Effect of emoxypin on basal cyclic nucleotide phosphodiesterase activity and late receptor potential of the isolated retina

1989 ◽  
Vol 108 (3) ◽  
pp. 1248-1251
Author(s):  
A. A. Shvedova ◽  
N. B. Polyanskii ◽  
G. Kh. Akopyan ◽  
A. I. Dzhafarov
Keyword(s):  
Cyclic Nucleotide ◽  
Receptor Potential ◽  
Cyclic Nucleotide Phosphodiesterase ◽  
Phosphodiesterase Activity ◽  
Late Receptor Potential ◽  
Isolated Retina

Calmodulin-binding peptides isolated from α-casein peptone

1995 ◽  
Vol 62 (4) ◽  
pp. 587-592 ◽  
Author(s):  
Kenji Kizavva ◽  
Keiko Naganuma ◽  
Umeji Murakami
Keyword(s):  
Enzyme Activity ◽  
Cyclic Nucleotide ◽  
Cyclic Nucleotide Phosphodiesterase ◽  
Phosphodiesterase Activity ◽  
Calmodulin Binding ◽  
C Terminus ◽  
Binding Peptides

SUMMARYPeptides that inhibit calmodulin-dependent cyclic nucleotide phosphodiesterase were isolated from a pepsin digest of α-casein. Analysis of these peptides showed that they corresponded to the αs2-casein sequences 164–179 (Leu–Lys–Lys–Ile–Ser–Gln–Arg–Tyr–Gln–Lys–Phe–Ala–Leu–Pro–Gln–Tyr), 183–206 (Val–Tyr–Gln–His–Gln–Lys–Ala–Met–Lys–Pro–Trp–Ile–Gln–Pro–Lys–Thr–Lys–Val–Ile–Pro–Tyr–Val–Arg–Tyr) and 183–207 (C-terminus, Val–Tyr–Gln–His–Gln–Lys–Ala–Met–Lys–Pro–Trp–Ile–Gln–Pro–Lys–Thr–Lys–Val–Ile–Pro–Tyr–Val–Arg–Tyr–Leu). These peptides inhibited calmodulin-induced cyclic nucleotide phosphodiesterase activity over the range 1–50 μM without affecting the basal enzyme activity. These results demonstrated that the affinities of these peptides for calmodulin are comparable to the affinities of certain endogenous neurohormones and proteins that interact with calmodulin.

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