scholarly journals Induction of class II MHC antigens in vitro on pancreatic B cells isolated from BB/E rats

Diabetologia ◽  
1986 ◽  
Vol 29 (10) ◽  
pp. 749-751 ◽  
Author(s):  
R. Walker ◽  
A. Cooke ◽  
A. J. Bone ◽  
B. M. Dean ◽  
P. van der Meide ◽  
...  
Keyword(s):  
B Cells ◽  
Class Ii ◽  
Mhc Antigens ◽  
Class Ii Mhc ◽  
Class Ii Mhc Antigens ◽  
Pancreatic B Cells

scholarly journals Interleukin 10, a novel B cell stimulatory factor: unresponsiveness of X chromosome-linked immunodeficiency B cells.

1990 ◽  
Vol 172 (6) ◽  
pp. 1625-1631 ◽  
Author(s):  
N F Go ◽  
B E Castle ◽  
R Barrett ◽  
R Kastelein ◽  
W Dang ◽  
...  
Keyword(s):  
B Cells ◽  
Cell Viability ◽  
B Cell ◽  
X Chromosome ◽  
Class Ii ◽  
Interleukin 10 ◽  
Class I Mhc ◽  
Mhc Antigens ◽  
Class Ii Mhc ◽  
Class Ii Mhc Antigens

Highly purified, small dense splenic B cells from unstimulated mice showed increased expression of class II major histocompatibility complex (MHC) antigens and enhanced viability when cultured with affinity-purified recombinant interleukin 10 (rIL-10), compared with B cells cultured in medium alone. These responses were blocked by a monoclonal antibody (mAb) specific for IL-10, but not by an isotype-matched control antibody. IL-10 did not upregulate the expression of Fc epsilon receptors (CD23) or class I MHC antigens on small dense B cells or induce their replication as monitored by [3H]thymidine incorporation. While these B cell-stimulatory properties of IL-10 are also mediated by IL-4, the two cytokines appear to act independently in these assays; anti-IL-10 antibodies blocked IL-10 but not IL-4-mediated B cell viability enhancement, and vice versa. Similarly, since IL-4 upregulates CD23 on small dense B cells, the inability of IL-10 to do so argues against its acting via endogenously generated IL-4. Finally, IL-10 did not upregulate class II MHC antigens on B cells from X chromosome-linked immunodeficiency (XID) mice, while the same cells showed normal upregulation of class II antigens in response to IL-4. This report also extends our understanding of the relationship between IL-10 and the highly homologous Epstein-Barr virus (EBV)-encoded Bam HI fragment C rightward reading frame no. 1 (BCRFI) protein. It has previously been shown that BCRFI protein exhibits the cytokine synthesis inhibitory activity of IL-10. This report indicates that BCRFI protein also enhances in vitro B cell viability, but does not upregulate class II MHC antigens on B cells. One explanation for these data is that IL-10 contains at least two functional epitopes, only one of which has been conserved by EBV.

Expression of class II MHC antigens in murine pancreas after streptozocin-induced insulitis

Diabetes ◽  
1988 ◽  
Vol 37 (10) ◽  
pp. 1373-1379 ◽  
Author(s):  
A. G. Farr ◽  
J. W. Mannschreck ◽  
S. K. Anderson
Keyword(s):  
Class Ii ◽  
Mhc Antigens ◽  
Class Ii Mhc ◽  
Class Ii Mhc Antigens

scholarly journals Immunocytochemical identification of bovine Langerhans cells by use of a monoclonal antibody directed against class II MHC antigens.

1988 ◽  
Vol 36 (8) ◽  
pp. 991-995 ◽  
Author(s):  
L A Bryan ◽  
P J Griebel ◽  
D M Haines ◽  
W C Davis ◽  
J R Allen
Keyword(s):  
Monoclonal Antibody ◽  
Langerhans Cells ◽  
Staining Method ◽  
Class Ii ◽  
Immunocytochemical Staining ◽  
Microscopic Technique ◽  
Mhc Antigens ◽  
Class Ii Mhc ◽  
Class Ii Mhc Antigens ◽  
Class Ii Antigens

We undertook a study to develop a reliable light microscopic technique for identifying Langerhans cells (LC) in bovine epidermis. Monoclonal antibodies (MCA) detecting bovine class II MHC antigens were used in conjunction with an avidin-biotin-peroxidase complex (ABC) immunocytochemical staining method. The specificity of the MCA for LC was confirmed ultrastructurally by use of gold-labeled second antibody. Epidermal sheets and epidermal single-cell suspensions examined by light microscopy confirm that bovine epidermal LC express class II antigens. Anti-bovine class II MCA is a dependable reagent for identification of LC in normal bovine epidermis.

Class II MHC Antigens and Erythropoiesis

1986 ◽  
pp. 402-411
Author(s):  
B. Torok-Storb ◽  
F. W. Symington
Keyword(s):  
Class Ii ◽  
Mhc Antigens ◽  
Class Ii Mhc ◽  
Class Ii Mhc Antigens

scholarly journals Sequential loss of CD34 and class II MHC antigens on purified cord blood hematopoietic progenitors cultured with IL-3: characterization of CD34-, HLA-DR+ cells

Blood ◽  
1989 ◽  
Vol 74 (4) ◽  
pp. 1287-1294 ◽  
Author(s):  
C Caux ◽  
C Favre ◽  
S Saeland ◽  
V Duvert ◽  
P Mannoni ◽  
...  
Keyword(s):  
Cord Blood ◽  
Class Ii ◽  
Human Cord Blood ◽  
Mhc Antigens ◽  
Differentiated Cells ◽  
Hla Dr ◽  
Class Ii Mhc ◽  
Hla Dq ◽  
Class Ii Mhc Antigens ◽  
Cd34 Expression

Abstract The expression of class II MHC and CD34 antigens on human cord blood hematopoietic progenitor cells (HPC) was investigated upon culturing in the presence of interleukin-3 (IL-3). HPC isolated by “panning” according to their expression of CD34 coexpressed HLA-DR and HLA-DP, and the majority of the CD34+ HPC also expressed HLA-DQ. In the presence of IL-3, the expression of CD34 and class II MHC antigens was found to be gradually lost in culture. Loss of CD34 expression preceded loss of HLA-DR expression. After eight days of culture, CD34-, HLA-DR+ blast cells were obtained that strongly proliferated in response to IL- 3, GM-CSF, G-CSF, and M-CSF, and that had the capacity to generate macrophage and granulocyte colonies. After ten days of culture in IL-3, a population of CD34- cells that expressed low levels of HLA-DR (HLA- DRlo) was obtained by FACS-sorting. These CD34-, HLA-DRlo cells lacked colony-forming activity while the population expressing high levels of HLA-DR (HLA-DRhi) contained great numbers of colony-forming cells, and proliferated stronger in response to CSFs than the HLA-DRlo fraction. Finally CD34-, HLA-DR- cells that appeared later in the cultures (14 to 16 days) represented more differentiated cells with only marginal proliferative and no clonogenic capacity. These data indicate that whereas CD34 expression is associated with the multilineage potential of the HPC, HLA-DR expression correlates with overall proliferative capacity of hematopoietic cells during culture in IL-3.

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