The effect of fat body on the differentiation in vitro of wing imaginal discs ofDrosophila melanogaster

1976 ◽  
Vol 180 (1) ◽  
pp. 73-77 ◽  
Author(s):  
Martin J. Milner ◽  
James H. Sang
Keyword(s):  
Fat Body ◽  
Imaginal Discs ◽  
Wing Imaginal Discs

scholarly journals Towards Long Term Cultivation of Drosophila Wing Imaginal Discs In Vitro

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e107333 ◽  
Author(s):  
Björn Handke ◽  
János Szabad ◽  
Peter V. Lidsky ◽  
Ernst Hafen ◽  
Christian F. Lehner
Keyword(s):  
Imaginal Discs ◽  
Drosophila Wing ◽  
Wing Imaginal Discs

The influence of hemolymph-binding protein on juvenile hormone stability and distribution in Manduca sexta fat body and imaginal discs in vitro

1975 ◽  
Vol 3 (3) ◽  
pp. 167-184 ◽  
Author(s):  
Bruce Hammock ◽  
Joachim Nowock ◽  
Walter Gooddman ◽  
Vassiliki Stamoudis ◽  
Lawrence I. Gilbert
Keyword(s):  
Juvenile Hormone ◽  
Manduca Sexta ◽  
Fat Body ◽  
Binding Protein ◽  
Imaginal Discs

Control of cuticle formation by wing imaginal discs in vitro

1979 ◽  
Vol 68 (2) ◽  
pp. 381-395 ◽  
Author(s):  
James B. Nardi ◽  
Judith H. Willis
Keyword(s):  
Imaginal Discs ◽  
Cuticle Formation ◽  
Wing Imaginal Discs

The eversion and differentiation of Drosophila melanogaster leg and wing imaginal discs cultured in vitro with an optimal concentration of β-ecdysone

Development ◽  
1977 ◽  
Vol 37 (1) ◽  
pp. 105-117
Author(s):  
Martin J. Milner
Keyword(s):  
Drosophila Melanogaster ◽  
Imaginal Discs ◽  
Optimal Concentration ◽  
Sensilla Trichodea ◽  
Wing Imaginal Discs ◽  
Wing Discs ◽  
Relationship Of ◽  
The Relationship

The stages of the eversion and differentiation of prothoracic leg and wing discs of Drosophila melanogaster, in Shields and Sang's medium 3, are described. The range of specific imaginal structures produced, including patterns of sensilla trichodea and sensilla campaniformia, are noted, and the relationship of these structures to those differentiated in situ is discussed.

scholarly journals Tick Importin-α Is Implicated in the Interactome and Regulome of the Cofactor Subolesin

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 457
Author(s):  
Sara Artigas-Jerónimo ◽  
Margarita Villar ◽  
Alejandro Cabezas-Cruz ◽  
Grégory Caignard ◽  
Damien Vitour ◽  
...  
Keyword(s):  
Rational Design ◽  
Fat Body ◽  
Animal Health ◽  
Direct Interaction ◽  
Vaccine Antigen ◽  
Tick Feeding ◽  
Interacting Protein ◽  
Expression Levels ◽  
Importin Α

Ticks and tick-borne diseases (TBDs) represent a burden for human and animal health worldwide. Currently, vaccines constitute the safest and most effective approach to control ticks and TBDs. Subolesin (SUB) has been identified as a vaccine antigen for the control of tick infestations and pathogen infection and transmission. The characterization of the molecular function of SUB and the identification of tick proteins interacting with SUB may provide the basis for the discovery of novel antigens and for the rational design of novel anti-tick vaccines. In the present study, we used the yeast two-hybrid system (Y2H) as an unbiased approach to identify tick SUB-interacting proteins in an Ixodes ricinus cDNA library, and studied the possible role of SUB as a chromatin remodeler through direct interaction with histones. The Y2H screening identified Importin-α as a potential SUB-interacting protein, which was confirmed in vitro in a protein pull-down assay. The sub gene expression levels in tick midgut and fat body were significantly higher in unfed than fed female ticks, however, the importin-α expression levels did not vary between unfed and fed ticks but tended to be higher in the ovary when compared to those in other organs. The effect of importin-α RNAi was characterized in I. ricinus under artificial feeding conditions. Both sub and importin-α gene knockdown was observed in all tick tissues and, while tick weight was significantly lower in sub RNAi-treated ticks than in controls, importin-α RNAi did not affect tick feeding or oviposition, suggesting that SUB is able to exert its function in the absence of Importin-α. Furthermore, SUB was shown to physically interact with histone 4, which was corroborated by protein pull-down and western blot analysis. These results confirm that by interacting with numerous tick proteins, SUB is a key cofactor of the tick interactome and regulome. Further studies are needed to elucidate the nature of the SUB-Importin-α interaction and the biological processes and functional implications that this interaction may have.

scholarly journals Oligomerization and endocytosis of Hedgehog is necessary for its efficient exovesicular secretion

2015 ◽  
Vol 26 (25) ◽  
pp. 4700-4717 ◽  
Author(s):  
Anup Parchure ◽  
Neha Vyas ◽  
Charles Ferguson ◽  
Robert G. Parton ◽  
Satyajit Mayor
Keyword(s):  
Cell Surface ◽  
Long Range ◽  
Imaginal Discs ◽  
Multivesicular Bodies ◽  
Selective Effect ◽  
Endosomal Sorting ◽  
Drosophila Wing ◽  
Dependent Process ◽  
Wing Imaginal Discs ◽  
Hh Signaling

Hedgehog (Hh) is a secreted morphogen involved in both short- and long-range signaling necessary for tissue patterning during development. It is unclear how this dually lipidated protein is transported over a long range in the aqueous milieu of interstitial spaces. We previously showed that the long-range signaling of Hh requires its oligomerization. Here we show that Hh is secreted in the form of exovesicles. These are derived by the endocytic delivery of cell surface Hh to multivesicular bodies (MVBs) via an endosomal sorting complex required for transport (ECSRT)–dependent process. Perturbations of ESCRT proteins have a selective effect on long-range Hh signaling in Drosophila wing imaginal discs. Of importance, oligomerization-defective Hh is inefficiently incorporated into exovesicles due to its poor endocytic delivery to MVBs. These results provide evidence that nanoscale organization of Hh regulates the secretion of Hh on ESCRT-derived exovesicles, which in turn act as a vehicle for long-range signaling.

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