni Fontana ◽  
Paolo Paudice ◽  
Maurizio Raiteri
Keyword(s):  
Muscarinic Receptors ◽  
Neurotransmitter Release ◽  
Phosphatidylinositol Turnover ◽  
Presynaptic Muscarinic Receptors

Roles of M2 and M4 Muscarinic Receptors in Regulating Acetylcholine Release From Myenteric Neurons of Mouse Ileum

2005 ◽  
Vol 93 (5) ◽  
pp. 2841-2848 ◽  
Author(s):  
Tadayoshi Takeuchi ◽  
Kaori Fujinami ◽  
Hiroto Goto ◽  
Akikazu Fujita ◽  
Makoto M. Taketo ◽  
...  
Keyword(s):  
Muscarinic Receptors ◽  
Wild Type Mouse ◽  
Electrical Field Stimulation ◽  
Enteric Neurons ◽  
Wild Type ◽  
Ach Release ◽  
Mouse Small Intestine ◽  
Presynaptic Muscarinic Receptors ◽  
In The Wild ◽  
Mouse Ileum

We investigated the subtype of presynaptic muscarinic receptors associated with inhibition of acetylcholine (ACh) release in the mouse small intestine. We measured endogenous ACh released from longitudinal muscle with myenteric plexus (LMMP) preparations obtained from M1–M5 receptor knockout (KO) mice. Electrical field stimulation (EFS) increased ACh release in all LMMP preparations obtained from M1–M5 receptor single KO mice. The amounts of ACh released in all preparations were equal to that in the wild-type mice. Atropine further increased EFS-induced ACh release in the wild-type mice. Unexpectedly, atropine also increased, to a similar extent, EFS-induced ACh release to the wild-type mice in all M1–M5 receptor single KO mice. In M2 and M4 receptor double KO mice, the amount of EFS-induced ACh release was equivalent to an atropine-evoked level in the wild-type mouse, and further addition of atropine had no effect. M2 receptor immunoreactivity was located in both smooth muscle cells and enteric neurons. M4 receptor immunoreactivity was located in the enteric neurons, being in co-localization with M2 receptor immunoreactivity. These results indicate that both M2 and M4 receptors mediate the muscarinic autoinhibition in ACh release in the LMMP preparation of the mouse ileum, and loss of one of these subtypes can be compensated functionally by a receptor that remained. M1, M3, and M5 receptors do not seem to be involved in this mechanism.

scholarly journals Presynaptic muscarinic receptors mediating inhibition of neurogenic contractions in rabbit vas deferens are of the ganglionic M1-type

1988 ◽  
Vol 158 (3) ◽  
pp. 233-242 ◽  
Author(s):  
Manfrid Eltze ◽  
Gernot Gmelin ◽  
Jürgen Wess ◽  
Carsten Strohmann ◽  
Reinhold Tacke ◽  
...  
Keyword(s):  
Muscarinic Receptors ◽  
Vas Deferens ◽  
Presynaptic Muscarinic Receptors ◽  
Rabbit Vas Deferens ◽  
M1 Type
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