Partial purification and properties of long-chain acyl-CoA hydrolase from rat brain cytosol

1984 ◽  
Vol 9 (11) ◽  
pp. 1571-1591 ◽  
Author(s):  
Augustine Y. Lin ◽  
Grace Y. Sun ◽  
Ron macQuarrie
Keyword(s):  
Rat Brain ◽  
Partial Purification ◽  
Purification And Properties ◽  
Chain Acyl ◽  
Coa Hydrolase ◽  
Brain Cytosol ◽  
Long Chain

Long-Chain Acyl-CoA Hydrolase from Rat Brain Cytosol: Purification, Characterization, and Immunohistochemical Localization

1996 ◽  
Vol 326 (1) ◽  
pp. 106-114 ◽  
Author(s):  
Junji Yamada ◽  
Takao Furihata ◽  
Hiroshi Tamura ◽  
Takafumi Watanabe ◽  
Tetsuya Suga
Keyword(s):  
Rat Brain ◽  
Immunohistochemical Localization ◽  
Chain Acyl ◽  
Coa Hydrolase ◽  
Brain Cytosol ◽  
Long Chain

Neuron-specific distribution of long-chain acyl-CoA hydrolase in rat brain

1998 ◽  
Vol 31 ◽  
pp. S135
Author(s):  
Junji Yamada ◽  
Yu Kuramochi ◽  
Michiko Hirata ◽  
Takafumi Watanabe ◽  
Tetsuya Suga
Keyword(s):  
Rat Brain ◽  
Specific Distribution ◽  
Chain Acyl ◽  
Coa Hydrolase ◽  
Long Chain

Long-chain acyl CoA's and free fatty acids in rat brain after head injury

1997 ◽  
Vol 237 ◽  
pp. S25
Author(s):  
H. Heller ◽  
J. Deutsch ◽  
A.D. Purdon ◽  
S.I. Rapoport ◽  
M. Horowitz ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Head Injury ◽  
Free Fatty Acids ◽  
Rat Brain ◽  
Chain Acyl ◽  
Long Chain

scholarly journals Purification and Properties of Long-Chain Acyl-Coenzyme-A Synthetase from Rat Liver

1979 ◽  
Vol 98 (1) ◽  
pp. 165-172 ◽  
Author(s):  
Takao TANAKA ◽  
Kohei HOSAKA ◽  
Minoru HOSHIMARU ◽  
Shosaku NUMA
Keyword(s):  
Rat Liver ◽  
Coenzyme A ◽  
Purification And Properties ◽  
Chain Acyl ◽  
Acyl Coenzyme A ◽  
Long Chain

Molecular Cloning and Expression of cDNAs Encoding Rat Brain and Liver Cytosolic Long-Chain Acyl-CoA Hydrolases

1997 ◽  
Vol 232 (1) ◽  
pp. 198-203 ◽  
Author(s):  
Junji Yamada ◽  
Takao Furihata ◽  
Noriko Iida ◽  
Takafumi Watanabe ◽  
Masakiyo Hosokawa ◽  
...  
Keyword(s):  
Rat Brain ◽  
Molecular Cloning ◽  
Cloning And Expression ◽  
Rat Brain And Liver ◽  
Chain Acyl ◽  
Long Chain

Carnitine palmitoyltransferases in pea leaf chloroplasts: partial purification, location, and properties

2000 ◽  
Vol 78 (3) ◽  
pp. 328-335 ◽  
Author(s):  
Christine Masterson ◽  
Clifford Wood
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Carnitine Palmitoyltransferase ◽  
Chloroplast Envelope ◽  
Partial Purification ◽  
Western Blots ◽  
Chain Acyl ◽  
Long Chain ◽  
Inner Envelope

Carnitine palmitoyltransferase (EC 2.3.1.21), an enzyme that catalyses the reversible transfer of activated long-chain acyl groups between CoASH and L-carnitine, has been confirmed in pea leaf chloroplasts. This enzyme is bound to the chloroplast inner envelope membrane and has two isoforms, one bound to the outside (cytosol side) of the inner envelope and one bound to the inside (stromal side) of the inner envelope. Malonyl CoA inhibited the activity of the outer carnitine palmitoyltransferase, while stimulating the activity of the inner isoform and may be a regulator of these enzymes in vivo. Carnitine palmitoyltransferase was solubilized from the chloroplast envelope by detergent treatment and the two isoforms separated by Q-Sepharose anion exchange chromatography. Both proteins were immunochemically observed by probing Western blots of sodium dodecyl sulfate - polyacrylamide gel electrophoresis gels using an anti-beef heart mitochondrial carnitine palmitoyltransferase polyclonal antibody. The monomeric molecular mass of the protein recognized by this antibody was approximately 20 kDa. This 20-kDa protein also bound3H-carnitine. Both isoforms had broad acyl CoA substrate specificities, but showed increased activity with desaturated long-chain acyl CoAs, exhibiting a preference for linolenoyl CoA. A role for carnitine palmitoyltransferase in the shuttling of fatty acids across the chloroplast envelope is suggested.Key words: Pisum sativum, chloroplasts, carnitine palmitoyltransferase, fatty acid metabolism, eukaryotic pathway, membrane transport.

Purification and Properties of Calmodulin-Lysine N-Methyltransferase from Rat Brain Cytosol

1987 ◽  
Vol 48 (4) ◽  
pp. 1201-1208 ◽  
Author(s):  
Hideo Morino ◽  
Tatsuo Kawamoto ◽  
Masaharu Miyake ◽  
Yasuo Kakimoto
Keyword(s):  
Rat Brain ◽  
Purification And Properties ◽  
Brain Cytosol
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