Effect of inhibitors of RNA and protein synthesis on the course of mitosis in a synchronized culture of Chinese hamster cells

1978 ◽  
Vol 85 (6) ◽  
pp. 810-814
Author(s):  
L. S. Strochkova
Keyword(s):  
Protein Synthesis ◽  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
Rna And Protein Synthesis ◽  
Effect Of Inhibitors

Protein synthesis and the presence or absence of a measurable G1 in cultured chinese hamster cells

1980 ◽  
Vol 104 (3) ◽  
pp. 461-467 ◽  
Author(s):  
R. Michael Liskay ◽  
Bruce Kornfeld ◽  
Paul Fullerton ◽  
Ronald Evans
Keyword(s):  
Protein Synthesis ◽  
Chinese Hamster ◽  
Chinese Hamster Cells

scholarly journals Chinese hamster cells can be reversibly blocked before mitosis with the protein synthesis inhibitor, emetine

1983 ◽  
Vol 59 (1) ◽  
pp. 257-268
Author(s):  
J.T. Westwood ◽  
E.B. Wagenaar
Keyword(s):  
Protein Synthesis ◽  
Mitotic Index ◽  
Chinese Hamster ◽  
Inhibitory Effect ◽  
Eukaryotic Cells ◽  
Protein Synthesis Inhibitor ◽  
Synthesis Inhibitor ◽  
Chinese Hamster Cells ◽  
Initial Concentration ◽  
G2 Phase

The inhibition of protein synthesis in eukaryotic cells will prevent them from entering mitosis. Emetine inhibits peptide elongation. When it was added to asynchronous populations of Chinese hamster ovary (CHO) cells, the mitotic index decreased sharply 30 to 40 min later. It was found that the inhibitory effect of emetine could be reversed when it was removed and the reversibility was dependent on both the initial concentration of emetine and the pH of the medium. Cell populations that were blocked by emetine for up to 2h showed a four- to fivefold increase in mitotic index approximately 1 h after the emetine was removed. These results indicate that there is a point or period in G2 phase at which critical ‘mitotic proteins’ are being synthesized, and if their synthesis is interrupted cells will fail to enter mitosis.

scholarly journals Temperature-Sensitive Chinese Hamster Fibroblast Mutant with a Defect in RNA Metabolism

1982 ◽  
Vol 2 (12) ◽  
pp. 1558-1573 ◽  
Author(s):  
Eric A. Wong ◽  
Immo E. Scheffler
Keyword(s):  
Protein Synthesis ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Temperature Sensitive ◽  
Chinese Hamster Fibroblast ◽  
Rna And Protein Synthesis ◽  
Shock Proteins ◽  
Short Time ◽  
Biosynthetic Machinery ◽  
Temperature Sensitive Phenotype

We describe a new temperature-sensitive mutant of Chinese hamster cell fibroblasts. After a shift to the nonpermissive temperature of 40.5°C, the rates of DNA, RNA, and protein synthesis declined rapidly (to ≤50% within 12 h) and the progression of unsynchronized cells through the cell cycle was affected. We believe that DNA synthesis came to a halt after a short time, because cells no longer entered the S phase. The decrease in protein synthesis at 40.5°C was shown to be a consequence of a decrease in the number of polysomes, whereas free 80S ribosomes accumulated. We concluded that the components of the protein biosynthetic machinery were intact (ribosomes and soluble factors), but synthesis was limited by a shortage of mRNA. The decline in mRNA production had a significant effect on the synthesis of proteins (e.g., heat shock proteins) translated from short-lived messages. We observed that both polyadenylated and nonpolyadenylated RNA syntheses declined at 40.5°C, whereas the synthesis of small RNAs (4 to 5S) was less reduced. The argument is made that the temperature-sensitive phenotype is the result of a defect affecting mRNA synthesis.

Temperature-Sensitive Chinese Hamster Fibroblast Mutant with a Defect in RNA Metabolism

1982 ◽  
Vol 2 (12) ◽  
pp. 1558-1573
Author(s):  
Eric A. Wong ◽  
Immo E. Scheffler
Keyword(s):  
Protein Synthesis ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Temperature Sensitive ◽  
Chinese Hamster Fibroblast ◽  
Rna And Protein Synthesis ◽  
Shock Proteins ◽  
Short Time ◽  
Biosynthetic Machinery ◽  
Temperature Sensitive Phenotype

We describe a new temperature-sensitive mutant of Chinese hamster cell fibroblasts. After a shift to the nonpermissive temperature of 40.5°C, the rates of DNA, RNA, and protein synthesis declined rapidly (to ≤50% within 12 h) and the progression of unsynchronized cells through the cell cycle was affected. We believe that DNA synthesis came to a halt after a short time, because cells no longer entered the S phase. The decrease in protein synthesis at 40.5°C was shown to be a consequence of a decrease in the number of polysomes, whereas free 80S ribosomes accumulated. We concluded that the components of the protein biosynthetic machinery were intact (ribosomes and soluble factors), but synthesis was limited by a shortage of mRNA. The decline in mRNA production had a significant effect on the synthesis of proteins (e.g., heat shock proteins) translated from short-lived messages. We observed that both polyadenylated and nonpolyadenylated RNA syntheses declined at 40.5°C, whereas the synthesis of small RNAs (4 to 5S) was less reduced. The argument is made that the temperature-sensitive phenotype is the result of a defect affecting mRNA synthesis.

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