Ultracytochemical study of nuclear nucleoside phosphatase activity in epithelial cells of the normal human gastric mucosa and gastric carcinoma cells

1980 ◽  
Vol 90 (1) ◽  
pp. 965-969 ◽  
Author(s):  
I. B. Bukhvalov ◽  
A. G. Perevoshchikov ◽  
N. T. Raikhlin
Keyword(s):  
Gastric Mucosa ◽  
Epithelial Cells ◽  
Gastric Carcinoma ◽  
Phosphatase Activity ◽  
Carcinoma Cells ◽  
Human Gastric Mucosa ◽  
Normal Human

scholarly journals microRNA-29a-3p, Up-Regulated in Human Gastric Cells and Tissues with H.Pylori Infection, Promotes the Migration of GES-1 Cells via A20-Mediated EMT Pathway

2018 ◽  
Vol 51 (3) ◽  
pp. 1250-1263 ◽  
Author(s):  
Fengying Sun ◽  
Ying Ni ◽  
Hong Zhu ◽  
Jian Fang ◽  
Hua Wang ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Epithelial Cells ◽  
Western Blotting ◽  
Molecular Mechanisms ◽  
Luciferase Reporter ◽  
Human Gastric Mucosa ◽  
Gastric Epithelial Cells ◽  
Migration Ability ◽  
Gastric Cells ◽  
Dose Dependent

Background/Aims: Helicobacter pylori (H. pylori) infection is closely related to human gastric mucosa-associated diseases. Several recent studies on miRNAs have expanded our insights on H.pylori pathogenesis. This study aimed to investigate the biological roles and underlying molecular mechanisms of miR-29a-3p in human gastric cells and tissues with H.pylori infection. Methods: miR-29a-3p expression was quantified by quantitative RT-PCR (qRT-PCR). A miR-29a-3p target gene was validated by bioinformatics analysis, western blotting and dual luciferase reporter gene assays. Western blotting and immunohistochemistry (IHC) assay were performed to detect the protein expression. Transwell assay was used to determine the cell migration ability. Results: MiR-29a-3p was up-regulated in H.pylori-positive gastric mucosa tissues and H.pylori-infected gastric cells. The up-regulation of miR-29a-3p was dose-dependent in BGC-823 and GES-1 cells infected with H.pylori. Using gain- and loss-of-function experiments in vitro, we demonstrated that miR-29a-3p promoted the migration of gastric epithelial cells. We further characterized A20 as a direct target of miR-29a-3p. The expression of A20 was decreased in H.pylori-positive gastric mucosa tissues compared with H.pylori-negative gastric mucosa tissues. A20 downregulation was time- and dose-dependent in GES-1 and BGC-823 cells infected with H.pylori. In GES-1 and BGC-823 cells infected with H.pylori, the miR-29a-3p mimic significantly blocked A20 expression, which suggests that H.pylori decreased A20 expression through up-regulating miR-29a-3p in GES-1 and BGC-823 cells infected with H.pylori. The knockdown of A20 by siRNA enhanced the migration of human gastric epithelial cells and promoted the expression of Snail, Vimentin, and N-cadherin and inhibited the expression of E-cadherin. Conclusion: The miR-29a-3p may act as a tumor promotive miRNA by regulating cells migration through directly targeting of A20 gene in human gastric epithelial cells infected with H.pylori.

scholarly journals Isoenzymes of Lactate Dehydrogenase in Human Gastric Mucosa and Gastric Carcinoma Tissue

1966 ◽  
Vol 50 (6) ◽  
pp. 781-786 ◽  
Author(s):  
Peter E. Baume ◽  
Janet E. Builder ◽  
Barbara H. Fenton ◽  
Louise G. Irving ◽  
Douglas W. Piper
Keyword(s):  
Lactate Dehydrogenase ◽  
Gastric Mucosa ◽  
Gastric Carcinoma ◽  
Human Gastric Mucosa ◽  
Carcinoma Tissue ◽  
Gastric Carcinoma Tissue

O6-Alkyltransferase activity in normal human gastric mucosa

1990 ◽  
Vol 54 (3) ◽  
pp. 147-151 ◽  
Author(s):  
G.W. Dyke ◽  
J.L. Craven ◽  
R. Hall ◽  
D.P. Cooper ◽  
G. Soballa ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Human Gastric Mucosa ◽  
Normal Human

A gradient of TFF3 (trefoil factor family�3) peptide synthesis within the normal human gastric mucosa

2004 ◽  
Vol 316 (2) ◽  
pp. 155-165 ◽  
Author(s):  
Irina Kouznetsova ◽  
Ulrich Peitz ◽  
Michael Vieth ◽  
Frank Meyer ◽  
Else Marie Vestergaard ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Peptide Synthesis ◽  
Human Gastric Mucosa ◽  
Trefoil Factor ◽  
Normal Human ◽  
Trefoil Factor Family

Effect of Sucralfate on the Normal Human Gastric Mucosa. Endoscopic, Histologic, and Ultrastructural Assessment

1987 ◽  
Vol 22 (sup127) ◽  
pp. 111-123 ◽  
Author(s):  
Andrzej Taranwski ◽  
Daniel Hollander ◽  
Jerzy Stachure ◽  
Tomasz Mach ◽  
Jozef Bogdal
Keyword(s):  
Gastric Mucosa ◽  
Human Gastric Mucosa ◽  
Normal Human
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