Role of hydrophobic components of the plasma membrane in the response of frog urinary bladder cells to antidiuretic hormone

1987 ◽  
Vol 104 (5) ◽  
pp. 1505-1508
Author(s):  
Yu. V. Natochin ◽  
E. I. Shakhmatova ◽  
P. Bakoš
Keyword(s):  
Plasma Membrane ◽  
Urinary Bladder ◽  
Antidiuretic Hormone ◽  
Frog Urinary Bladder ◽  
Bladder Cells

scholarly journals Role of osmotic forces in exocytosis: studies of ADH-induced fusion in toad urinary bladder.

1981 ◽  
Vol 91 (2) ◽  
pp. 584-588 ◽  
Author(s):  
W A Kachadorian ◽  
J Muller ◽  
A Finkelstein
Keyword(s):  
Plasma Membrane ◽  
Urinary Bladder ◽  
Antidiuretic Hormone ◽  
Basic Mechanism ◽  
Toad Urinary Bladder ◽  
Bathing Medium ◽  
Intramembrane Particle ◽  
Luminal Membrane ◽  
Particle Aggregates

Antidiuretic hormone (ADH) treatment of toad urinary bladder activates an exocytotic-like process by which intramembrane particle aggregates are transferred from membranes of elongated cytoplasmic tubules to the luminal-facing plasma membrane. We find that the number of these ADH-induced fusion events, and the number of aggregates appearing in the luminal membrane, are reduced when the luminal bathing medium is made hyperosmotic. As an apparent consequence of the inhibition of their fusion with the luminal membrane, the elongated cytoplasmic tubules become enormously swollen into large, rounded vesicles. These results are consistent with the view that osmotic forces are essential to the basic mechanism of exocytosis.

The role of sodium-channel density in the natriferic response of the toad urinary bladder to an antidiuretic hormone

1982 ◽  
Vol 64 (1-2) ◽  
pp. 77-89 ◽  
Author(s):  
Jack H. Y. Li ◽  
Lawrence G. Palmer ◽  
Isidore S. Edelman ◽  
Bernd Lindemann
Keyword(s):  
Urinary Bladder ◽  
Sodium Channel ◽  
Antidiuretic Hormone ◽  
Toad Urinary Bladder ◽  
Channel Density

scholarly journals Role of prostaglandin E2 in regulation of low and high water osmotic permeability in frog urinary bladder

1997 ◽  
Vol 1356 (2) ◽  
pp. 160-170 ◽  
Author(s):  
Rimma G Parnova ◽  
Elena I Schakhmatova ◽  
Svetlana A Plesneva ◽  
Elena V Getmanova ◽  
Evgeny V Korolev ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Prostaglandin E2 ◽  
High Water ◽  
Frog Urinary Bladder ◽  
Osmotic Permeability

Inhibitory effect of phorbol ester on sodium transport in frog urinary bladder

1990 ◽  
Vol 259 (3) ◽  
pp. F425-F431
Author(s):  
T. Satoh ◽  
H. Endou
Keyword(s):  
Urinary Bladder ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Inhibitory Effect ◽  
Inhibitory Influence ◽  
Dependent Manner ◽  
Camp Accumulation ◽  
Frog Urinary Bladder ◽  
Na Transport

To confirm the role of protein kinase C (PKC) on epithelial Na transport, we studied the effects of phorbol 12-myristate 13-acetate (PMA) and dioctanoylglycerol (DiC8), activators of PKC, on short-circuit current (Isc) in frog urinary bladder and further examined the influence of sphingosine, an inhibitor of PKC, on PMA- or DiC8-modulated Isc. PMA reduced basal Isc in a dose-dependent manner, and sphingosine (10 and 100 microM) partially restored PMA-reduced Isc. On the other hand, DiC8 (5 x 10(-5) M) also reduced basal Isc, and this action was completely prevented by 100 microM sphingosine. Both PMA (4 x 10(-5) M) and DiC8 inhibited vasopressin (50 mU/ml)- and forskolin (5 x 10(-5) M)-stimulated increases in Isc. PMA (4 x 10(-5) M) also inhibited 8-bromoadenosine 3',5'-cyclic monophosphate (8-BrcAMP)-stimulated increase in Isc. Furthermore, PMA (4 x 10(-5) M) and DiC8 (5 x 10(-5) M) inhibited vasopressin (50 mU/ml)-stimulated cAMP accumulation. DiC8 also inhibited forskolin-stimulated cAMP accumulation. These results indicate that PMA exerts inhibitory influence on Na transport mainly by its own potency of PKC activation. In addition, it is suggested that there is a cross talk in epithelial Na transport between PKC and cAMP-dependent pathway in frog urinary bladder.

scholarly journals The Effect of Hypertonic Media on Water Permeability of Frog Urinary Bladder

1973 ◽  
Vol 61 (1) ◽  
pp. 110-124 ◽  
Author(s):  
P. Ripoche ◽  
J. Bourguet ◽  
M. Parisi
Keyword(s):  
Epithelial Cells ◽  
Urinary Bladder ◽  
Cyclic Amp ◽  
Antidiuretic Hormone ◽  
Water Permeability ◽  
Prostaglandin E1 ◽  
Physiological State ◽  
Solution Temperature ◽  
Bathing Solution ◽  
Frog Urinary Bladder

The frog urinary bladder undergoes, in some conditions, a marked increase of its water permeability when incubated in hypertonic media. This increase was observed with various nonpermeant solutes. It seems to result from the shrinkage of an osmo-sensitive compartment of the tissue, probably the epithelial cells. Many similarities were found between this effect and the physiological increase in water permeability (hydrosmotic response) elicited by antidiuretic hormone (ADH): both were dependent on the physiological state of the animals, and although the response was slower after hyperosmolar than after hormonal challenge, the patterns of response were similar, and in both cases markedly dependent on bathing solution temperature. Norepinephrine and prostaglandin E1, which in this tissue reduce the hydrosmotic action of ADH, presumably by inhibiting the adenyl cylase also reduced the effect of hyperosmolarity. Conversely this effect was potentiated by incubation in the presence of oxytocin, exogenous cyclic AMP, and theophylline, conditions in which the intracellular concentration of cyclic AMP is increased. These data demonstrate that the response to hyperosmolarity is elicited, at least partly, by mechanisms also involved in the physiological hydrosmotic response to ADH.

THE SWELLING OF FROG BLADDER CELLS PRODUCED BY OXYTOCIN

1965 ◽  
Vol 33 (2) ◽  
pp. 171-177 ◽  
Author(s):  
J. V. NATOCHIN ◽  
K. JANÁČEK ◽  
R. RYBOVÁ
Keyword(s):  
Urinary Bladder ◽  
Water Flow ◽  
Osmotic Gradient ◽  
Frog Urinary Bladder ◽  
Intracellular Space ◽  
Osmotic Water ◽  
Inulin Space ◽  
Bladder Cells ◽  
Synthetic Oxytocin ◽  
Osmotic Water Flow

SUMMARY (1) Synthetic oxytocin (100 m-u./ml.) produces swelling of the isolated frog urinary bladder even in the absence of an osmotic gradient across the bladder. (2) Calculations show that the change in intracellular space does not necessarily differ significantly from that in the presence of an osmotic gradient since the inulin space is markedly affected by the osmotic water flow. (3) Part of the cellular potassium is exchanged for sodium during the swelling produced by oxytocin. (4) A possible mechanism and the significance of the swelling is discussed.

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