Comparative analysis of mitotic activity and chromosomal aberrations in cell lines contaminated by mycoplasmas and in decontaminated cell lines

1971 ◽  
Vol 72 (5) ◽  
pp. 1334-1336
Author(s):  
S. V. Kuz'mina ◽  
V. V. Neustroeva
Keyword(s):  
Comparative Analysis ◽  
Mitotic Activity ◽  
Cell Lines ◽  
Chromosomal Aberrations

scholarly journals Comparative analysis ofBRAF,NRASandc-KITmutation status between tumor tissues and autologous tumor cell-lines of stage III/IV melanoma

2014 ◽  
Vol 24 (1) ◽  
pp. 70-73 ◽  
Author(s):  
Anne-Chantal Knol ◽  
Marie-Christine Pandolfino ◽  
Audrey Vallée ◽  
Frédérique Nguyen ◽  
Virginie Lella ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Tumor Cell ◽  
Cell Lines ◽  
Stage Iii ◽  
Autologous Tumor Cell ◽  
Autologous Tumor ◽  
Tumor Cell Lines ◽  
Tumor Tissues

scholarly journals Comparative analysis of antibody- and lipid-based multiplexing methods for single-cell RNA-seq

2020 ◽  
Author(s):  
Viacheslav Mylka ◽  
Jeroen Aerts ◽  
Irina Matetovici ◽  
Suresh Poovathingal ◽  
Niels Vandamme ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Comparative Analysis ◽  
Single Cell ◽  
Cell Lines ◽  
Clinical Studies ◽  
Clinical Samples ◽  
Rna Seq ◽  
Batch Effects ◽  
Single Cell Sequencing ◽  
Single Nucleus

ABSTRACTMultiplexing of samples in single-cell RNA-seq studies allows significant reduction of experimental costs, straightforward identification of doublets, increased cell throughput, and reduction of sample-specific batch effects. Recently published multiplexing techniques using oligo-conjugated antibodies or - lipids allow barcoding sample-specific cells, a process called ‘hashing’. Here, we compare the hashing performance of TotalSeq-A and -C antibodies, custom synthesized lipids and MULTI-seq lipid hashes in four cell lines, both for single-cell RNA-seq and single-nucleus RNA-seq. Hashing efficiency was evaluated using the intrinsic genetic variation of the cell lines. Benchmarking of different hashing strategies and computational pipelines indicates that correct demultiplexing can be achieved with both lipid- and antibody-hashed human cells and nuclei, with MULTISeqDemux as the preferred demultiplexing function and antibody-based hashing as the most efficient protocol on cells. Antibody hashing was further evaluated on clinical samples using PBMCs from healthy and SARS-CoV-2 infected patients, where we demonstrate a more affordable approach for large single-cell sequencing clinical studies, while simultaneously reducing batch effects.

scholarly journals Comparative pathway enrichment analysis in gastrointestinal cell lines Caco-2, HT-29, HEPG2, and colon fibroblasts using a custom expression panel for tight-junction and cytoskeletal regulatory genes

2019 ◽  
Author(s):  
JM Robinson
Keyword(s):  
Gene Expression ◽  
Comparative Analysis ◽  
Tight Junction ◽  
Cell Lines ◽  
Enrichment Analysis ◽  
Pathway Enrichment Analysis ◽  
Intestinal Epithelial ◽  
Expression Data ◽  
Pathway Enrichment ◽  
Ht 29

AbstractThis brief report details results from a comparative analysis of Nanostring expression data between cell lines HEPG2, Caco-2, HT-29, and colon fibroblasts. Raw and normalized data are available publicly in the NCBI GEO/Bioproject databases. Results identify cell-line specific variations in gene expression relevant to intestinal epithelial function.

Chromosomal aberrations induced by 5-azacytidine combined with VP-16 (etoposide) in CHO-K1 and XRS-5 cell lines

2003 ◽  
Vol 23 (S1) ◽  
pp. 171-186 ◽  
Author(s):  
A.P.A. Guimar�es ◽  
F.L. Dias ◽  
R.S. Cardoso ◽  
S.N. Kronka ◽  
E.T. Sakamoto-Hojo
Keyword(s):  
Cell Lines ◽  
Chromosomal Aberrations

A NOVEL C1Q DOMAIN-CONTAINING PROTEIN ISOLATED FROM THE MOLLUSK MODIOLUS KURILENSIS RECOGNIZING GLYCANS ENRICHED WITH ACIDIC GALACTANS AND MANNANS IS VERY PROSPECTIVE FOR BIOMEDICAL PURPOSES

2021 ◽  
Vol 1 (19) ◽  
pp. 86-88
Author(s):  
A.V. Grinchenko ◽  
A. Kriegsheim ◽  
N.A. Shved ◽  
A.E. Egorova ◽  
D.V. Ilyaskina ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Comparative Analysis ◽  
Hela Cell ◽  
Cell Lines ◽  
Cancer Cell ◽  
Modiolus Kurilensis ◽  
Cell Phenotypes ◽  
Biomedical Potential

A novel C1qDC bivalve protein from the bivalve Modiolus kurilensis (MkC1qDC-1) was identified, isolated and characterized. MkC1qDC-1 demonstrated the inhibition of HeLa cell proliferation and recognition of some cancer cell phenotypes in comparative analysis on several cell lines, suggesting biomedical potential of this protein.

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