Visceral larva migrans: Examinations by means of enzyme-linked immunosorbent assay of human sera for antibodies to excretory-secretory antigens of the second-stage larvae ofToxocara canis

1982 ◽  
Vol 69 (1) ◽  
pp. 113-118 ◽  
Author(s):  
F. Knapen ◽  
J. Leusden ◽  
A. M. Polderman ◽  
J. H. Franchimont
Keyword(s):  
Immunosorbent Assay ◽  
Larva Migrans ◽  
Enzyme Linked Immunosorbent Assay ◽  
Visceral Larva Migrans ◽  
Second Stage ◽  
Human Sera

scholarly journals Enzyme-Linked Immunosorbent Assay To Differentiate the Antibody Responses of Animals Infected with Brucella Species from Those of Animals Infected with Yersinia enterocolitica O9

2003 ◽  
Vol 10 (4) ◽  
pp. 710-714 ◽  
Author(s):  
Janchivdorj Erdenebaatar ◽  
Balgan Bayarsaikhan ◽  
Masahisa Watarai ◽  
Sou-ichi Makino ◽  
Toshikazu Shirahata
Keyword(s):  
Yersinia Enterocolitica ◽  
Immunosorbent Assay ◽  
Brucella Abortus ◽  
Field Trial ◽  
Antibody Responses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Brucella Species ◽  
Serological Tests ◽  
Human Sera ◽  
Immunosorbent Assays

ABSTRACT Enzyme-linked immunosorbent assays using antigens extracted from Brucella abortus with n-lauroylsarcosine differentiated natural Brucella-infected animals from Brucella-vaccinated or Yersinia enterocolitica O9-infected animals. A field trial in Mongolia showed cattle, sheep, goat, reindeer, camel, and human sera without infection could be distinguished from Brucella-infected animals by conventional serological tests.

scholarly journals Sensitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies Against Typhus Rickettsiae, Rickettsia prowazekii and Rickettsia typhi

1977 ◽  
Vol 6 (2) ◽  
pp. 101-110
Author(s):  
Sidney Halle ◽  
Gregory A. Dasch ◽  
Emilio Weiss
Keyword(s):  
Immunosorbent Assay ◽  
Complement Fixation ◽  
Differential Susceptibility ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rickettsia Prowazekii ◽  
Rickettsia Typhi ◽  
Titration Curves ◽  
Ether Extraction ◽  
Human Sera

An enzyme-linked immunosorbent assay (ELISA) has been developed for the titration of rickettsial antibodies in human and animal sera. Two preparations of soluble typhus-group antigens were obtained from Rickettsia typhi and Rickettsia prowazekii by ether extraction: a standard antigen from infected yolk sacs (YS antigen) and one free of yolk sac contaminants from Renografin-purified rickettsiae (PR antigen). Rabbit, mouse, and guinea pig sera were obtained by immunization with viable purified R. typhi or R. prowazekii . Human sera were obtained from individuals who had recovered from laboratory infections with either typhus rickettsia months or years previously. Goat-derived anti-immunoglobulins were conjugated to alkaline phosphatase with glutaraldehyde. Although the PR and YS antigens gave equivalent antibody titers in the complement fixation test, the PR antigen was clearly superior in the ELISA. With this antigen, the titration curves of all antisera were linear over a wider range of serum concentrations and the titers were higher than with the YS antigen. With YS and PR antigens, ELISA titers were higher than those obtained by complement fixation by one and two orders of magnitude, respectively. In human sera, immunoglobulin G and immunoglobulin M antibodies were demonstrated by their respective anti-immunoglobulins and by differential susceptibility to ethanethiol. ELISA titers showed some type specificity, whereas none was observed in complement fixation tests. The ELISA is highly sensitive, reproducible, and easily adaptable to the various requirements of clinical and research laboratories.

Detection of Toscana Virus-Specific Immunoglobulins G and M by an Enzyme-Linked Immunosorbent Assay Based on Recombinant Viral Nucleoprotein

1999 ◽  
Vol 37 (6) ◽  
pp. 2010-2012 ◽  
Author(s):  
Maria Grazia Ciufolini ◽  
Cristiano Fiorentini ◽  
Paola di Bonito ◽  
Stefania Mochi ◽  
Colomba Giorgi
Keyword(s):  
Infected Mouse ◽  
Sensitivity And Specificity ◽  
Mouse Brain ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Toscana Virus ◽  
Human Sera

An enzyme-linked immunosorbent assay (ELISA) based on the recombinant Toscana virus nucleoprotein (rN) has been developed. Its sensitivity and specificity for the detection of virus-specific immunoglobulins G and M in human sera were similar to those of the ELISA that is based on an antigen extracted from infected mouse brain and that is routinely used for serodiagnosis.

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