Effect of psychotropic drugs of the phenothiazine series on thymocyte DNA template activity and glucocorticoid-receptor interaction

1989 ◽  
Vol 107 (1) ◽  
pp. 66-69
Author(s):  
P. P. Golikov
Keyword(s):  
Glucocorticoid Receptor ◽  
Psychotropic Drugs ◽  
Receptor Interaction ◽  
Template Activity ◽  
Phenothiazine Series ◽  
Dna Template

scholarly journals A metal-linked gapped zipper model is proposed for theh sp90-glucocorticoid receptor interaction

FEBS Letters ◽  
1993 ◽  
Vol 315 (2) ◽  
pp. 109-113 ◽  
Author(s):  
J.A. Schwartz ◽  
H. Mizukami ◽  
D.F. Skafar
Keyword(s):  
Glucocorticoid Receptor ◽  
Receptor Interaction

The glucocorticoid receptor binds to a sequence overlapping the TATA box of the human osteocalcin promoter: a potential mechanism for negative regulation

1991 ◽  
Vol 11 (6) ◽  
pp. 3379-3383
Author(s):  
P E Strömstedt ◽  
L Poellinger ◽  
J A Gustafsson ◽  
J Carlstedt-Duke
Keyword(s):  
Glucocorticoid Receptor ◽  
Specific Binding ◽  
Tata Box ◽  
Receptor Interaction ◽  
Receptor Binding Site ◽  
Osteocalcin Gene ◽  
Close Proximity ◽  
Human Osteocalcin

Expression of the human osteocalcin promoter is negatively regulated by glucocorticoids in vivo. In vitro DNase I and exonuclease III footprinting analysis showed binding of purified glucocorticoid receptor in close proximity to and overlapping with the TATA box of the osteocalcin gene. These results imply competition or interference with binding of the TATA box-binding transcription factor IID as a mechanism of repression of this gene by glucocorticoids. In support of this notion, point mutation analysis of the receptor binding site indicated that flanking nucleotides and not the TATA box motif per se were important for receptor interaction. Moreover, DNA binding competition assays showed specific binding of the receptor only to the TATA box region of the osteocalcin gene and not to the corresponding region of an immunoglobulin heavy-chain promoter.

scholarly journals A Model of Glucocorticoid Receptor Interaction With Coregulators Predicts Transcriptional Regulation of Target Genes

2019 ◽  
Vol 10 ◽  
Author(s):  
Federico Monczor ◽  
Antonia Chatzopoulou ◽  
Carlos Daniel Zappia ◽  
René Houtman ◽  
Onno C. Meijer ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Glucocorticoid Receptor ◽  
Target Genes ◽  
Receptor Interaction

scholarly journals Glucocorticoid Receptor Interaction with 14-3-3 and Raf-1, a Proposed Mechanism for Cross-talk of Two Signal Transduction Pathways

2000 ◽  
Vol 275 (50) ◽  
pp. 39296-39301 ◽  
Author(s):  
Christina Widén ◽  
Johanna Zilliacus ◽  
Jan-Åke Gustafsson ◽  
Ann-Charlotte Wikström
Keyword(s):  
Signal Transduction ◽  
Glucocorticoid Receptor ◽  
Cross Talk ◽  
Receptor Interaction ◽  
Signal Transduction Pathways

scholarly journals Effects of polyamines and methylglyoxal bis(guanylhydrazone) on hepatic nuclear structure and deoxyribonucleic acid template activity

1975 ◽  
Vol 151 (3) ◽  
pp. 505-512 ◽  
Author(s):  
K B Brown ◽  
N F Nelson ◽  
D G Brown
Keyword(s):  
Dna Polymerase ◽  
Rat Liver ◽  
Template Activity ◽  
Isolated Rat Liver ◽  
E Coli ◽  
Rat Liver Nuclei ◽  
Calf Thymus ◽  
Liver Nuclei ◽  
Dna Template ◽  
Isolated Rat

1. The interaction of polyamines and methylglyoxal bis(guanythydrazone) (1, 1′-[(methylethanediylidene)-dinitrilo]diguanidine) with isolated rat liver nuclei was investigated by electron microscopy. 2. At 4mM, putrescine was without effect; however, spermidine, spermine or methylglyoxal bis(guanythydrazone) resulted in dispersed chromatin and alterations in nucleolar structure. In addition, spermidine or methylglyoxal bis(guanylhydrazone) caused marked aggregation of interchromatin granules. 3. The DNA template property of calf thymus DNA was examined by using DNA polymerases from Escherichia coli, Micrococcus lysodeikticus and calf thymus in the presence of 0-5 mM-amine. 4. In the presence of DNA polymerase, spermine or methylglyoxal bis(guanylhydrazone) inhibited activity, whereas putrescine or spermidine had much less effect or in some cases stimulated [3H]dTMP incorporation. 5. Template activity which was inhibited by spermine or methylglyoxal bis(guanylhydrazone) could be partially restored by additional DNA or enzyme. 6. When mixed with calf thymus DNA, calf thymus histone inhibited template activity as measured with E. coli DNA polymerase. The template activity of such a ‘histone-nucleate’ could not be restored by putrescine, spermidine, spermine or methylglyoxal bis(guanylhydrazone). 7. DNA template activity of isolated rat liver nuclei was tested by using E. coli DNA polymerase. None of the amines was able to increase the template activity of the nuclear DNA in vitro.

Effects of neurosteroids on glucocorticoid receptor-mediated gene transcription in LMCAT cells—A possible interaction with psychotropic drugs

2007 ◽  
Vol 17 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Agnieszka Basta-Kaim ◽  
Bogusława Budziszewska ◽  
Lucylla Jaworska-Feil ◽  
Monika Leśkiewicz ◽  
Magdalena Tetich ◽  
...  
Keyword(s):  
Glucocorticoid Receptor ◽  
Gene Transcription ◽  
Psychotropic Drugs
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