Measurement of the diffusion of anisotropic particles in an external field by the method of correlation spectroscopy

1986 ◽  
Vol 29 (2) ◽  
pp. 141-143
Author(s):  
N. K. Nadirov ◽  
�. A. Manykin ◽  
S. M. Burkitbaev ◽  
M. I. Sharonov
Keyword(s):  
External Field ◽  
Correlation Spectroscopy ◽  
Anisotropic Particles ◽  
Method Of Correlation

A variant method of correlation spectroscopy

1987 ◽  
Vol 46 (5) ◽  
pp. 500-504
Author(s):  
�. L. Al'tman ◽  
A. I. Lopatin ◽  
Yu. P. Turunov
Keyword(s):  
Correlation Spectroscopy ◽  
Variant Method ◽  
Method Of Correlation

Physico-Chemical Properties of Recombinant Desulphatohirudin

1990 ◽  
Vol 63 (03) ◽  
pp. 499-504 ◽  
Author(s):  
A Electricwala ◽  
L Irons ◽  
R Wait ◽  
R J G Carr ◽  
R J Ling ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gel Filtration ◽  
Chemical Properties ◽  
Alpha Helix ◽  
Apparent Molecular Weight ◽  
Correlation Spectroscopy ◽  
Nmr Studies ◽  
Recombinant Hirudin ◽  
Physico Chemical ◽  
Recombinant Molecule

SummaryPhysico-chemical properties of recombinant desulphatohirudin expressed in yeast (CIBA GEIGY code No. CGP 39393) were reinvestigated. As previously reported for natural hirudin, the recombinant molecule exhibited abnormal behaviour by gel filtration with an apparent molecular weight greater than that based on the primary structure. However, molecular weight estimation by SDS gel electrophoresis, FAB-mass spectrometry and Photon Correlation Spectroscopy were in agreement with the theoretical molecular weight, with little suggestion of dimer or aggregate formation. Circular dichroism studies of the recombinant molecule show similar spectra at different pH values but are markedly different from that reported by Konno et al. (13) for a natural hirudin-variant. Our CD studies indicate the presence of about 60% beta sheet and the absence of alpha helix in the secondary structure of recombinant hirudin, in agreement with the conformation determined by NMR studies (17)

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