Interaction of a phosphatidylcholine derivative of 1,6-diphenyl-1,3,5-hexatriene (DPH) with intact living cells: Steady-state fluorescence polarization and phase fluorometry studies

1993 ◽  
Vol 3 (4) ◽  
pp. 251-255 ◽  
Author(s):  
L. Miccoli ◽  
C. Szczepaniak ◽  
D. Dumas ◽  
S. Savonni�re ◽  
S. Muller ◽  
...  
Keyword(s):  
Steady State ◽  
Fluorescence Polarization ◽  
Living Cells ◽  
Phase Fluorometry ◽  
Steady State Fluorescence

scholarly journals Fluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report)

2013 ◽  
Vol 85 (3) ◽  
pp. 589-608 ◽  
Author(s):  
Marcel Ameloot ◽  
Martin vandeVen ◽  
A. Ulises Acuña ◽  
Bernard Valeur
Keyword(s):  
Steady State ◽  
Fluorescence Anisotropy ◽  
Fluorescence Polarization ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Steady State Fluorescence ◽  
Technical Report ◽  
Accuracy Of Measurements ◽  
Solution Methods ◽  
Systematic Effects

After recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample properties. A list of selected reference values of steady-state fluorescence anisotropy and polarization is given. Attention is also paid to analysis of time-resolved fluorescence anisotropy data obtained by pulse fluorometry or phase and modulation fluorometry techniques. Recommendations for checking the accuracy of measurements are provided together with a list of selected time-resolved fluorescence anisotropy data as reported in the literature.

Effect of growth temperature on the steady-state fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene incorporated into flight muscle mitochondrial membranes of houseflies (Musca domestica)

1984 ◽  
Vol 62 (9) ◽  
pp. 1747-1750
Author(s):  
R. Richard Plaskon ◽  
Gary L. Anderson
Keyword(s):  
Steady State ◽  
Musca Domestica ◽  
Growth Temperature ◽  
Fluorescence Polarization ◽  
Mitochondrial Membrane ◽  
Membrane Structure ◽  
Flight Muscle ◽  
Mitochondrial Membranes ◽  
Steady State Fluorescence ◽  
Degree Of Order

This study reports steady-state measurements of the fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) within flight muscle mitochondrial membrane fragments from adult houseflies (Musca domestica) grown at 15 and 25 °C. Growth temperature of the houseflies is shown to have no effect on the polarization of DPH's fluorescence, whereas the temperature sensitivity of fluorescence polarization (Δ polarization) is 16% higher in flies grown at 25 °C than in those grown at 15 °C. Since the fluorescence polarization of DPH gives an indication of the degree of order within membranes, these results suggest that houseflies have the ability to alter the temperature dependence of their flight muscle mitochondrial membrane structure in response to a change in temperature. Little attention has been directed to the effects of growth temperature on Δ polarization values and this study suggests a procedure for detecting the existence of changes in this quantity.

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