Effect of ethaphon on the calcium ion concentration in smooth-muscle cells of the aorta

1991 ◽  
Vol 112 (2) ◽  
pp. 1100-1102
Author(s):  
P. V. Sergeev ◽  
A. S. Dukhanin ◽  
V. A. Nikolaevskii ◽  
K. M. Reznikov
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Calcium Ion ◽  
Muscle Cells ◽  
Ion Concentration ◽  
Calcium Ion Concentration

scholarly journals Spontaneous Oscillations of Cytoplasmic Free Calcium Ion Concentration in Cultured Smooth Muscle Cells from Guinea Pig Ileum

1993 ◽  
Vol 63 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Hisayuki Ohata ◽  
Toru Kawanishi ◽  
Megumi Kawanishi ◽  
Chikako Uneyama ◽  
Michihito Takahashi ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Calcium Ion ◽  
Muscle Cells ◽  
Ion Concentration ◽  
Free Calcium ◽  
Guinea Pig Ileum ◽  
Cultured Smooth Muscle Cells ◽  
Calcium Ion Concentration

scholarly journals Independent modes of propagation of calcium waves in smooth muscle cells

1993 ◽  
Vol 104 (4) ◽  
pp. 1101-1107 ◽  
Author(s):  
M.G. Mahoney ◽  
L.L. Slakey ◽  
P.K. Hepler ◽  
D.J. Gross
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Single Cells ◽  
Muscle Cells ◽  
Cytosolic Calcium ◽  
Temporal Organization ◽  
Ion Concentration ◽  
Calcium Waves ◽  
Multiple Loci ◽  
Calcium Ion Concentration

The purinergic agonist adenosine triphosphate (ATP) stimulates an initial transient followed by subsequent oscillations in cytosolic calcium ion concentration ([Ca2+]i) in individual porcine aortic smooth muscle cells. Using microinjection of fura-2 covalently coupled to dextran, we have analyzed in detail the spatial and temporal features of the oscillations. We have observed both cytoplasmic calcium waves and gradients within single cells. Single cells can contain multiple loci of initiation of oscillations. Independent oscillations in a single cell can have independent frequencies and these oscillations can propagate without interference across the same region of the cell, suggesting that they arise either from separately regulated stores of Ca2+ or a single Ca2+ store operated by two separate release mechanisms. The shape of the wave front and the manner of the waye's decay can vary from one oscillation to the next. Ca2+ signaling in individual arterial smooth muscle cells thus displays complex spatial and temporal organization.

Effect of calcium on neurohumoral stimulation of feline colonic smooth muscle

1982 ◽  
Vol 243 (2) ◽  
pp. G134-G140
Author(s):  
W. J. Snape
Keyword(s):  
Smooth Muscle ◽  
Spike Activity ◽  
Calcium Ion ◽  
Calcium Concentration ◽  
Extracellular Calcium ◽  
Ion Concentration ◽  
Myoelectric Activity ◽  
Ionic Calcium ◽  
Calcium Ion Concentration ◽  
Stimulation Of

The purpose of this study was to compare the effect of altering the extracellular calcium ion concentration on bethanechol or octapeptide of cholecystokinin (OP-CCK) stimulation of the isolated transverse colon of the cat. Myoelectric activity was recorded with monopolar glass-pore electrodes. Bethanechol (10(-6) M) stimulated an increase in the number of slow waves with superimposed spike potentials to 85.5 +/- 5.3% (P less than 0.001) compared with the basal spike activity (8.9 +/- 1.4%). OP-CCK (4 x 10(-9)) also increased spike activity (80.7 +/- 3.8%, P less than 0.001), which was not inhibited by atropine, phentolamine, or propranolol. Addition of 0.0 mM calcium solution to the colonic smooth muscle abolished both slow-wave and spike activity, which returned after replacing 0.25 mM calcium in the solution. Bethanechol stimulated a greater increase in spike activity as the concentration of calcium was increased. OP-CCK stimulation of colonic spike activity was more sensitive to the extracellular calcium concentration than bethanechol stimulation. Verapamil had a minimal effect on bethanechol stimulation of colonic spike activity, but it inhibited the OP-CCK stimulation. These studies suggest that 1) OP-CCK appears to stimulate colonic smooth muscle directly and 2) OP-CCK requires the presence of a greater amount of extracellular ionic calcium in order to stimulate colonic spike activity compared with bethanechol.

scholarly journals Ultrastructure of single smooth muscle cells contracted by carbachol and calcium ion.

1987 ◽  
Vol 10 (8) ◽  
pp. 396-403 ◽  
Author(s):  
IKUO MARUYAMA ◽  
MAMORU KOBAYASHI ◽  
CHIEKO YOSHIDA ◽  
KAZUTAKA MOMOSE
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Calcium Ion ◽  
Muscle Cells

Low [Mg2+]o induces contraction of cerebral arteries: roles of tyrosine and mitogen-activated protein kinases

2000 ◽  
Vol 279 (1) ◽  
pp. H185-H194 ◽  
Author(s):  
Zhi-Wei Yang ◽  
Jun Wang ◽  
Tao Zheng ◽  
Bella T. Altura ◽  
Burton M. Altura
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Sh2 Domain ◽  
Ion Concentration ◽  
Dependent Manner ◽  
Inhibitor Peptide ◽  
Mitogen Activated Protein ◽  
Basilar Arteries ◽  
Sb 203580

The present study was designed to investigate the mechanism of action of low extracellular magnesium ion concentration ([Mg2+]o) on isolated canine basilar arteries and single cerebral vascular smooth muscle cells from these arteries. Low-[Mg2+]o medium (0–0.6 mM) produces endothelium-independent contractions in isolated canine basilar arteries in a concentration-dependent manner; the lower the concentration of [Mg2+]o, the stronger the contractions. The low-[Mg2+]o medium-induced contractions are significantly attenuated by pretreatment of the arteries with low concentrations of either SB-203580, U-0126, PD-98059, genistein, or an Src homology 2 (SH2) domain inhibitor peptide. IC50 levels obtained for these five antagonists are consistent with reported inhibitor constant ( K i) values for these tyrosine kinase and mitogen-activated protein kinase (MAPK) antagonists. Low-[Mg2+]o medium (0–0.6 mM) produces transient intracellular calcium ion concentration ([Ca2+]i) peaks followed by a slow, sustained, and elevated plateau of [Ca2+]i in primary single smooth muscle cells from canine basilar arteries. Low-[Mg2+]o medium induces rapid and stable increases in [Ca2+]i; these increases are inhibited markedly in the presence of either SB-203580, U-0126, PD-98059, genistein or a SH2 domain inhibitor peptide. Several specific antagonists of known endogenously formed vasoconstrictors do not inhibit or attenuate either the low-[Mg2+]o-induced contractions or the elevation of [Ca2+]i. The present study suggests that activation of several cellular signaling pathways, such as protein tyrosine kinases (including the Src family) and MAPK, appears to play important roles in low-[Mg2+]o-induced contractions and the elevation of [Ca2+]i in smooth muscle cells from canine basilar arteries.

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