Differential regional distribution of mucopolysaccharides in the human epiphyseal cartilage matrix in normal and pathologic conditions

1967 ◽  
Vol 342 (3) ◽  
pp. 274-281 ◽  
Author(s):  
C. Bona ◽  
V. Stanescu ◽  
D. Streja
Keyword(s):  
Regional Distribution ◽  
Cartilage Matrix ◽  
Epiphyseal Cartilage

Effects of EDTA, Hyaluronidase and Collagenase on Epiphyseal Cartilage Matrix

1968 ◽  
Vol 26 ◽  
pp. 56-57
Author(s):  
H. Clarke Anderson ◽  
Priscilla R. Coulter
Keyword(s):  
Light And Electron Microscopy ◽  
Matrix Vesicles ◽  
Cartilage Matrix ◽  
Collagen Fibrils ◽  
Epiphyseal Cartilage ◽  
Dense Matrix ◽  
Type Iv ◽  
Bovine Testicular Hyaluronidase ◽  
The Matrix ◽  
Testicular Hyaluronidase

Epiphyseal cartilage matrix contains fibrils and particles of at least 5 different types: 1. Banded collagen fibrils, present throughout the matrix, but not seen in the lacunae. 2. Non-periodic fine fibrils <100Å in diameter (Fig. 1), which are most notable in the lacunae, and may represent immature collagen. 3. Electron dense matrix granules (Fig. 1) which are often attached to fine fibrils and collagen fibrils, and probably contain protein-polysaccharide although the possibility of a mineral content has not been excluded. 4. Matrix vesicles (Fig. 2) which show a selective distribution throughout the epiphysis, and may play a role in calcification. 5. Needle-like apatite crystals (Fig. 2).Blocks of formalin-fixed epiphysis from weanling mice were digested with the following agents in 0.1M phosphate buffer: a) 5% ethylenediaminetetraacetate (EDTA) at pH 8.3, b) 0.015% bovine testicular hyaluronidase (Sigma, type IV, 750 units/mg) at pH 5.5, and c) 0.1% collagenase (Worthington, chromatograhically pure, 200 units/mg) at pH 7.4. All digestions were carried out at 37°C overnight. Following digestion tissues were examined by light and electron microscopy to determine changes in the various fibrils and particles of the matrix.

RADIOAUTOGRAPHIC STUDY OF S35O4 UPTAKE BY EPIPHYSEAL CARTILAGE IN EXPERIMENTAL LATHYRISM

1962 ◽  
Vol 40 (1) ◽  
pp. 565-570 ◽  
Author(s):  
Yurika K. Shintani ◽  
H. E. Taylor
Keyword(s):  
Normal Diet ◽  
Cartilage Matrix ◽  
Epiphyseal Cartilage ◽  
Acid Mucopolysaccharides ◽  
The Matrix ◽  
Endochondral Growth ◽  
Experimental Lathyrism

Disturbances in the uptake of radiosulphate were observed in radioautographs of the epiphysis of rats made lathyritic by giving either beta-aminopropionitrile or semicarbazide. There was a decreased uptake of sulphate which became more marked as the lesions advanced and an increased uptake was noted as the lesions regressed when the rats were returned to a normal diet. In normal rats, the radio-sulphate shifted to the zone of calcifying cartilage by the 4th day postinjection and, by the 7th day, it was concentrated over the ossifying trabeculae. In lathyrism this shift was delayed and the radiosulphate image was still concentrated in the cartilage matrix at days 4 and 7 postinjection. It is believed these findings reflect a disturbance in the matrix acid mucopolysaccharides and an interference with endochondral growth in lathyrism.

Femoral epiphyseal cartilage matrix changes at predilection sites of equine osteochondrosis: Quantitative MRI, second-harmonic microscopy, and histological findings

2016 ◽  
Vol 34 (10) ◽  
pp. 1743-1752 ◽  
Author(s):  
Gabrielle Martel ◽  
Charles-André Couture ◽  
Guillaume Gilbert ◽  
Stéphane Bancelin ◽  
Hélène Richard ◽  
...  
Keyword(s):  
Second Harmonic ◽  
Cartilage Matrix ◽  
Quantitative Mri ◽  
Epiphyseal Cartilage ◽  
Histological Findings ◽  
Equine Osteochondrosis

scholarly journals STUDIES ON ULTRASTRUCTURAL IDENTIFICATION AND DISTRIBUTION OF PROTEIN-POLYSACCHARIDE IN CARTILAGE MATRIX

1967 ◽  
Vol 32 (2) ◽  
pp. 365-377 ◽  
Author(s):  
Victor J. Matukas ◽  
Bernard J. Panner ◽  
J. Lowell Orbison
Keyword(s):  
Articular Cartilage ◽  
Cartilage Matrix ◽  
Epiphyseal Cartilage ◽  
Ground Substance ◽  
Dense Matrix ◽  
Chick Embryos ◽  
Chemical Analyses ◽  
Electron Microscopic ◽  
Colloidal Iron ◽  
The Matrix

Previous reports on the ultrastructure of cartilage matrix have described fibers, amorphous ground substance and, in some instances, dense matrix granules. The fibers are presumably collagen, but the nature of the granules is unknown. The primary purpose of this study has been to investigate the ultrastructure of cartilage matrix ih chick embryos with particular emphasis on the distribution and composition of these granules. In matrix of the zone of articular cartilage, mature collagen fibers can be seen but granules are not present. In matrix of all other zones of cartilage, fibers are smaller and granules are present. When the matrix of epiphyseal cartilage is compared to that of the zone of hypertrophic cells, fibers are similar but the granules in the latter zone are larger and more numerous. The granules in both zones were digested by hyaluronidase and positive to colloidal iron staining. Chemical analyses of cartilage from these zones indicate the hexosamine and radiosulfate content of the zone of hypertrophic cells to be higher than that of the zone of epiphyseal cartilage. The increased hexosamine was shown by column chromatography to be principally sulfated mucopolysaccharide, thereby indicating a direct correlation between size and number of granules and sulfated mucopolysaccharide content in the two zones. These data and the results of the electron microscopic histochemical studies are consistent with the concept that the granules in cartilage matrix contain acidic mucopolysaccharide.

RADIOAUTOGRAPHIC STUDY OF S35O4 UPTAKE BY EPIPHYSEAL CARTILAGE IN EXPERIMENTAL LATHYRISM

1962 ◽  
Vol 40 (5) ◽  
pp. 565-570 ◽  
Author(s):  
Yurika K. Shintani ◽  
H. E. Taylor
Keyword(s):  
Normal Diet ◽  
Cartilage Matrix ◽  
Epiphyseal Cartilage ◽  
Acid Mucopolysaccharides ◽  
The Matrix ◽  
Endochondral Growth ◽  
Experimental Lathyrism

Disturbances in the uptake of radiosulphate were observed in radioautographs of the epiphysis of rats made lathyritic by giving either beta-aminopropionitrile or semicarbazide. There was a decreased uptake of sulphate which became more marked as the lesions advanced and an increased uptake was noted as the lesions regressed when the rats were returned to a normal diet. In normal rats, the radio-sulphate shifted to the zone of calcifying cartilage by the 4th day postinjection and, by the 7th day, it was concentrated over the ossifying trabeculae. In lathyrism this shift was delayed and the radiosulphate image was still concentrated in the cartilage matrix at days 4 and 7 postinjection. It is believed these findings reflect a disturbance in the matrix acid mucopolysaccharides and an interference with endochondral growth in lathyrism.

scholarly journals COMPARISON OF THE EFFECTS OF PAPAIN AND VITAMIN A ON CARTILAGE

1960 ◽  
Vol 111 (5) ◽  
pp. 705-718 ◽  
Author(s):  
Lewis Thomas ◽  
Robert T. McCluskey ◽  
Jacobus L. Potter ◽  
Gerald Weissmann
Keyword(s):  
Vitamin A ◽  
Chondroitin Sulfate ◽  
Cartilage Matrix ◽  
Epiphyseal Cartilage ◽  
Blue Staining ◽  
Daily Injection ◽  
Alcian Blue Staining ◽  
The Matrix ◽  
Cartilage Cells ◽  
Ear Cartilage

The administration of large amounts of vitamin A to rabbits has been shown to result in depletion of cartilage matrix. The normal basophilic, metachromatic, and Alcian blue staining properties of the matrix are lost, especially in articular and epiphyseal cartilage. The cartilage cells remain intact, but are reduced in size. These changes sometimes appeared as early as 48 hours after the initiation of daily injection of 1 million units of vitamin A, and were usually well established by 5 days. Some rabbits failed to show changes in cartilage, even after 5 daily injections. Increased amounts of material presumed to be chondroitin sulfate were present in the sera of vitamin A-treated rabbits, usually by 72 hours after the first injection. This was demonstrated by a turbidimetric procedure using hexamminecobaltic chloride. In rabbits given sulfur-35 (Na2S35O4) 5 days before the initiation of vitamin A treatment, it was shown that sulfur-35 was lost from articular and epiphyseal cartilage. This was associated with an increase in the non-dialyzable sulfur-35 in both serum and in the cobalt-precipitable material. These rabbits also excreted more sulfur-35 than rabbits not given vitamin A. There was a reduction in sulfur-35 activity in chondromucoprotein extracted from the ear cartilage of vitamin A-treated rabbits. The changes are interpreted as indicating that the administration of large amounts of vitamin A to rabbits results in removal of chondroitin sulfate from cartilage matrix. The administration of small amounts of crude papain causes histologic changes in cartilage that are remarkably similar to those seen in vitamin A-treated rabbits. The possibility is suggested that the changes in cartilage produced by administration of vitamin A to rabbits may be the result of activation of a proteolytic enzyme or enzymes, with properties similar to those of papain.

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