Antibody-mediated cellular cytotoxicity against Raji cells ADCC(RAJI): Evaluation of false positives in the detection of circulating immune complexes by Raji-cell assay

1982 ◽  
Vol 2 (3) ◽  
pp. 197-204 ◽  
Author(s):  
Mrinal K. Dasgupta ◽  
Thavisakdi Kovithavongs ◽  
Joy Schlaut ◽  
Bryan M. Longenecker ◽  
John B. Dossetor
Keyword(s):  
Immune Complexes ◽  
Raji Cell ◽  
False Positives ◽  
Circulating Immune Complexes ◽  
Cellular Cytotoxicity ◽  
Raji Cells ◽  
Cell Assay

Feline complement dependent binding in the Raji cell assay is insufficient for the quantitation of immune complexes

1983 ◽  
Vol 5 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Darrell L. Tuomari ◽  
Richard G. Olsen ◽  
Larry E. Mathes ◽  
Eric H. Kraut
Keyword(s):  
Immune Complexes ◽  
Raji Cell ◽  
Cell Assay

scholarly journals BINDING OF SOLUBLE IMMUNE COMPLEXES TO HUMAN LYMPHOBLASTOID CELLS

1974 ◽  
Vol 140 (5) ◽  
pp. 1230-1244 ◽  
Author(s):  
Argyrios N. Theofilopoulos ◽  
Curtis B. Wilson ◽  
Viktor A. Bokisch ◽  
Frank J. Dixon
Keyword(s):  
Immune Complex ◽  
Immune Complexes ◽  
Gamma Globulin ◽  
Raji Cell ◽  
Lymphocytic Choriomeningitis ◽  
Human Igg ◽  
Raji Cells ◽  
Human Lymphoblastoid Cell ◽  
Human Gamma Globulin

Cells from a human lymphoblastoid cell line (Raji), with B-cell characteristics, and having receptors for human IgG Fc, C3b, and C3d, were used in an immunofluorescence test as in vitro detectors of immune complexes in animal and human sera. By this test, as little as 200–300 ng aggregated human gamma globulin or immune complexes per ml serum could be detected. The receptors for IgG Fc on the Raji cells were shown to be inefficient in detecting aggregated human gamma globulin and binding of aggregates to these receptors was inhibited by physiologic concentrations of 7S human IgG. Enhancement of aggregated human gamma globulin binding and binding of immune complexes formed in vitro to Raji cells was observed when the receptors for complement on these cells were used. By using the receptors for complement on Raji cells, circulating immune complexes were detected in rabbits with acute serum sickness, in mice with acute lymphocytic choriomeningitis virus infection, and in humans with immune complex type glomerulonephritis. The Raji cell test may be useful in detecting complement fixing immune complexes in different disease states, in monitoring circulating complexes in patients with immune complex diseases and in identifying the antigen(s) responsible for the induction of pathogenic immune complexes in humans and animals.

Detection of circulating immune complexes with a modified raji cell technique

1977 ◽  
Vol 55 (18) ◽  
pp. 899-901 ◽  
Author(s):  
T. H. H�tteroth ◽  
K. H. Meyer zum B�schenfelde
Keyword(s):  
Immune Complexes ◽  
Raji Cell ◽  
Circulating Immune Complexes

Serial Studies of Circulating Immune Complexes in Poststreptococcal Glomerulonephritis

PEDIATRICS ◽  
1982 ◽  
Vol 70 (5) ◽  
pp. 725-727
Author(s):  
Ching-Yuang Lin
Keyword(s):  
Acute Phase ◽  
Immune Complexes ◽  
Raji Cell ◽  
Circulating Immune Complexes ◽  
Initial Attack ◽  
Poststreptococcal Glomerulonephritis ◽  
Acute Poststreptococcal Glomerulonephritis ◽  
Radioimmune Assay ◽  
One Year

Levels of circulating immune complexes were determined in 30 children who suffered from acute poststreptococcal glomerulonephritis. The study was started in the acute phase of the disease and continued for one year. Raji cell radioimmune assay was used for the detection of immune complexes. The patients had significantly elevated levels of immune complexes during the acute phase, especially in the first three days after the onset of hematuria. Six months later the levels of immune complexes declined to slightly elevated levels, and nine months after the initial attack, no immune complexes were detectable. However, the patients who had persistent hematuria and proteinuria continued to have detectable immune complexes during this time.

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