Dynamics of DNA-dependent RNA synthesis in a cell-free system of Escherichia coli

1968 ◽  
Vol 65 (1) ◽  
pp. 47-50
Author(s):  
L. N. Kastrikina ◽  
V. S. Tongur
Keyword(s):  
Escherichia Coli ◽  
Rna Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals The Effect of Magnesium-Ion Concentration on the Translation of Phage-f2 RNA in a Cell-Free System of Escherichia coli

1972 ◽  
Vol 25 (2) ◽  
pp. 315-322 ◽  
Author(s):  
Wlodzimierz Zagorski ◽  
Witold Filipowicz ◽  
Aleksandra Wodnar ◽  
Andrzej Leonowicz ◽  
Ludwika Zagorska ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ion Concentration ◽  
Magnesium Ion ◽  
Free System ◽  
Cell Free System ◽  
A Cell

Effect of zeolites on protein synthesis in a cell-free system from Escherichia coli

1995 ◽  
Vol 17 (10) ◽  
pp. 1043-1046 ◽  
Author(s):  
Dong-Myung Kim ◽  
You-Eil Kim ◽  
Cha-Yong Choi
Keyword(s):  
Escherichia Coli ◽  
Protein Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals Hepatitis C Virus RNA Synthesis in a Cell-Free System Isolated from Replicon-Containing Hepatoma Cells

2003 ◽  
Vol 77 (3) ◽  
pp. 2029-2037 ◽  
Author(s):  
Richard W. Hardy ◽  
Joseph Marcotrigiano ◽  
Keril J. Blight ◽  
John E. Majors ◽  
Charles M. Rice
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rna Synthesis ◽  
De Novo ◽  
Hcv Rna ◽  
Free System ◽  
Cell Free System ◽  
Huh7 Cells ◽  
A Cell

ABSTRACT A number of hepatitis C virus (HCV) proteins, including NS5B, the RNA-dependent RNA polymerase, were detected in membrane fractions from Huh7 cells containing autonomously replicating HCV RNA replicons. These membrane fractions were used in a cell-free system for the analysis of HCV RNA replication. Initial characterization revealed a reaction in which the production of replicon RNA increased over time at temperatures ranging from 25 to 40°C. Heparin sensitivity and nucleotide starvation experiments suggested that de novo initiation was occurring in this system. Both Mn2+ and Mg2+ cations could be used in the reaction; however, concentrations of Mn2+ greater than 1 mM were inhibitory. Compounds shown to inhibit recombinant NS3 and NS5B activity in vitro were found to inhibit RNA synthesis in the cell-free system. This system should be useful for biochemical analysis of HCV RNA synthesis by a multisubunit membrane-associated replicase and for evaluating potential antiviral agents identified in biochemical or cell-based screens.

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