Plaque assay of alfalfa looper nuclear polyhedrosis virus on the TN-368 cell line

1979 ◽  
Vol 5 (1) ◽  
pp. 1033-1035 ◽  
Author(s):  
W. Fred Hink ◽  
Elaine M. Strauss
Keyword(s):  
Cell Line ◽  
Nuclear Polyhedrosis Virus ◽  
Plaque Assay ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Alfalfa Looper

A cloned cell line ofSpodopteta exiguahas a highly increased susceptibility to theSpodoptera exiguanuclear polyhedrosis virus

1995 ◽  
Vol 41 (12) ◽  
pp. 1111-1116 ◽  
Author(s):  
Koyu Hara ◽  
Masako Funakoshi ◽  
Takeshi Kawarabata
Keyword(s):  
Cell Line ◽  
Spodoptera Exigua ◽  
Nuclear Polyhedrosis Virus ◽  
Plaque Assay ◽  
Virus Production ◽  
Parental Cell ◽  
Parental Cell Line ◽  
Parent Cell ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

A cloned cell line designated Se301, derived from a continuous Se3FH cell line of the beet army worm Spodoptera exigua, showed 10 times greater sensitivity when tested with the plaque assay to a S. exigua nuclear polyhedrosis virus (SeNPV) as compared with the parent cell line. Nearly 100% of Se301 cells were infected with the plaque purified isolate SeNPV-I1 or the wild isolate SeNPV-IW. Plaques were first detected 3–4 days in the Se301 cell line, which was 1–2 days faster than the Se3FH cell line. The size of plaques formed in the Se301 cell line was markedly larger than that of the parental cell line. At 12 h post infection, the production of the extracellular viruses (ECVs) in the Se301 cell line infected with either SeNPV-I1 or SeNPV-IW was 20–40 times greater than that in the Se3FH cell line. In contrast, Se301 cells infected with SeNPV-I1 or SeNPV-IW produced polyhedral inclusion bodies (PIBs) at lower levels compared with Se3FH cells. The isolate SeNPV-I1 consistently produced more ECVs and PIBs in both the Se301 and Se3FH cell lines than the isolate SeNPV-IW.Key words: Spodoptera exigua, nuclear polyhedrosis virus, cloned cell line, increased virus sensitivity, virus production.

Infection of Spodoptera frugiperda and Choristoneura fumiferana cell lines with the baculovirus Choristoneura fumiferana nuclear polyhedrosis virus

1993 ◽  
Vol 39 (10) ◽  
pp. 932-940 ◽  
Author(s):  
J. Jian Liu ◽  
Eric B. Carstens
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Spodoptera Frugiperda ◽  
Nuclear Polyhedrosis Virus ◽  
Choristoneura Fumiferana ◽  
Spruce Budworm ◽  
Extracellular Virus ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Alfalfa Looper

The growth properties of cell lines derived from Spodoptera frugiperda (alfalfa looper) and Choristoneura fumiferana (spruce budworm) were investigated. The data demonstrated that the spruce bud worm cell line grew more slowly than the alfalfa looper cell line, and this reduced growth rate appeared to affect the rate of baculovirus replication in infected cells. Trypsinizing the spruce budworm cells or varying the multiplicity of infection did not greatly influence the rate of viral replication. Autographa californica nuclear polyhedrosis virus was able to replicate its DNA and synthesize late and very late proteins in both cell lines but did not produce infectious extracellular virus in the spruce budworm cell line. The replication cycle of C. fumiferana nuclear polyhedrosis virus did not produce late proteins or infectious extracellular virus in the alfalfa looper cells. The results indicate that S. frugiperda cells are nonpermissive for the C. fumiferana nuclear polyhedrosis virus but C. fumiferana cells are semipermissive for the A. californica nuclear polyhedrosis virus, resulting in an abortive infection.Key words: baculovirus, host specificity, AcMNPV, CfMNPV, spruce budworm.

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