Islet-cell aggregates: Structure and insulin-producing activity during in vitro culture

1990 ◽  
Vol 110 (6) ◽  
pp. 1718-1721
Author(s):  
V. G. Ablamunits ◽  
V. N. Blyumkin ◽  
G. M. Mogilevskii ◽  
L. A. Kirsanova ◽  
F. S. Baranova
Keyword(s):  
In Vitro Culture ◽  
Islet Cell ◽  
Cell Aggregates

Entrapment of Cultured Pancreas Islets in Three-Dimensional Collagen Matrices

1992 ◽  
Vol 1 (1) ◽  
pp. 51-60 ◽  
Author(s):  
Seh-Huang Chao ◽  
Madhusudan V. Peshwa ◽  
David E.R. Sutherland ◽  
Wei-Shou Hu
Keyword(s):  
Beta Cell ◽  
In Vitro Culture ◽  
Islets Of Langerhans ◽  
Single Cells ◽  
Three Dimensional ◽  
Dependent Manner ◽  
Cell Aggregates ◽  
Collagen Gels ◽  
Ideal System

In vitro culture of islets of Langerhans decreases their immunogenecity, presumably by eliminating passenger leukocytes and other Ia+ presenting cells within the islets. Islets cultivated in petri dishes either at 37°C or at 25°C gradually disintegrate during culture in a time-dependent manner which is related to the free-floating condition of the islets. Also, a fraction of the islets disperse as single cells and beta-cell aggregates or adhere to the bottom of the culture dishes. Thus, the retrieval rate of transplantable islets is dampened due to their disintegration and spontaneous dispersion in conventional petri dish cultures. Entrapment of freshly harvested islets of Langerhans in a three-dimensional collagen matrix was studied as an alternative method for islet cultivation. The contraction of collagen fibrils during in vitro culture counteracts the dispersion of islets and helps in maintaining their integrity while in culture. It was observed that the entrapped islets maintain satisfactory morphology, viability, and capability of glucose-dependent insulin secretion for over 2 wk. The oxygen consumption rate and glucose metabolism of these islets was not deranged when entrapped in collagen. Also, the retrieval of islets is easier and more efficient than that observed in conventional culture systems. Our results indicate that culture of islets in three-dimensional collagen gels can potentially develop into an ideal system applicable to clinical transplantation of cultured islets or beta-cell aggregates.

In vitro culture and labeling of neural cell aggregates followed by transplantation

1987 ◽  
Vol 98 (3) ◽  
pp. 606-615 ◽  
Author(s):  
Jorge A. Colombo ◽  
Jose I. Almeida ◽  
Santiago Molina
Keyword(s):  
In Vitro Culture ◽  
Neural Cell ◽  
Cell Aggregates

Isohexenylnaphthazarins from Lithospermum canescens (Michx.) Lehm. and Arnebia euchroma (Royle) Jonst. in vitro culture

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
K Graikou ◽  
H Damianakos ◽  
K Syklowska-Baranek ◽  
A Pietrosiuk ◽  
M Jeziorek ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Arnebia Euchroma ◽  
Lithospermum Canescens

Effect of antioxidants on in vitro culture of pomegranate cv. Sindhuri

2018 ◽  
Vol 34 (2) ◽  
pp. 311-318
Author(s):  
Ravi Kumar ◽  
◽  
M.L. Jakhar ◽  
Komal Sekhawat ◽  
Swarnlata Kumawat ◽  
...  
Keyword(s):  
In Vitro Culture

Leaf Color of Plants Regenerated through In Vitro Culture from Variegated Leaf Segments of Caladium.

1993 ◽  
Vol 62 (3) ◽  
pp. 619-624 ◽  
Author(s):  
Yu Zhu ◽  
Tetsuyuki Takemoto ◽  
Susumu Yazawa
Keyword(s):  
In Vitro Culture ◽  
Leaf Color ◽  
Leaf Segments ◽  
Variegated Leaf

In vitro Culture of Human PBMCs

BIO-PROTOCOL ◽  
2013 ◽  
Vol 3 (3) ◽  
Author(s):  
Santosh Panda ◽  
Balachandran Ravindran
Keyword(s):  
In Vitro Culture ◽  
Human Pbmcs

Morphogenesis of a Dissected Birch Leaf in vitro Culture

2020 ◽  
Vol 51 (6) ◽  
pp. 397-409
Author(s):  
O. S. Mashkina ◽  
T. M. Tabatskaya
Keyword(s):  
In Vitro Culture
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