Improved efficiency for T-DNA-mediated transformation and plasmid rescue inArabidopsis thaliana

1993 ◽  
Vol 86 (5) ◽  
pp. 621-628 ◽  
Author(s):  
A. Mandal ◽  
V. L�ng ◽  
W. Orczyk ◽  
E. T. Palva
Keyword(s):  
Plasmid Rescue

scholarly journals Characterization of theAc/Ds behaviour in transgenic tomato plants using plasmid rescue

1992 ◽  
Vol 20 (1) ◽  
pp. 61-70 ◽  
Author(s):  
Caius M. T. Rommens ◽  
George N. Rudenko ◽  
Paul P. Dijkwel ◽  
Mark J. J. van Haaren ◽  
Pieter B. F. Ouwerkerk ◽  
...  
Keyword(s):  
Transgenic Tomato ◽  
Plasmid Rescue ◽  
Tomato Plants ◽  
Transgenic Tomato Plants

Isolation of the chicken thymidine kinase gene by plasmid rescue

Nature ◽  
1980 ◽  
Vol 285 (5762) ◽  
pp. 207-210 ◽  
Author(s):  
Manuel Perucho ◽  
Douglas Hanahan ◽  
Leah Lipsich ◽  
Michael Wigler
Keyword(s):  
Thymidine Kinase ◽  
Thymidine Kinase Gene ◽  
Plasmid Rescue ◽  
Kinase Gene

Recovery of DNA Sequences Flanking P-Element Insertions in Drosophila: Inverse PCR and Plasmid Rescue

2009 ◽  
Vol 2009 (4) ◽  
pp. pdb.prot5199-pdb.prot5199 ◽  
Author(s):  
A. M. Huang ◽  
E. J. Rehm ◽  
G. M. Rubin
Keyword(s):  
Dna Sequences ◽  
P Element ◽  
Inverse Pcr ◽  
Plasmid Rescue

A plasmid rescue technique for the recovery of plant DNA disrupted by T-DNA insertion

1992 ◽  
Vol 10 (2) ◽  
pp. 190-198 ◽  
Author(s):  
Friedrich J. Behringer ◽  
June I. Medford
Keyword(s):  
Plasmid Rescue ◽  
Plant Dna ◽  
Rescue Technique ◽  
Dna Insertion

Simplified plasmid rescue of host sequences adjacent to integrated proviruses

Gene ◽  
1996 ◽  
Vol 168 (2) ◽  
pp. 199-203 ◽  
Author(s):  
Buran Kurdi-Haidar ◽  
Theodore Friedmann
Keyword(s):  
Plasmid Rescue

Plasmid rescue — A tool for reproducible recovery of genes from transfected mammalian cells?

1984 ◽  
Vol 193 (3) ◽  
pp. 513-519 ◽  
Author(s):  
Udo Kiessling ◽  
Mathias Platzer ◽  
Michael Strauss
Keyword(s):  
Mammalian Cells ◽  
Plasmid Rescue

scholarly journals Analysis of Extrachromosomal Ac/Ds Transposable Elements

Genetics ◽  
2000 ◽  
Vol 155 (1) ◽  
pp. 349-359 ◽  
Author(s):  
Vera Gorbunova ◽  
Avraham A Levy
Keyword(s):  
Random Sequence ◽  
Donor Site ◽  
Plasmid Rescue ◽  
Present Evidence ◽  
Extrachromosomal Elements ◽  
Linear Elements ◽  
Ds Excision ◽  
Hairpin Formation ◽  
Insight Into ◽  
Original Donor

Abstract The mechanism of transposition of the maize Ac/Ds elements is not well understood. The true transposition intermediates are not known and it has not been possible to distinguish between excision models involving 8-bp staggered cuts or 1-bp staggered cuts followed by hairpin formation. In this work, we have analyzed extrachromosomal excision products to gain insight into the excision mechanism. Plasmid rescue was used to demonstrate that Ds excision is associated with the formation of circular molecules. In addition, we present evidence for the formation of linear extrachromosomal species during Ds excision. Sequences found at the termini of circular and linear elements showed a broad range of nucleotide additions or deletions, suggesting that these species are not true intermediates. Additional nucleotides adjacent to the termini in extrachromosomal elements were compared to the sequence of the original donor site. This analysis showed that: (1) the first nucleotide adjacent to the transposon end was significantly more similar to the first nucleotide flanking the element in the donor site than to a random sequence and (2) the second and farther nucleotides did not resemble the donor site. The implications of these findings for excision models are discussed.

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