Investigations on the stability of bendamustin, a cytostatic agent of the nitrogen mustard type, I. Synthesis, isolation, and characterization of reference substances

1997 ◽  
Vol 128 (3) ◽  
pp. 291-299 ◽  
Author(s):  
R. Gust ◽  
R. Krauser
Keyword(s):  
Nitrogen Mustard ◽  
Type I ◽  
Cytostatic Agent ◽  
Isolation And Characterization ◽  
The Stability

scholarly journals Isolation and Characterization of Type I Signal Peptidase of Different Malaria Parasites

2005 ◽  
Vol 2005 (4) ◽  
pp. 301-309 ◽  
Author(s):  
Sutikshan Sharma ◽  
Arun Pradhan ◽  
Virander S. Chauhan ◽  
Renu Tuteja
Keyword(s):  
Signal Peptidase ◽  
Type I ◽  
Malaria Parasites ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of mitochondria and lysosome from isoproterenol induced cardiotoxic rats

2019 ◽  
Vol 8 (1) ◽  
pp. 12-18
Author(s):  
Malathi Mangalanathan ◽  
Tamiloli Devendhiran ◽  
Saraswathi Uthamaramasamy ◽  
Keerthika Kumarasamy ◽  
K Mohanraj ◽  
...  
Keyword(s):  
Heart Tissue ◽  
Cardiac Cells ◽  
Marker Enzymes ◽  
Membrane Function ◽  
Biochemical Alterations ◽  
Isolation And Characterization ◽  
The Stability ◽  
Membrane Pretreatment ◽  
Hydroethanolic Extract

Mitochondrial and lysosomal membranes are prominent membranes of cardiac cells and are the factors that determine membrane function in myocardial ischemia. In this study, isolation of mitochondria and lysosome from heart tissue under the control, isoproterenol (ISO) (8.5mg/100g) induced cardiotoxic rats and oral pretreatment with Z. armatum fruit (200, 400mg/kg body weight) treated rats. Further characterization of marker enzymes was done. A decreased in the activity of all the mitochondrial and lysosomal marker enzymes in ISO administered cardiotoxic rats when compared to control rats which indicate ISO decreased the stability of the membrane. Pretreatment with hydroethanolic extract of Z. armatum fruit to ISO induced rats significantly reverted these biochemical alterations near to normal. The possible mechanism for the protection of heart mitochondria and lysosome against oxidative damage induced by ISO might be due to quenching of free radicals and enhancing the action of marker enzymes.

scholarly journals Produksi, isolasi dan karakterisasi superoksida dismutase dari Spirulina platensis yang dibiakkan dalam serum lateks Production, isolation, and characterization of superoxyde dismutase from Spirulina platensis cultured on latex serum

2016 ◽  
Vol 77 (1) ◽  
Author(s):  
. TRI-PANJI ◽  
. SUHARYANTO ◽  
Marini WIJAYANTI
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Spray Drying ◽  
Spirulina Platensis ◽  
Active Agent ◽  
Pilot Scale ◽  
Isolation And Characterization ◽  
The Stability ◽  
Major Amino Acid

AbstractSpirulina platensis is a blue-green microalgawhich is frequently used for food and feedsupplements and cosmetic active agent. Thismicroalga also produces a strong antioxidantnamely superoxide dismutase (SOD) used ascosmetic active agent for anti aging and anti freeradicals. SOD was isolated from S. platensis cellbiomass from local isolate grown on latex serumon semipilot (3.5 m 3 ) and pilot scale (40 m 3 )then dried with spray drying or sun drying andcharacterized. SOD was purified with sequentialtwo-stage sedimentation using ammoniumsulphate and fractionated in chromatographiccolumn containing Sephadex G 200. Thefractions were analysed to determine the activity,cofactor metal and amino acid composition of theantioxidant. The results showed thatsedimentation of SOD extract with 80%ammonium sulphate produced SOD with higheractivity compared to that of SOD fromcommercial S. platensis biomass. This SOD wassuccessfully isolated and purified. MetaloenzymeSOD was composed of subunits with molecularweight of 77.78; 71.74; and 19.2 kDa, whichcontained nine types of amino acids with tyrosineand lysine as the major amino acid components.Zn was the most predominant metal on SOD, thenfollowed by Fe and Mn. The main subunitcofactors consisted of Zn 72%, Fe 25%, Mn 2%,and Cu 1%, which were different from thesmall subunit that contained of Zn 55%, Mn 31%,Fe 14%, and Cu 4%. The stability of SOD wasachieved on pH 7.5 and temperature below 25 o C.AbstrakSpirulina platensis adalah mikroalga hijaubiru yang banyak digunakan sebagai suplemenpangan, pakan, dan bahan aktif kosmetika.Mikroalga ini juga menghasilkan antioksidankuat yaitu superoksida dismutase (SOD), yangmerupakan bahan aktif kosmetika anti penuaandini dan pencegah efek radikal bebas. SODdiisolasi dari biomassa sel S. platensis isolat lokalyang dibiakkan dalam serum lateks skalasemipilot (3,5 m 3 ) dan pilot (40 m 3 ) sertadikeringkan dengan cara pengeringan kabut(spray drying) atau penjemuran untuk kemudiandikarakterisasi. SOD dimurnikan dengan peng-endapan bertingkat menggunakan ammoniumsulfat dan dipisahkan dengan kolom kromatografiberisi Sephadex G 200. Hasil pemisahankemudian dianalisis untuk menentukan aktivitas,logam kofaktor serta komposisi asam amino antioksidan tersebut. Hasil penelitian menunjukkanbahwa pengendapan ekstrak SOD denganSOD lebih tinggi dari SOD asal biomassaS. platensis komersial. SOD tersebut telahberhasil diisolasi dan dimurnikan. MetaloenzimSOD tersusun atas subunit dengan BM 77,78;71,74; dan 19,2 kDa, yang mengandungsembilan jenis asam amino dengan tirosin danlisin sebagai komponen asam amino utama.Logam yang dominan pada SOD adalah Zn,disusul kemudian Fe dan Mn. Kofaktor sub unitbesar terdiri dari Zn 72%, Fe 25%, Mn 2%, danCu 1%, berbeda dengan sub unit kecil yangmengandung Zn 55%, Mn 31%, Fe 14%, dan Cu4%. Stabilitas SOD S. platensis dicapai pada pH7,5 dan suhu di bawah 25 o Cammonium sulfat 80% menghasilkan aktivitas

scholarly journals Isolation and characterization of genomic DNA coding for α2 Type I collagen

1980 ◽  
Vol 8 (8) ◽  
pp. 1823-1837 ◽  
Author(s):  
G. Vogeli ◽  
E.V. Avvedimento ◽  
M. Sullivan ◽  
J.V. Maizel ◽  
G. Lozano ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Type I Collagen ◽  
Type I ◽  
Dna Coding ◽  
Isolation And Characterization

Isolation and characterization of drug delivering potential of type-I collagen from eel fish Evenchelys macrura

2012 ◽  
Vol 23 (7) ◽  
pp. 1729-1738 ◽  
Author(s):  
Anguchamy Veeruraj ◽  
Muthuvel Arumugam ◽  
Thangappan Ajithkumar ◽  
Thangavel Balasubramanian
Keyword(s):  
Type I Collagen ◽  
Type I ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of type I antifreeze proteins from cunner, Tautogolabrus adspersus, order Perciformes

FEBS Journal ◽  
2011 ◽  
Vol 278 (19) ◽  
pp. 3699-3710 ◽  
Author(s):  
Rod S. Hobbs ◽  
Margaret A. Shears ◽  
Laurie A. Graham ◽  
Peter L. Davies ◽  
Garth. L. Fletcher
Keyword(s):  
Antifreeze Proteins ◽  
Type I ◽  
Tautogolabrus Adspersus ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of novel primary cells from the human distal outflow pathway

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Uttio Roy Chowdhury ◽  
Cindy K. Bahler ◽  
Cheryl R. Hann ◽  
Bradley H. Holman ◽  
Michael P. Fautsch
Keyword(s):  
Trabecular Meshwork ◽  
Distal Region ◽  
Proximal Region ◽  
Cellular Growth ◽  
Model Systems ◽  
Type I ◽  
Isolation And Characterization ◽  
Outflow Pathway

AbstractOcular hypertension occurs due to increased resistance to aqueous humor removal through the conventional outflow pathway. Unlike the proximal region of the conventional outflow pathway, the distal region has not been well studied, mostly due to lack of model systems. Here we describe isolation and characterization of human primary vascular distal outflow pathway (VDOP) cells from the distal region of the conventional outflow pathway. Tissue from the distal region was isolated from human corneo-scleral rims, digested with collagenase type I (100 U/ml) and placed on gelatin coated plates to allow cellular growth in Dulbecco’s Modified Eagle’s Medium (low glucose) containing fetal bovine serum and antibiotic/antimycotic. VDOP cells showed consistent proliferation for up to 7 passages, retained endothelial-like nature of the parent tissues and showed a unique marker phenotype of Lectin+VEGFR2-CD34-NG2- that was distinct from neighboring trabecular meshwork (Lectin+VEGFR2-CD34-NG2+) and Schlemm’s canal (Lectin+VEGFR2+CD34+NG2+) cells. Dexamethasone treated VDOP cells did not express myocilin and did not form cross-linked actin networks, in contrast to trabecular meshwork cells. These data show that VDOP cells are unique to the distal outflow region and can be used as a viable in vitro model system to understand the biology of the distal outflow pathway and intraocular pressure regulation.

scholarly journals Produksi, isolasi dan karakterisasi superoksida dismutase dari Spirulina platensis yang dibiakkan dalam serum lateks Production, isolation, and characterization of superoxyde dismutase from Spirulina platensis cultured on latex serum

2016 ◽  
Vol 77 (1) ◽  
Author(s):  
. TRI-PANJI ◽  
. SUHARYANTO ◽  
Marini WIJAYANTI
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Spray Drying ◽  
Spirulina Platensis ◽  
Active Agent ◽  
Pilot Scale ◽  
Isolation And Characterization ◽  
The Stability ◽  
Major Amino Acid

AbstractSpirulina platensis is a blue-green microalgawhich is frequently used for food and feedsupplements and cosmetic active agent. Thismicroalga also produces a strong antioxidantnamely superoxide dismutase (SOD) used ascosmetic active agent for anti aging and anti freeradicals. SOD was isolated from S. platensis cellbiomass from local isolate grown on latex serumon semipilot (3.5 m 3 ) and pilot scale (40 m 3 )then dried with spray drying or sun drying andcharacterized. SOD was purified with sequentialtwo-stage sedimentation using ammoniumsulphate and fractionated in chromatographiccolumn containing Sephadex G 200. Thefractions were analysed to determine the activity,cofactor metal and amino acid composition of theantioxidant. The results showed thatsedimentation of SOD extract with 80%ammonium sulphate produced SOD with higheractivity compared to that of SOD fromcommercial S. platensis biomass. This SOD wassuccessfully isolated and purified. MetaloenzymeSOD was composed of subunits with molecularweight of 77.78; 71.74; and 19.2 kDa, whichcontained nine types of amino acids with tyrosineand lysine as the major amino acid components.Zn was the most predominant metal on SOD, thenfollowed by Fe and Mn. The main subunitcofactors consisted of Zn 72%, Fe 25%, Mn 2%,and Cu 1%, which were different from thesmall subunit that contained of Zn 55%, Mn 31%,Fe 14%, and Cu 4%. The stability of SOD wasachieved on pH 7.5 and temperature below 25 o C.AbstrakSpirulina platensis adalah mikroalga hijaubiru yang banyak digunakan sebagai suplemenpangan, pakan, dan bahan aktif kosmetika.Mikroalga ini juga menghasilkan antioksidankuat yaitu superoksida dismutase (SOD), yangmerupakan bahan aktif kosmetika anti penuaandini dan pencegah efek radikal bebas. SODdiisolasi dari biomassa sel S. platensis isolat lokalyang dibiakkan dalam serum lateks skalasemipilot (3,5 m 3 ) dan pilot (40 m 3 ) sertadikeringkan dengan cara pengeringan kabut(spray drying) atau penjemuran untuk kemudiandikarakterisasi. SOD dimurnikan dengan peng-endapan bertingkat menggunakan ammoniumsulfat dan dipisahkan dengan kolom kromatografiberisi Sephadex G 200. Hasil pemisahankemudian dianalisis untuk menentukan aktivitas,logam kofaktor serta komposisi asam amino antioksidan tersebut. Hasil penelitian menunjukkanbahwa pengendapan ekstrak SOD denganSOD lebih tinggi dari SOD asal biomassaS. platensis komersial. SOD tersebut telahberhasil diisolasi dan dimurnikan. MetaloenzimSOD tersusun atas subunit dengan BM 77,78;71,74; dan 19,2 kDa, yang mengandungsembilan jenis asam amino dengan tirosin danlisin sebagai komponen asam amino utama.Logam yang dominan pada SOD adalah Zn,disusul kemudian Fe dan Mn. Kofaktor sub unitbesar terdiri dari Zn 72%, Fe 25%, Mn 2%, danCu 1%, berbeda dengan sub unit kecil yangmengandung Zn 55%, Mn 31%, Fe 14%, dan Cu4%. Stabilitas SOD S. platensis dicapai pada pH7,5 dan suhu di bawah 25 o Cammonium sulfat 80% menghasilkan aktivitas

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