Effect of the opioid peptide dalargin on repair processes during wound healing

1988 ◽  
Vol 106 (4) ◽  
pp. 1492-1495 ◽  
Author(s):  
A. B. Shekhter ◽  
A. I. Solov'eva ◽  
S. E. Spevak ◽  
M. I. Titov
Keyword(s):  
Wound Healing ◽  
Opioid Peptide ◽  
Repair Processes

scholarly journals The opioid peptide biphalin modulates human corneal epithelial wound healing in vitro

2019 ◽  
Author(s):  
Ozgun Melike Gedar Totuk ◽  
Erdost YILDIZ ◽  
Adriano MOLLICA ◽  
Kerem KABADAYI ◽  
Afsun SAHIN
Keyword(s):  
Wound Healing ◽  
Corneal Epithelium ◽  
Wound Closure ◽  
Opioid Peptide ◽  
Corneal Epithelial Cell ◽  
Scratch Assay ◽  
Corneal Epithelial ◽  
Significant Difference ◽  
Epithelial Wound Healing

Abstract Background Analgesic drugs including nonselective opioids and non-steroidal anti-inflammatory drugs should be used with great precautions to relieve the pain after physical damage of the corneal epithelium, because of their unfavorable effects on wound healing process. Biphalin, a synthetic opioid peptide, which has been demonstrated to possesses a strong analgesic effect on rodents. The purpose of this study is to investigate the effects of biphalin on human corneal epithelium wound healing. Methods Immortalized human corneal epithelial cell (HCEC) culture was used to test the effects of biphalin on wound healing. The toxicity of biphalin in various concentrations was measured with MTT assay. The effect of 1 µM and 10 µM biphalin were tested on wound closure at in vitro scratch assay of HCECs, and for cell migration and proliferation separately. Naloxone, a non-selective competitive antagonist of opioid receptor, was also used to inhibit the effects of biphalin in all experiments. Results Biphalin did not cause any toxic effect on HCECs in concentrations lower than 100 µM at various incubation time points. Biphalin increased wound closure process significantly at 1 µM concentration at in vitro scratch assay of HCECs (p < 0.05); also increased migration of HCECs significantly (p < 0.01). There was no significant difference between biphalin and control groups of HCECs at Ki67 proliferation assay. Conclusion Biphalin, a synthetic opioid peptide, has a potential role as a novel topical analgesic agent that promotes corneal epithelial wound healing.

Repair of Wounds of the Mucosa in the Rectum of the Cat

Development ◽  
1958 ◽  
Vol 6 (3) ◽  
pp. 509-517
Author(s):  
R. M. H. McMinn ◽  
F. R. Johnson
Keyword(s):  
Ulcerative Colitis ◽  
Wound Healing ◽  
Large Intestine ◽  
Ulcer Healing ◽  
Alimentary Canal ◽  
Intestinal Tract ◽  
Peptic Ulceration ◽  
Repair Processes ◽  
Series Of Experiments ◽  
Mucosal Lesions

Investigators of wound healing in the alimentary canal have carried out their experiments mainly in the gastro-duodenal region, in view of the importance of the problem of peptic ulceration in man. Little attention has been paid to repair processes at lower levels of the intestinal tract; histological investigations on this subject in the large intestine appear to have been carried out only by O'Connor (1954, 1956) and by Lumb & Protheroe (1955). Sircus (1956) studied the ulceration that ensued in portions of colon that had been implanted into the stomach wall in dogs, but his interest lay in the mechanism of ulcer production rather than ulcer healing, and Truelove's (1957) biopsies from patients with ulcerative colitis were used largely to assess a method of treatment and not to investigate repair processes. The present series of experiments was carried out in order to study the repair of mucosal lesions in the rectum of the cat.

A New in Vitro Method for the Measurement of Mechanical Strength of Abdominal Wounds in Laboratory Animals

1978 ◽  
Vol 7 (4) ◽  
pp. 217-221 ◽  
Author(s):  
R. van Noort ◽  
M. M. Black ◽  
M. G. Greaney ◽  
T. Irvin
Keyword(s):  
Wound Healing ◽  
Mechanical Strength ◽  
Quantitative Measurement ◽  
New Method ◽  
Laboratory Animals ◽  
Abdominal Wound ◽  
Testing Technique ◽  
In Vitro Method ◽  
Repair Processes

A new method for the measurement of abdominal wound strength in laboratory animals (rats) is described in detail. Preliminary experiments have been carried out and the results reported. These show the suitability of a diaphragm testing technique for measurement of wound strength in that it provides a more meaningful profile of the repair processes in abdominal wounds and makes possible the quantitative measurement of wound healing.

Involvement of KATPand KvLQT1 K+channels in EGF-stimulated alveolar epithelial cell repair processes

2007 ◽  
Vol 293 (4) ◽  
pp. L870-L882 ◽  
Author(s):  
Nguyen Thu Ngan Trinh ◽  
Anik Privé ◽  
Lina Kheir ◽  
Jean-Charles Bourret ◽  
Tiba Hijazi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Growth Factor ◽  
Alveolar Epithelial Cell ◽  
Alveolar Type ◽  
Growth Factor Signaling ◽  
Egf Receptors ◽  
Mechanical Wounding ◽  
Repair Processes ◽  
The Impact

Several respiratory diseases are associated with extensive damage of lung epithelia, and the regulatory mechanisms involved in their regeneration are not clearly defined. Growth factors released by epithelial cells or fibroblasts from injured lungs are important regulators of alveolar repair by stimulating cell motility, proliferation, and differentiation. In addition, K+channels regulate cell proliferation/migration and are coupled with growth factor signaling in several tissues. We decided to explore the hypothesis, never investigated before, that K+could play a prominent role in alveolar repair. We employed a model of mechanical wounding of rat alveolar type II epithelia, in primary culture, to study their response to injury. Wound healing was suppressed by one-half upon epidermal growth factor (EGF) titration with EGF-antibody (Ab) or erbB1/erbB2 tyrosine-kinase inhibition with AG-1478/AG-825. The addition of exogenous EGF slightly stimulated the alveolar wound healing and enhanced, by up to five times, alveolar cell migration measured in a Boyden-type chamber. Conditioned medium collected from injured alveolar monolayers also stimulated cell migration; this effect was abolished in the presence of EGF-Ab. The impact of K+channel modulators was examined in basal and EGF-stimulated conditions. Wound healing was stimulated by pinacidil, an ATP-dependent K+channel (KATP) activator, which also increased cell migration, by twofold, in basal conditions and potentiated the stimulatory effect of EGF. KATPor KvLQT1 inhibitors (glibenclamide, clofilium) reduced EGF-stimulated wound healing, cell migration, and proliferation. Finally, EGF stimulated KATPand KvLQT1 currents and channel expression. In summary, stimulation of K+channels through autocrine activation of EGF receptors could play a crucial role in lung epithelia repair processes.

scholarly journals The opioid peptide biphalin modulates human corneal epithelial wound healing in vitro

2020 ◽  
Author(s):  
Ozgun Melike Gedar Totuk ◽  
Erdost YILDIZ ◽  
Adriano MOLLICA ◽  
Kerem KABADAYI ◽  
Afsun SAHIN
Keyword(s):  
Wound Healing ◽  
Corneal Epithelium ◽  
Wound Closure ◽  
Opioid Peptide ◽  
Corneal Epithelial Cell ◽  
Scratch Assay ◽  
Corneal Epithelial ◽  
Significant Difference ◽  
Epithelial Wound Healing

Abstract Background: Analgesic drugs, including nonselective opioids and non-steroidal anti-inflammatory drugs, should be used with great precautions to relieve pain after physical damage of the corneal epithelium because of their unfavorable effects on the wound-healing process. Biphalin is a synthetic opioid peptide that has been demonstrated to possesses a strong analgesic effect on rodents. The purpose of this study is to investigate the effects of biphalin on human corneal epithelium wound healing.Methods: An immortalized human corneal epithelial cell (HCEC) culture was used to test the effects of biphalin on wound healing. The toxicity of biphalin in various concentrations was measured with the MTT assay. The effect of 1 µM and 10 µM biphalin were tested on wound closure in an in vitro scratch assay of HCECs and for cell migration and proliferation separately. Naloxone, a non-selective competitive antagonist of opioid receptors, was also used to inhibit the effects of biphalin in all experiments.Results: Biphalin did not cause any toxic effect on HCECs in concentrations lower than 100 µM at various incubation time points. Biphalin increased the wound closure process significantly at 1 µM concentration in an in vitro scratch assay of HCECs (p < 0.05). It also increased the migration of HCECs significantly (p < 0.01). There was no significant difference between biphalin and control groups of HCECs in the Ki67 proliferation assay.Conclusion: Biphalin, a synthetic opioid peptide, has a potential role as a novel topical analgesic agent that promotes corneal epithelial wound healing. This role should be evaluated in further in vivo and clinical studies.

scholarly journals In silico investigations on curcuminoids from Curcuma longa as positive regulators of the Wnt/β-catenin signaling pathway in wound healing

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Riyan Al Islam Reshad ◽  
Sayka Alam ◽  
Humaira Binte Raihan ◽  
Kamrun Nahar Meem ◽  
Fatima Rahman ◽  
...  
Keyword(s):  
Wound Healing ◽  
Binding Energy ◽  
Signaling Pathway ◽  
Binding Affinity ◽  
Curcuma Longa ◽  
Ligand Molecule ◽  
Casein Kinase 1 ◽  
Injury Repair ◽  
Repair Processes ◽  
Curcumin I

Abstract Background Curcuma longa (Turmeric) is a traditionally used herb in wound healing. The efficacy of fresh turmeric paste to heal wounds has already been investigated in multiple ethnobotanical studies. Wnt/β-catenin signaling pathway plays a significant role in wound healing and injury repair processes which has been evident in different in vitro studies. This study aims to analyze the potentiality of curcuminoids (curcumin I, curcumin II and curcumin III) from Curcuma longa to bind and enhance the activity of two intracellular signaling proteins- casein kinase-1 (CK1) and glycogen synthase kinase-3β (GSK3B) involved in Wnt/β-catenin signaling pathway. This study is largely based on a computer-based molecular docking program which mimics the in vivo condition and works on a specific algorithm to interpret the binding affinity and poses of a ligand molecule to a receptor. Subsequently, drug likeness property, ADME/Toxicity profile, pharmacological activity, and site of metabolism of the curcuminoids were also analyzed. Results Curcumin I showed better affinity of binding with CK1 (− 10.31 Kcal/mol binding energy) and curcumin II showed better binding affinity (− 7.55 Kcal/mol binding energy) for GSK3B. All of the ligand molecules showed quite similar pharmacological properties. Conclusion Curcumin has anti-oxidant, anti-carcinogenic, anti-mutagenic, anti-coagulant, and anti-infective properties. Curcumin has also anti-inflammatory and wound healing properties. It hastens wound healing by acting on different stages of the natural wound healing process. In this study, three curcumins from Curcuma longa were utilized in this experiment in a search for a drug to be used in wound healing and injury repair processes. Hopefully, this study will raise research interest among researchers.

scholarly journals Mitochondrial metabolism coordinates stage-specific repair processes in macrophages during wound healing

2021 ◽  
Author(s):  
Sebastian Willenborg ◽  
David E. Sanin ◽  
Alexander Jais ◽  
Xiaolei Ding ◽  
Thomas Ulas ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mitochondrial Metabolism ◽  
Repair Processes

Regulatory roles of androgens in cutaneous wound healing

2003 ◽  
Vol 90 (12) ◽  
pp. 978-985 ◽  
Author(s):  
Stephen Gilliver ◽  
Fred Wu ◽  
Gillian Ashcroft
Keyword(s):  
Wound Healing ◽  
Androgen Receptor ◽  
Tissue Repair ◽  
Systemic Treatment ◽  
Current Knowledge ◽  
Inflammatory Cells ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Repair Processes ◽  
Androgen Receptor Antagonist

SummaryAlthough the effects of androgens on wound healing are poorly characterised, the androgen receptor is expressed by inflammatory cells, keratinocytes and fibroblasts during wound healing, suggesting that androgens may regulate inflammatory and/or repair processes. In fact, it appears that endogenous testosterone inhibits wound healing and promotes inflammation since castration of male mice or systemic treatment with the androgen receptor antagonist flutamide accelerates cutaneous wound healing and reduces the inflammatory response. The aim of this review is to summarise our current knowledge about the regulation of tissue repair processes by androgens.

Alleviation of pain and depression by specific neural transplants: Fine structural correlates

1992 ◽  
Vol 50 (2) ◽  
pp. 1066-1067
Author(s):  
George D. Pappas ◽  
Jacqueline Sagen
Keyword(s):  
Opioid Peptides ◽  
Chromaffin Cells ◽  
Pain Sensitivity ◽  
Opioid Peptide ◽  
Local Source ◽  
Pain Models ◽  
Neural Transplants ◽  
Adrenergic Antagonists ◽  
Csf Levels ◽  
Donor Source

We have been interested in the use of neural transplants mainly as a local source of neuroactive substances, rather than as a replacement for damaged neural circuities. In particular, we have been exploring the possibilities of reducing pain by transplants of opioid peptide producing cells, and reducing depression by transplants of monoamine-producing cells. For the past several years, work in our laboratory has demonstrated in both acute and chronic pain models that transplantation of adrenal medullary tissue or isolated chromaffin cells into CNS pain modulatory regions can reduce pain sensitivity in rodents. Chromaffin cells were chosen as donor source since they produce high levels of both opioid peptides and catecholamines, substances which independently, and probably synergistically, reduce pain sensitivity when injected locally into the spinal cord. The analgesia produced by these transplants most likely results from the release of both opioid peptides and catecholamines, since it can be blocked or attenuated by opiate or adrenergic antagonists, respectively. Furthermore, CSF levels of met-enkephalin and catecholamines are increased by the transplants.

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