Role of oxygen intermediates in cytotoxicity: Studies in chronic granulomatous disease

Inflammation ◽  
1993 ◽  
Vol 17 (1) ◽  
pp. 77-92 ◽  
Author(s):  
Robert L. Roberts ◽  
Bonnie J. Ank ◽  
Michael W. Fanger ◽  
Li Shen ◽  
E. Richard Stiehm
Keyword(s):  
Chronic Granulomatous Disease ◽  
Granulomatous Disease ◽  
Oxygen Intermediates ◽  
Cytotoxicity Studies

scholarly journals Estrogen binding by leukocytes during phagocytosis,.

1977 ◽  
Vol 145 (4) ◽  
pp. 983-998 ◽  
Author(s):  
S J Klebanoff
Keyword(s):  
Chronic Granulomatous Disease ◽  
Reaction Mechanisms ◽  
Dehydrogenase Deficiency ◽  
Cell Types ◽  
Neutrophil Function ◽  
Granulomatous Disease ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Estrogen Binding ◽  
Two Populations

Estradiol binds covalently to normal leukocytes during phagocytosis. The binding involves three cell types, neutrophils, eosinophils, and monocytes and at least two reaction mechanisms, one involving the peroxidase of neutrophils and monocytes (myeloperoxidase [MPO]) and possibly the eosinophil peroxidase, and the second involving catalase. Binding is markedly reduced when leukocytes from patients with chronic granulomatous disease (CGD), severe leukocytic glucose 6-phosphate dehydrogenase deficiency, and familial lipochrome histiocytosis are employed and two populations of neutrophils, one which binds estradiol and one which does not, can be demonstrated in the blood of a CGD carrier. Leukocytes from patients with hereditary MPO deficiency also bind estradiol poorly although the defect is not as severe as in CGD. These findings are discussed in relation to the inactivation of estrogens during infection and the possible role of estrogens in neutrophil function.

Further evaluation of luminol-enhanced luminescence in the diagnosis of disorders of leukocyte oxidative metabolism: role of myeloperoxidase.

1983 ◽  
Vol 29 (3) ◽  
pp. 513-515 ◽  
Author(s):  
M S Cohen ◽  
P S Shirley ◽  
L R DeChatelet
Keyword(s):  
Chronic Granulomatous Disease ◽  
Oxidative Metabolism ◽  
Clinical Consequence ◽  
Granulomatous Disease ◽  
Myeloperoxidase Deficiency ◽  
Luminescence Assay ◽  
Diagnostic Confusion ◽  
Enhanced Luminescence ◽  
Hereditary Nature

Abstract Chemiluminescence can be used to identify defects in the oxidative metabolism of granulocytes. This procedure has recently been adopted for use with microliter quantities of whole blood, appropriate for prenatal or neonatal study. Although the contribution of myeloperoxidase to the chemiluminescence assay has been noted, the possible diagnostic confusion between chronic granulomatous disease of childhood (which is rare and severe) and myeloperoxidase deficiency (which is common and of little clinical consequence) has not been stressed. We report a father and his infant daughter whose cells emitted no light in the luminol-enhanced luminescence assay; both patients are totally peroxidase deficient. These results emphasize the hereditary nature of myeloperoxidase deficiency, and the possibility for erroneous diagnosis of chronic granulomatous disease of childhood based on the luminol-enhanced luminescence test.

Liver involvement in chronic granulomatous disease: the role of ultrasound in diagnosis and treatment.

Radiology ◽  
1984 ◽  
Vol 153 (1) ◽  
pp. 117-121 ◽  
Author(s):  
L A Garel ◽  
D M Pariente ◽  
C Nezelof ◽  
V J Barral ◽  
C Aboulker ◽  
...  
Keyword(s):  
Chronic Granulomatous Disease ◽  
Diagnosis And Treatment ◽  
Liver Involvement ◽  
Granulomatous Disease

scholarly journals Hyperinflammation in chronic granulomatous disease and anti-inflammatory role of the phagocyte NADPH oxidase

2008 ◽  
Vol 30 (3) ◽  
pp. 255-271 ◽  
Author(s):  
Michela G. Schäppi ◽  
Vincent Jaquet ◽  
Dominique C. Belli ◽  
Karl-Heinz Krause
Keyword(s):  
Nadph Oxidase ◽  
Chronic Granulomatous Disease ◽  
Granulomatous Disease ◽  
Phagocyte Nadph Oxidase ◽  
Anti Inflammatory

Role of Flow Cytometry in the Diagnosis of Chronic Granulomatous Disease: the Egyptian Experience

2016 ◽  
Vol 36 (6) ◽  
pp. 610-618 ◽  
Author(s):  
Rabab El Hawary ◽  
Safa Meshaal ◽  
Caroline Deswarte ◽  
Nermeen Galal ◽  
Mahitab Abdelkawy ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Chronic Granulomatous Disease ◽  
Granulomatous Disease

scholarly journals Effect of phagocytosis on the reduced soluble sulfhydryl content of human granulocytes

Blood ◽  
1977 ◽  
Vol 50 (6) ◽  
pp. 1023-1030 ◽  
Author(s):  
DS Mendelson ◽  
EN Metz ◽  
AL Jr Sagone
Keyword(s):  
Chronic Granulomatous Disease ◽  
Reduced Glutathione ◽  
Disease Patient ◽  
Granulomatous Disease ◽  
Normal Cells ◽  
Nitroblue Tetrazolium Reduction ◽  
Human Granulocytes ◽  
Load Exposure ◽  
Phagocytic Response

Abstract The role of reduced glutathione in relation to hexose monophosphate shunt activity and peroxide detoxification has been well established in human erythrocytes. Less is known about the content of reduced glutathione in phagocytic leukocytes and the changes that occur during functional activity. We have measured the reduced sulfhydryl content of normal resting human granulocytes and of cells isolated from a patient with chronic granulomatous disease. Normal cells and those from the patient with chronic granulomatous disease contained similar concentrations of reduced sulfhydryls. Stimulation of a phagocytic response by incubation with opsonized zymosan particles resulted in prompt and nearly complete depletion of intracellular glutathione from normal granulocytes. This fall in reduced glutathione concentration was dependent on the phagocytic load. Exposure of chronic granulomatous disease granulocytes to a similar phagocytic load resulted in a slower and less complete fall in reduced glutathione. In normal cells, those from the chronic granulomatous disease patient, and those from an obligate carrier of the disease, the decrement in reduced glutathione during phagocytosis was correlated with oxidation of 14C-1-glucose and 14C-formate, nitroblue tetrazolium reduction, and the chemiluminescence phenomenon.

The role of oxidants and vitamin C on neutrophil apoptosis and clearance

2004 ◽  
Vol 32 (3) ◽  
pp. 499-501 ◽  
Author(s):  
M.C.M. Vissers ◽  
M.B. Hampton
Keyword(s):  
Vitamin C ◽  
Chronic Granulomatous Disease ◽  
Surface Expression ◽  
Neutrophil Apoptosis ◽  
Granulomatous Disease ◽  
Probable Outcome ◽  
Induced Apoptosis ◽  
Deficient Mice ◽  
Surface Changes

We have investigated the role of neutrophil oxidants in the surface changes that result in recognition and uptake of neutrophils by macrophages. We have shown that H2O2 produced by stimulated neutrophils is essential for the surface expression of phosphatidylserine. This does not occur in neutrophils from mice with chronic granulomatous disease and may explain the formation of granuloma in this condition. We have also investigated the role of intracellular vitamin C on neutrophil apoptosis. Cells from vitamin C-deficient mice were found to be less likely to undergo both spontaneous and oxidant-induced apoptosis, with eventual necrosis being the most probable outcome.

scholarly journals The Role of Surgery in the Management of Patients With Refractory Chronic Granulomatous Disease Colitis

2013 ◽  
Vol 56 (5) ◽  
pp. 609-614 ◽  
Author(s):  
Melissa M. Alvarez-Downing ◽  
Natasha Kamal ◽  
Suzanne M. Inchauste ◽  
Sajneet K. Khangura ◽  
Harry L. Malech ◽  
...  
Keyword(s):  
Chronic Granulomatous Disease ◽  
Granulomatous Disease
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