Protein organization of rat synaptic plasma membranes and synaptic vesicles: A one- and two-dimensional study

1986 ◽  
Vol 11 (12) ◽  
pp. 1683-1697 ◽  
Author(s):  
Maurizio Popoli ◽  
Blake W. Moore
Keyword(s):  
Synaptic Vesicles ◽  
Plasma Membranes ◽  
Two Dimensional ◽  
Synaptic Plasma Membranes

scholarly journals Characterization of translation products of the polyadenylated RNA of free and membrane-bound polyribosomes of rat forebrain

1984 ◽  
Vol 219 (3) ◽  
pp. 751-761 ◽  
Author(s):  
C Hall ◽  
L Mahadevan ◽  
S Whatley ◽  
G Biswas ◽  
L Lim
Keyword(s):  
Rat Brain ◽  
Plasma Membranes ◽  
Neuron Specific Enolase ◽  
Two Dimensional ◽  
Synaptic Plasma Membranes ◽  
Two Dimensional Gel Electrophoresis ◽  
Specific Product ◽  
Polyadenylated Rna ◽  
Microsomal Membranes ◽  
Membrane Bound

Poly(A)+ RNA (polyadenylated RNA) isolated from membrane-bound and free polyribosomes was translated in reticulocyte lysates, and the products were analysed by two-dimensional gel electrophoresis. Several translation products were specific to membrane-bound polyribosomal mRNA, including polypeptides of 47kDa, 35kDa and 21 kDa, whereas others (e.g. of 37 kDa, 17 kDa and 14 kDa) were specific to free polyribosomal mRNA. Although many products were common to both mRNA species, cross-contamination could be ruled out on the basis of the presence of these and other specific products. The common products included a 68 kDa microtubule-associated protein, tubulin, actin, the brain form of creatine kinase, neuron-specific enolase and protein 14-3-3 and calmodulin, all of which were identified on the basis of two-dimensional gel and peptide analyses. The 35 kDa protein product of membrane-specific mRNA was co-translationally processed in vitro by microsomal membranes, resulting in its cleavage to 33 kDa (and partial glycosylation). The 33 kDa processed protein (but not the 35 kDa precursor) was integrated into both dog pancreas and rat brain microsomal membranes. The occurrence of the enzymes and calmodulin as products of membrane-bound polyribosomal mRNA is discussed in the light of their presence on rat brain synaptic plasma membranes [Lim, Hall, Leung, Mahadevan & Whatley (1983) J. Neurochem. 41, 1177-1182] and their existence in a specific component of axonal flow. It is suggested that some of these translation products of the rough endoplasmic reticulum may represent proteins destined for the plasma membrane. However, the identity and location of the 35 kDa membrane-specific product (or its processed form) still remain unestablished.

scholarly journals Effects of Synaptic Plasma Membranes on Release of Acetylcholine from Synaptic Vesicles

1978 ◽  
Vol 28 (3) ◽  
pp. 339-343 ◽  
Author(s):  
Che-Hui Kuo ◽  
Seiji Ichida ◽  
Fumiaki Hata ◽  
Hiroshi Yoshida
Keyword(s):  
Synaptic Vesicles ◽  
Plasma Membranes ◽  
Synaptic Plasma Membranes ◽  
Release Of Acetylcholine
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