Elements of theS-gene complex VI. Mutations of the self-incompatibility gene, pseudo-compatibility and origin of new self-incompatibility alleles

Genetica ◽  
1970 ◽  
Vol 41 (1) ◽  
pp. 477-516 ◽  
Author(s):  
Kamla Kant Pandey
Keyword(s):  
The Self ◽  
Gene Complex ◽  
Self Incompatibility ◽  
Incompatibility Alleles ◽  
Thes Gene

scholarly journals Incompatibility Alleles in Portuguese Hazelnut Landraces

ISRN Agronomy ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
Sandra Martins ◽  
Mercè Rovira ◽  
Ana Paula Silva ◽  
Valdemar Carnide
Keyword(s):  
Higher Plants ◽  
Pollen Tubes ◽  
The Self ◽  
Single Locus ◽  
Self Incompatibility ◽  
Multiple Alleles ◽  
Breeding Programmes ◽  
Incompatibility Alleles ◽  
Related Individuals ◽  
New Cultivars

In many higher plants, selffertilization and genetically related individuals are prevented by pollen-stigma incompatibility. In the genus Corylus, incompatibility is of the sporophytic type and controlled by a single locus with multiple alleles. The objective of this study is to identify the S-alleles present in a collection of Portuguese landraces in order to select the most appropriate landraces for establishment of future orchards and for breeding programmes. Ten major Portuguese hazelnut landraces were submitted to controlled pollinations in the field, with 18 genotypes whose S-alleles are known. The pollen tubes were observed at 100X under a florescence microscope to evaluate their development. Three landraces were revealed to have S2 allele, two have S5, and four have one of the S3, S5, S10, and S18 alleles. One landrace was compatible with the 18 S-alleles tested and for two landraces, it was possible to identify both alleles. The information of the self-incompatibility relationship between these old cultivars is obviously useful for selecting the most suitable pollinators for planning new orchards and for new cultivars development.

scholarly journals Detecting and determining self-incompatibility alleles in almond genotypes using molecular method.

2019 ◽  
Author(s):  
Sorush Niknamian
Keyword(s):  
The Self ◽  
Self Incompatibility ◽  
Base Pairs ◽  
Inhibitory System ◽  
S Alleles ◽  
Study Population ◽  
Incompatibility Alleles ◽  
Pcr Method ◽  
Polymerase Chain ◽  
New Alleles

Abstract One of the problems in almond production is self-incompatibility in this plant, which is considered as an important improvement point for this tree. Self-incompatibility causes non-uniformity and garden management problems. Most cultivars of almonds have gametophytic self-incompatibility that is controlled by a multi-allelic gene site. The inoculation inhibitor factor in this inhibitory system is the stop of pollen tube growth in the style. This study aims to detect and determine the self-compatible genotype from among the studied samples and determine the self-incompatibility alleles in the studied masses. For the experiment, the leaf samples were collected from 100 almond genotypes that had good products in recent years. The DNA of young leaf samples in these genotypes was extracted using Gept and Celeg (1989) method with a few changes. Today, various methods have been invented for detecting the genotypes and self-compatible cultivars from selfincompatible cultivars as well as S alleles in almonds, including the PCR method. Therefore, in order to detect S alleles in different almond and some hybrid genotypes, the exclusive primer pairs, including AS1II-AmyC5R, ConF-ConR and Cebador2-Cebador8, were used in the polymerase chain reaction. All of the primers have been used by other researchers to detect almond alleles and the effectiveness of these pairs of primers was confirmed in this experiment. Using the AS1IIAmyC5R and Cebador2-Cebador8 primers, the Sf allele with the size of 1200 base pairs was detected. Using the ConF-ConR pair of primer, the S1, S2, S3, S10, S11, S23, and S31 alleles were detected in the self-incompatible samples. Using AS1II-AmyC5R pair of primer, the known alleles of S3, Sf, S2, S1, S5, S10, S11, S23, and S13 were detected. The other bands obtained from the PCR were related to the known self-incompatibility alleles that might be considered as new alleles. In the study population in this research, S1, S2, S3, and S11 alleles had higher frequency.

Assessment of Mating System in Helianthus annuus and H. petiolaris (Asteraceae) Populations

Helia ◽  
2020 ◽  
Vol 43 (72) ◽  
pp. 15-32
Author(s):  
Agustina Gutierrez ◽  
Daiana Scaccia Baffigi ◽  
Monica Poverene
Keyword(s):  
Gene Flow ◽  
Helianthus Annuus ◽  
Genetic System ◽  
The Self ◽  
Self Incompatibility ◽  
S Alleles ◽  
Self Fertilization ◽  
Self Compatibility ◽  
Incompatibility Alleles ◽  
Crop Area

AbstractHelianthus annuus subsp. annuus and H. petiolaris are wild North American species that have been naturalized in central Argentina. They have a sporophytic self-incompatibility genetic system that prevent self-fertilization but the occurrence of self-compatible plants in Argentina was observed in both species and could in part explain their highly invasive ability. Their geographical distribution coincides with the major crop area. The domestic sunflower is self-compatible, can hybridize with both species and presents a considerable amount of gene flow. The aim of this study is to understand the self-incompatibility mechanism in both wild Helianthus species. Reciprocal crossing and seed production were used to identify self-compatible genotypes, the number and distribution of self-incompatibility alleles within populations and the type and extent of allelic interactions in the pollen and pistil. The behaviour of S alleles within each population was explained by five functional S alleles and one non-functional allele in each species, differing in their presence and frequency within accessions. In both species, the allelic interactions were of dominance/recessiveness and codominance in pollen, whereas it was only codominance in the pistil. Inbreeding effects in wild materials appeared in the third generation of self-pollination, with lethal effects in most plants. The number of S alleles is low and they behave in a similar way of other Asteraceae species. The self-compatibility was addressed to non-functional S alleles introgressed in wild Helianthus plants through gene flow from self-compatible sunflower.

scholarly journals Molecular characteristics of S-RNase alleles as the determinant of self-incompatibility in the style of Fragaria viridis

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jianke Du ◽  
Chunfeng Ge ◽  
Tingting Li ◽  
Sanhong Wang ◽  
Zhihong Gao ◽  
...  
Keyword(s):  
Diploid Species ◽  
The Self ◽  
Molecular Characteristics ◽  
Self Incompatibility ◽  
Intraspecific Hybridization ◽  
Noncoding Sequences ◽  
Family Analysis ◽  
The Family ◽  
Genotype Identification ◽  
Rnase T2

AbstractStrawberry (Fragaria spp.) is a member of the Rosoideae subfamily in the family Rosaceae. The self-incompatibility (SI) of some diploid species is a key agronomic trait that acts as a basic pollination barrier; however, the genetic mechanism underlying SI control in strawberry remains unclear. Two candidate S-RNases (Sa- and Sb-RNase) identified in the transcriptome of the styles of the self-incompatible Fragaria viridis 42 were confirmed to be SI determinants at the S locus following genotype identification and intraspecific hybridization using selfing progenies. Whole-genome collinearity and RNase T2 family analysis revealed that only an S locus exists in Fragaria; however, none of the compatible species contained S-RNase. Although the results of interspecific hybridization experiments showed that F. viridis (SI) styles could accept pollen from F. mandshurica (self-compatible), the reciprocal cross was incompatible. Sa and Sb-RNase contain large introns, and their noncoding sequences (promotors and introns) can be transcribed into long noncoding RNAs (lncRNAs). Overall, the genus Fragaria exhibits S-RNase-based gametophytic SI, and S-RNase loss occurs at the S locus of compatible germplasms. In addition, a type of SI-independent unilateral incompatibility exists between compatible and incompatible Fragaria species. Furthermore, the large introns and neighboring lncRNAs in S-RNase in Fragaria could offer clues about S-RNase expression strategies.

scholarly journals Cytological Observation and Transcriptome Comparative Analysis of Self-Pollination and Cross-Pollination in Dendrobium Officinale

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 432
Author(s):  
Yaling Chen ◽  
Benchang Hu ◽  
Fantao Zhang ◽  
Xiangdong Luo ◽  
Jiankun Xie
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Dendrobium Officinale ◽  
Self Incompatibility ◽  
Related Gene ◽  
Cytological Observation ◽  
Lectin Receptor ◽  
Cross Pollination ◽  
Self Pollination ◽  
Medicinal Value

Dendrobium officinale is a rare and traditional medicinal plant with high pharmacological and nutritional value. The self-incompatibility mechanism of D. officinale reproductive isolation was formed in the long-term evolution process, but intraspecific hybridization of different germplasm resources leads to a large gap in the yield, quality, and medicinal value of D. officinale. To investigate the biological mechanism of self-incompatibility in D. officinale, cytological observation and the transcriptome analysis was carried out on the samples of self-pollination and cross-pollination in D. officinale. Results for self-pollination showed that the pollen tubes could grow in the style at 2 h, but most of pollen tubes stopped growing at 4 h, while a large number of cross-pollinated pollen tubes grew along the placental space to the base of ovary, indicating that the self-incompatibility of D. officinale may be gametophyte self-incompatibility. A total of 63.41 G basesum of D. officinale style samples from non-pollinated, self-pollination, and cross-pollination by RNA-seq were obtained, and a total of 1944, 1758, and 475 differentially expressed genes (DEGs) in the comparison of CK (non-pollinated) vs. HF (cross-pollination sample), CK vs. SF (self-pollination sample) and SF vs. HF were identified, respectively. Forty-one candidate genes related to self-incompatibility were found by function annotation of DEGs, including 6 Ca2+ signal genes, 4 armed repeat containing (ARC) related genes, 11 S-locus receptor kinase (SRK) related genes, 2 Exo70 family genes, 9 ubiquitin related genes, 1 fatty acid related gene, 6 amino acid-related genes, 1 pollen-specific leucine-rich repeat extensin-like protein (LRX) related gene and 1 lectin receptor-like kinases (RLKs) related gene, showed that self-incompatibility mechanism of D. officinale involves the interaction of multiple genes and pathways. The results can provide a basis for the study of the self-incompatibility mechanism of D. officinale, and provide ideas for the preservation and utilization of high-quality resources of D. officinale.

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