Isolation of temperature sensitive mutations blocking clone development inDrosophila melanogaster, and the effects of a temperature sensitive cell lethal mutation on pattern formation in imaginal discs

1975 ◽  
Vol 178 (3) ◽  
pp. 247-275 ◽  
Author(s):  
Pat Simpson ◽  
Howard A. Schneiderman
Keyword(s):  
Pattern Formation ◽  
Imaginal Discs ◽  
Lethal Mutation ◽  
Temperature Sensitive ◽  
Sensitive Cell ◽  
Clone Development

scholarly journals A temperature-sensitive cell-cycle mutant of mammalian cells, tsJT16, is defective in a function operating soon after growth stimulation.

1990 ◽  
Vol 15 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Tsuyoshi Takasuka ◽  
Jun Ninomiya-Tsuji ◽  
Mihoko Sakayama ◽  
Sadahiko Ishibashi ◽  
Toshinori Ide
Keyword(s):  
Cell Cycle ◽  
Mammalian Cells ◽  
Growth Stimulation ◽  
Temperature Sensitive ◽  
Sensitive Cell

Transfer of a mutant viral thymidine kinase gene results in temperature-sensitive mouse cells

1983 ◽  
Vol 3 (3) ◽  
pp. 490-494
Author(s):  
M M Manos ◽  
S D Munroe ◽  
E Przybytkowska ◽  
L A McReynolds
Keyword(s):  
Cell Line ◽  
Thymidine Kinase ◽  
Growth Conditions ◽  
Selective Medium ◽  
Temperature Sensitive ◽  
Sensitive Cell ◽  
Kinase Gene ◽  
Vitro Analysis ◽  
Simplex Virus

Temperature-sensitive cell lines were obtained by DNA-mediated transfer of the thymidine kinase (TK) gene from a mutant, ts1117, of herpes simplex virus type 1. The cells died at 39 degrees C in selective medium which contained low levels (1 microgram/ml) of thymidine. In this lethal condition, no revertants were detected among 10(8) cells. It was shown by in vitro analysis of the TK activity that the temperature-sensitive cell line contains an enzyme whose activity is temperature sensitive and relatively unaffected by dTTP. The viral enzyme has these properties. The effect of the lethal growth conditions in the cell line was characterized by cell cycle analysis and rescue experiments which involved a shift to the permissive conditions. The successful transfer of the mutant viral TK activity to cells provides an additional selective marker for gene transfer.

The segment polarity gene armadillo interacts with the wingless signaling pathway in both embryonic and adult pattern formation

Development ◽  
1991 ◽  
Vol 111 (4) ◽  
pp. 1029-1043 ◽  
Author(s):  
M. Peifer ◽  
C. Rauskolb ◽  
M. Williams ◽  
B. Riggleman ◽  
E. Wieschaus
Keyword(s):  
Pattern Formation ◽  
Imaginal Discs ◽  
Protein Accumulation ◽  
Segment Polarity Genes ◽  
Adult Pattern ◽  
Segment Polarity ◽  
Lethal Allele ◽  
Segment Polarity Gene ◽  
Polarity Gene

The segment polarity genes of Drosophila were initially defined as genes required for pattern formation within each embryonic segment. Some of these genes also function to establish the pattern of the adult cuticle. We have examined the role of the armadillo (arm) gene in this latter process. We confirmed and extended earlier findings that arm and the segment polarity gene wingless are very similar in their effects on embryonic development. We next discuss the role of arm in pattern formation in the imaginal discs, as determined by using a pupal lethal allele, by analyzing clones of arm mutant tissue in imaginal discs, and by using a transposon carrying arm to produce adults with a reduced level of arm. Together, these experiments established that arm is required for the development of all imaginal discs. The requirement for arm varies along the dorsal-ventral and proximal-distal axes. Cells that require the highest levels of arm are those that express the wingless gene. Further, animals with reduced arm levels have phenotypes that resemble those of weak alleles of wingless. We present a description of the patterns of arm protein accumulation in imaginal discs. Finally, we discuss the implications of these results for the role of arm and wingless in pattern formation.

Studies on the female-sterile phenotype of 1(1)su(f) ts76a, a temperature-sensitive allele of the suppressor of forked mutation in Drosophila melanogaster

Development ◽  
1980 ◽  
Vol 55 (1) ◽  
pp. 247-256
Author(s):  
Thomas G. Wilson
Keyword(s):  
Cell Death ◽  
Drosophila Melanogaster ◽  
Follicle Cell ◽  
Lethal Mutation ◽  
Female Sterility ◽  
Temperature Sensitive ◽  
Egg Chambers ◽  
A Cell ◽  
Sensitive Allele

A new allele of the suppressor of forked [su(f)] mutation in Drosophila melanogaster has been found and designated 1(1)su(f)ts76a. It is temperature-sensitive for suppression of forked (f) and has additional temperature-sensitive phenotypes of lethality, female sterility, and abnormal bristle formation at 29 °C. It closely resembles two other conditional alleles of su(f), 1(1)su(f)ts67g and 1(1)ts726. Female sterility at 29 °C is characterized by both disorganized egg chambers in the ovarioles and also chorion-deficient oocytes. Both of these abnormalities may be the result of premature follicle cell death. The observations on 1(1)su(f)ts76a are consistent with the proposal that the similar allele, 1(1)ts726, is a cell-lethal mutation specifically affecting mitotically active cells.

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