Cell cycle and proliferative pool of human tumor strains transplanted in nude mice

1981 ◽  
Vol 92 (3) ◽  
pp. 1241-1243 ◽  
Author(s):  
E. S. Revazova ◽  
A. S. Petrova
Keyword(s):  
Cell Cycle ◽  
Nude Mice ◽  
Human Tumor ◽  
Proliferative Pool ◽  
Human Tumor Strains

Preparation of human tumor strains transplantable in nude mice and rats from cell lines

1985 ◽  
Vol 99 (4) ◽  
pp. 500-502
Author(s):  
E. S. Revazova ◽  
Yu. N. Solov'ev ◽  
T. M. Khizhnyakova ◽  
T. V. Yudicheva
Keyword(s):  
Cell Lines ◽  
Nude Mice ◽  
Human Tumor ◽  
Nude Mice And Rats ◽  
Human Tumor Strains

scholarly journals Resveratrol Modulation of Apoptosis and Cell Cycle Response to Cisplatin in Head and Neck Cancer Cell Lines

2021 ◽  
Vol 22 (12) ◽  
pp. 6322
Author(s):  
Marinela Bostan ◽  
Mirela Mihaila ◽  
Georgiana Gabriela Petrica-Matei ◽  
Nicoleta Radu ◽  
Razvan Hainarosie ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Head And Neck ◽  
Human Tumor ◽  
Tp53 Gene ◽  
Hacat Cells ◽  
Fadu Cells ◽  
Induced Apoptosis ◽  
Higher Doses ◽  
Harmful Effects

In head and neck cancers, the effectiveness of cisplatin (CisPt) treatment is limited by its toxicity, especially when higher doses are necessary, and the possible occurrence of cisplatin resistance. This study evaluated the effects of resveratrol (RSV) on the expression of different genes involved in the response of human tumor cells (FaDu, PE/CA-PJ49) to cisplatin therapy. Our results revealed that RSV induced apoptosis amplification in both FaDu and PE/CA-PJ49 cells and modulated the expression of specific genes differently than in normal HaCaT cells. In FaDu cells, combined CisPt + RSV treatment induced an increase in apoptosis, which was associated with an increase in c-MYC and TP53 and a decrease in BCL-2 expression. While CisPt + RSV treatment induced apoptosis in PE/CA-PJ49 cells by inhibition of BCL-2 associated with high levels of MDM-2 and subsequently led to inhibition of TP53 gene expression. Decreased c-MYC expression in PE/CA-PJ49 treated with CisPt + RSV was accompanied by cell cycle blockage in G0/G1 phase. In conclusion, RSV influences tumor cell response to CisPt by inducing apoptosis and modulating gene expression. In addition, in normal HaCaT cells, RSV was able to reduce the harmful effects of CisPt.

Hypothalamic appetite-regulating neuropeptide mRNA levels in cachectic nude mice bearing human tumor cells

Metabolism ◽  
2001 ◽  
Vol 50 (10) ◽  
pp. 1213-1219 ◽  
Author(s):  
N. Nara-Ashizawa ◽  
T. Tsukada ◽  
K. Maruyama ◽  
Y. Akiyama ◽  
N. Kajimura ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Nude Mice ◽  
Human Tumor ◽  
Mrna Levels ◽  
Human Tumor Cells

Nude mice with lacZ transduced human tumor xenografts as an in vivo model for invasion and metastasis

Lung Cancer ◽  
1991 ◽  
Vol 7 ◽  
pp. 30 ◽  
Author(s):  
Mogens Spang-Thomsen ◽  
James A. Zwiebel ◽  
Jørgen Rygaard ◽  
Nils Brünner
Keyword(s):  
Nude Mice ◽  
Human Tumor ◽  
In Vivo Model ◽  
Invasion And Metastasis ◽  
Human Tumor Xenografts ◽  
Tumor Xenografts

Survivin expression in correlation with apoptotic activity in canine lymphomas

2017 ◽  
Vol 20 (2) ◽  
pp. 329-338 ◽  
Author(s):  
J. Sokołowska ◽  
K. Urbańska
Keyword(s):  
Cell Cycle ◽  
Caspase 3 ◽  
Survivin Expression ◽  
Human Tumor ◽  
Apoptotic Index ◽  
Dual Function ◽  
Mean Values ◽  
Apoptotic Markers ◽  
Apoptotic Activity ◽  
Hodgkin's Lymphomas

AbstractSurvivin regulates cell cycle and mitosis and has antiapoptotic properties. Because of its dual function survivin has been the subject of much research focusing on its role in tumorigenesis and the relationship between survivin expression and apoptotic and/or proliferative activity in many types of human tumor including non-Hodgkin’s Lymphomas. Such studies have not been conducted in canine lymphomas. The aim of this study was to evaluate the expression of survivin in canine lymphomas of low (5/25) and high (20/25) grades in relation to apoptotic markers (apoptotic index and index of caspase-3). Survivin was found in all examined lymphomas. Most tumors (18/25) showed survivin expression in 10%-25% of positive cells. Only in single cases was lower (0-10% positive cells, 1/25) or higher (25%-50% and >50% positive cells, 5/25 and 1/25, respectively) survivin expression. No significant differences between mean values of either index of survivin or apoptotic index was found between low and high grade lymphomas. However, such a difference among lymphoma grades was shown regarding the caspase-3 index. No correlation between the survivin index and either the apoptotic index or caspase-3 index was found, irrespective of the method of quantification: in whole specimens or in areas of low and high survivin expression. Positive correlation was consistently noted only between both apoptotic markers. The results indicate that survivin is commonly expressed in canine lymphomas. It seems that survivin does not exhibit anti-apoptotic activity in canine lymphomas. Lack of correlation between survivin expression and apoptotic markers could indicate its potential role in cell cycle activation in lymphoma cells.

scholarly journals MCM7 Ubiquitination Regulates CMG Helicase Disassembly in Human

2021 ◽  
Author(s):  
Qianqian Sun ◽  
Kun Liu ◽  
Fangzhou Li ◽  
Bingquan Qiu ◽  
Zhisong Fu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Genome Stability ◽  
Human Tumor ◽  
Cell Fractionation ◽  
Minichromosome Maintenance ◽  
E3 Ligases ◽  
Minichromosome Maintenance Protein ◽  
Fork Stalling

Abstract BackgroundThe disassembly of the replisome plays an essential role in maintaining genome stability at the termination of DNA replication. However, the mechanism of replisome disassembly remains unknown in human. In this study, we screened E3 ligases and deubiquitinases (DUBs) for the ubiquitination of minichromosome maintenance protein (MCM) 7 and provided evidence of this process driving CMG helicase disassembly in human tumor cells. MethodsSILAC-MS/MS was analyzed to identify ubiquitinated proteins in HeLa cells. The ubiquitination/deubiquitylation assay in vitro and in vivo were detected by Western blot. Thymidine and HU were implied to synchronized cell cycle，and detect the role of ubiquitinated MCM7 in cell cycle. Cell fractionation assay was used to detect the function of ubiquitination of MCM7 in chromatin and non-chromatin. Aphidicolin、Etoposide、ICRF-193 and IR were applied to cause replication fork stalling. MG-132 and NMS-873 were used to inhibit the proteasome degradation and p97 segregase. Flow cytometer and FlowJo flow cytometry software were used to cell cycle analysis.ResultsIn our study, we found that the ubiquitin ligase RNF8 catalyzes the k63-linked poly-ubiquitination of MCM7 both in vivo and in vitro, and lysine 145 of MCM7 is the primary ubiquitination site. Moreover, the poly-ubiquitination of MCM7 mainly exists in the chromatin, which is dynamically regulated by the cell cycle, mainly occurs in the late S phase. And DNA damage can significantly reduce the poly-ubiquitylation of MCM7 in the late S phage. Furthermore, the proteasome, p97 segregase, USP29 and ATXN3 are required for the removal of MCM7 ubiquitination to promote the disassembly of CMG on chromatin. ConclusionsIn the late S phage of cell cycle, RNF8 catalyzes the poly-ubiquitination of MCM7, and then initiates the disassembly of CMG helicase from chromatin, which is mediated by p97, proteasome, USP29 and ATXN3 in human. We reveal the novel function of the poly-ubiquitylation of MCM7, which is a regulatory signal to control CMG complex unloading at replication termination sites.

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