Trypanosoma cruzi: Inhibition of host cell uptake of infective bloodstream forms by alpha-2-macroglobulin

1986 ◽  
Vol 72 (3) ◽  
pp. 323-329 ◽  
Author(s):  
Tania C. Araujo-Jorge ◽  
Elizabeth P. Sampaio ◽  
Wanderley Souza
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cell Uptake

scholarly journals Mammalian cell invasion and intracellular trafficking by Trypanosoma cruzi infective forms

2005 ◽  
Vol 77 (1) ◽  
pp. 77-94 ◽  
Author(s):  
Renato A. Mortara ◽  
Walter K. Andreoli ◽  
Noemi N. Taniwaki ◽  
Adriana B. Fernandes ◽  
Claudio V. da Silva ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Mammalian Cells ◽  
Parasitophorous Vacuole ◽  
Host Cells ◽  
Mammalian Host ◽  
Target Cells ◽  
Sylvatic Cycle ◽  
Final Destination ◽  
Different Strains

Trypanosoma cruzi, the etiological agent of Chagas’ disease, occurs as different strains or isolates that may be grouped in two major phylogenetic lineages: T. cruzi I, associated with the sylvatic cycle and T. cruzi II, linked to the human disease. In the mammalian host the parasite has to invade cells and many studies implicated the flagellated trypomastigotes in this process. Several parasite surface components and some of host cell receptors with which they interact have been identified. Our work focused on how amastigotes, usually found growing in the cytoplasm, can invade mammalian cells with infectivities comparable to that of trypomastigotes. We found differences in cellular responses induced by amastigotes and trypomastigotes regarding cytoskeletal components and actin-rich projections. Extracellularly generated amastigotes of T. cruzi I strains may display greater infectivity than metacyclic trypomastigotes towards cultured cell lines as well as target cells that have modified expression of different classes of cellular components. Cultured host cells harboring the bacterium Coxiella burnetii allowed us to gain new insights into the trafficking properties of the different infective forms of T. cruzi, disclosing unexpected requirements for the parasite to transit between the parasitophorous vacuole to its final destination in the host cell cytoplasm.

scholarly journals Ursolic acid promotes clearance of <em>Trypanosoma cruzi amastigotes </em>in the host cell

2020 ◽  
Author(s):  
Patricia Romano ◽  
María Vanrell ◽  
Santiago Martinez ◽  
Lucila Muñoz ◽  
Betiana Salassa
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Ursolic Acid

Possible integration of Trypanosoma cruzi kDNA minicircles into the host cell genome by infection

1994 ◽  
Vol 305 (2) ◽  
pp. 197-209 ◽  
Author(s):  
Antonio R.L. Teixeira ◽  
Enrique R. Argañaraz ◽  
Lucio H. Freitas ◽  
Zulmira G.M. Lacava ◽  
Jaime M. Santana ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cell Genome

scholarly journals Host Cell Lipid Bodies Triggered by Trypanosoma cruzi Infection and Enhanced by the Uptake of Apoptotic Cells Are Associated With Prostaglandin E2 Generation and Increased Parasite Growth

2011 ◽  
Vol 204 (6) ◽  
pp. 951-961 ◽  
Author(s):  
Heloisa D’Avila ◽  
Célio G. Freire-de-Lima ◽  
Natalia R. Roque ◽  
Livia Teixeira ◽  
Christina Barja-Fidalgo ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Prostaglandin E2 ◽  
Host Cell ◽  
Parasite Growth ◽  
Apoptotic Cells ◽  
Lipid Bodies ◽  
Cell Lipid ◽  
Cruzi Infection

scholarly journals Reprogramming of Trypanosoma cruzi metabolism triggered by parasite interaction with the host cell extracellular matrix

2019 ◽  
Vol 13 (2) ◽  
pp. e0007103 ◽  
Author(s):  
Eliciane C. Mattos ◽  
Gisele Canuto ◽  
Nubia C. Manchola ◽  
Rubens D. M. Magalhães ◽  
Thomas W. M. Crozier ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Trypanosoma Cruzi ◽  
Host Cell

scholarly journals Inhibition of Trypanosoma cruzi growth in mammalian cells by purine and pyrimidine analogs.

1996 ◽  
Vol 40 (11) ◽  
pp. 2455-2458 ◽  
Author(s):  
J Nakajima-Shimada ◽  
Y Hirota ◽  
T Aoki
Keyword(s):  
Trypanosoma Cruzi ◽  
Growth Inhibition ◽  
Host Cell ◽  
Culture System ◽  
Mammalian Cells ◽  
Screening Method ◽  
Host Cells ◽  
Mammalian Host ◽  
Dependent Manner ◽  
Pyrimidine Analogs

Trypanosoma cruzi, the causative agent of Chagas' disease, exhibits two different developmental stages in mammals, the amastigote, an intracellular form that proliferates in the cytoplasm of host cells, and the trypomastigote, an extracellular form that circulates in the bloodstream. We have already established an in vitro culture system using mammalian host cells (HeLa) infected with T. cruzi in which the time course of parasite growth is determined quantitatively. We adopted this system for the screening of anti-T. cruzi agents that would ideally prove to be effective against trypanosomes with no toxicity to the host cell. Of the purine analogs tested, allopurinol markedly inhibited the growth of amastigotes in a dose-dependent manner, with no lethal effect on trypomastigotes. 3'-Deoxyinosine and 3'-deoxyadenosine also suppressed T. cruzi growth inside the host cell, with the concentrations causing 50% growth inhibition being 10 and 5 microM, respectively, in contrast to a concentration causing 50% growth inhibition of 3 microM for allopurinol. Among the pyrimidine analogs examined, 3'-azido-3'-deoxythymidine (zidovudine) significantly reduced the growth of the parasite at concentrations as low as 1 microM. The anti-human immunodeficiency virus agents 2',3'-dideoxyinosine and 2',3'-dideoxyadenosine caused a decrease in amastigote growth, while 2',3'-dideoxycytidine and 2',3'-dideoxyuridine had no inhibitory effect. When Swiss 3T3 fibroblasts were used as host cells, allopurinol, 3'-deoxyinosine, 3'-deoxyadenosine, and 3'-azid-3'-deoxythymidine also markedly inhibited T. cruzi proliferation. These results indicate that our culture system is useful as a primary screening method for candidate compounds against T. cruzi on the basis of two criteria, namely, intracellular replication by the parasite and host-cell infection rate.

Elucidating Host Cell Uptake by Malaria Parasites

2019 ◽  
Vol 35 (5) ◽  
pp. 333-335 ◽  
Author(s):  
Brendan Elsworth ◽  
Caroline D. Keroack ◽  
Manoj T. Duraisingh
Keyword(s):  
Host Cell ◽  
Cell Uptake ◽  
Malaria Parasites
Sign in / Sign up

Export Citation Format

Share Document