Microthermal method of separating red blood cells into age groups

1977 ◽  
Vol 84 (3) ◽  
pp. 1372-1375
Author(s):  
N. A. Sizova ◽  
V. V. Kamenskaya ◽  
V. I. Fedenkov
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Age Groups

scholarly journals ELECTRON MICROSCOPE ANALYSIS OF YOUNG AND OLD RED BLOOD CELLS STAINED WITH COLLOIDAL IRON FOR SURFACE CHARGE EVALUATION

1969 ◽  
Vol 43 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Y. Marikovsky ◽  
D. Danon
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Membrane Surface ◽  
Phthalate Esters ◽  
Age Groups ◽  
Red Cells ◽  
Electric Mobility ◽  
Iron Particles ◽  
Colloidal Iron ◽  
Thin Sections

Human and rabbit red blood cells, separated into "young" and "old" age groups by differential flotation on phthalate esters, were fixed with glutaraldehyde and labeled with colloidal ferric oxide. Electron micrographs of thin sections of young cells showed a uniform and dense depostion of positive iron particles. Old cells showed particles deposited irregularly, leaving unlabeled gaps on the membrane surface. Red cells incubated with 10 units/ml receptor-destroying enzyme (RDE) demonstrate a reduced labeling, similar to that of old cells. After neuraminic acid had been removed from red cells by 20 units/ml RDE, no iron particles were found on membrane surfaces. The different labeling of young, old, and RDE-treated human and rabbit red cells was correlated with their electric mobility and agglutinability by poly-L-lysine. The contradiction between the apparent similarity in charge density of human and rabbit red cells as estimated by density of iron particles and the markedly lower electric mobility of rabbit red cells is discussed.

Differences in blood values depending on age in laboratory-bred european hamsters (Cricetus cricetus L.)

1975 ◽  
Vol 9 (1) ◽  
pp. 33-42 ◽  
Author(s):  
Adelheid Emminger ◽  
G. Reznik ◽  
Hildegard Reznik-Schüller ◽  
U. Mohr
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Age Groups ◽  
Blood Parameters ◽  
Cricetus Cricetus ◽  
Males And Females

No significant differences in blood parameters were found between males and females aged 13-900 days. A comparison of the various age groups showed significant differences in the number of erythrocytes, leucocytes, thrombocytes and in the diameter of red blood cells. The number of erythrocytes and leucocytes was lowest, and the erythrocytes had the largest diameters in young animals. The number of thrombocytes was lowest in older animals.

scholarly journals Continuous changes in biological levels of complete blood count in a high altitude area of China

2020 ◽  
Vol 8 ◽  
pp. 205031212093133 ◽  
Author(s):  
Xuan Luo ◽  
Lei Feng ◽  
XueJing Bai ◽  
JiangXian Zhu ◽  
GuanCheng Zhao ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Complete Blood Count ◽  
White Blood Cells ◽  
Age Groups ◽  
Reference Interval ◽  
Distribution Width ◽  
Men And Women ◽  
Cell Parameters

Objectives: We aimed to establish a new reference interval of blood cell parameters by classifying and counting blood Cells of 16- to 85-year-old healthy volunteers and observing continuous changes with age. Methods: We analyzed the blood cell parameters of 42,678 cases (men, 24,406; women, 18,272), and compared the blood cell parameters of men and women in different age groups using an independent samples t-test. Using limits of 2.5%–97.5%, a 90% confidence interval was used to develop new reference intervals. Results: Counts of blood cell parameters, including white blood Cells, neutrophils, lymphocytes, monocytes, eosinophils, basophils, red blood Cells, hemoglobin, hematocrit, distribution width of red blood Cells and platelets, were found to differ between men and women in different age groups. These parameters were used to establish a new reference interval of blood Cells. Conclusion: The blood cell parameters of both men and women changed with increasing age. The reference interval that we established will provide more accurate basic evidence for clinical diagnosis and treatment of diseases.

Interindividual variation in total and carrier-mediated lactate influx into red blood cells

1998 ◽  
Vol 274 (4) ◽  
pp. R1025-R1030 ◽  
Author(s):  
Leena K. Väihkönen ◽  
A. Reeta Pösö
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Venous Blood ◽  
Age Groups ◽  
Lactate Concentration ◽  
Bimodal Distribution ◽  
Monocarboxylate Transporter ◽  
Interindividual Variation ◽  
Transport Activity ◽  
Major Carrier

To study in standardbred horses interindividual variation in the influx of lactate into red blood cells, venous blood samples were collected from 89 horses from 2 wk to 9 yr of age. For 62 horses, the rate of influx was normally distributed with a mean rate of 4.09 nmol ⋅ mg protein−1 ⋅ min−1at a lactate concentration of 10 mM, and the respective value for the other 27 horses was 0.58 nmol ⋅ mg protein−1 ⋅ min−1. At 30 mM of lactate, the rates were 8.71 and 1.97 nmol ⋅ mg protein−1 ⋅ min−1, respectively. This bimodal distribution was independent of age. In horses with high transport activity, the monocarboxylate transporter (MCT) appears to be the major carrier, whereas, in those with low transport activity, no activity of the MCT could be detected. The band 3 protein may account for 18–39% of transport activity. With all age groups combined, the transport activity tended to be higher in mares than in stallions. Lactate transport into red blood cells seems thus to be an inherent property in which participation of various transporters varies interindividually.

scholarly journals Changes in erythrocytopoesis indices in dogs with babesiosis

2018 ◽  
Vol 9 (3) ◽  
pp. 379-383 ◽  
Author(s):  
V. I. Holovakha ◽  
О. V. Piddubnуak ◽  
T. I. Bakhur ◽  
N. V. Vovkotrub ◽  
A. A. Antipov ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Age Groups ◽  
Acid Resistance ◽  
Hypochromic Anemia ◽  
Common Disease ◽  
Babesia Canis ◽  
Age Composition ◽  
Hemoglobin Molecule ◽  
Total Tissue

Babesiosis is a common disease in dogs. Babesia canis (Piana & Galli-Valerio, 1895) (Sporozoa, Babesiidae) causes the destruction of erythrocytes, resulting in hypotensive shock and total tissue damage due to lack of oxygen. Because of babesiosis, anemia develops in dogs, and in the first hours of the disease it is normocytic, normochromic and nonregenerative, and on the 2–3rd day of the course, macrocytic, hypochromic anemia with reticulocytosis develops. Scientists have studied the most common indicators of evaluation of erythrocytopoesis during babesiosis (the number of red blood cells, hemoglobin, hematocrit index, indices of “red” blood MCH and MCV), but the age structure of erythrocytes, their acid resistance and the ferrum-transferrin complex for this parasitic pathology have not been sufficiently studied. We carried out research on dogs of service breeds, which were divided into two groups: the first (6–18 months old, n = 10) and the second (2–8 years, n = 15). According to the conducted studies, in dogs of different age groups with babesiosis revealed oligocythemia with anisocytosis and poikilocytosis, oligochromia, decreased hematocrit index and macrocytosis. Significant changes in the morpho-functional state of erythrocytes in dogs of both groups have been revealed, in particular, changes in the population (age) composition of red blood cells: the number of “old” erythrocytes increases (they are rapidly destroyed), the “young” forms of red blood cells decrease and the time for their hemolysis is reduced. As a result of the study of the ferrum-transferrin complex in dogs with babesiosis in both groups, an increase in the level of free ferrum (UIBC) and a decrease in the saturation of transferrin with the trace element was found, which makes it impossible to form a hemoglobin molecule in the bone marrow.

scholarly journals Mobility of Human Red Blood Cells of Different Age Groups in an Electric Field

Blood ◽  
1969 ◽  
Vol 33 (2) ◽  
pp. 159-163 ◽  
Author(s):  
A. YAARI
Keyword(s):  
Electric Field ◽  
Density Distribution ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Age Groups ◽  
Red Cells ◽  
Electric Mobility ◽  
Human Red Blood Cells ◽  
Healthy Donors ◽  
A Current

Abstract Using citrated blood of eleven healthy donors the density distribution of red blood cells (D.D.C.) was determined and eight fractions from each sample separated. Separated red cells were resuspended in their own plasma and employed for the electric mobility measurements. The Ruhenstroth-Bauer Cytopherometer was used at a current of 5 ma. at 24 C. The range of migration of red cells was found to be 0.855 to 1.47 x 10-4cm.2v-1sec.-1. In separated fractions the older the cells the slower they migrated in the electric field.

Measurement of nucleated red blood cells in the peripheral blood as a marker of hypoxia in sudden unexpected death in infancy

2015 ◽  
Vol 68 (9) ◽  
pp. 718-722 ◽  
Author(s):  
Rita E Zapata Vazquez ◽  
André Coetzee ◽  
Edward Harlock ◽  
Mark Simmerson ◽  
Marta C Cohen
Keyword(s):  
Red Blood Cells ◽  
Infant Death ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Age Groups ◽  
Sudden Infant Death ◽  
Sudden Infant ◽  
Unexpected Death ◽  
Nucleated Red Blood Cells ◽  
Scene Investigation

AimsA recently proposed classification of sudden unexpected infant death incorporates consideration of possibly asphyxia. This depends on an adequate postmortem, scene investigation and history. A reliable marker of asphyxia has yet to be identified. Such a marker could assist in classifying these deaths. Our aim was to determine if the level of nucleated red blood cells (nRBCs) in the peripheral blood could help identify those possibly asphyxia-related deaths and if risk factors could influence this level in the peripheral blood.MethodsCases of sudden unexpected deaths in infancy and sudden infant death syndrome (SIDS) which occurred over a period of 6 years (2007–2013) and were autopsied at Sheffield Children's Hospital were reviewed and categorised according to a new classification proposed by Randall et al. The cases were then correlated with the blood level of nRBCs determined at the time of post mortem examination. The study was approved by the Clinical Governance Department, number SE331.Results139 deaths were classified into Group A (true SIDS, 67 cases), Group B (possible asphyxia related, 24 cases), Group C (non-asphyxia-related, 6 cases), Group D (no crime scene investigation, 0 cases) and Group E (identifiable cause, 42 cases). The levels were significantly increased in ex-premature babies, in infants with an underlying condition (Group C) and in deaths related to illness or trauma (Group E). There was a trend towards higher levels of nRBCs in younger age groups and in babies born to smoking mothers.ConclusionsSIDS remains a difficult diagnosis to make despite the current medical technological advances where no marker of hypoxia has yet been identified.

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

Scanning electron microscopy of erythrophagocytosis by Entamoeba histolytica trophozoites

1992 ◽  
Vol 50 (1) ◽  
pp. 880-881
Author(s):  
Victor Tsutsumi ◽  
Adolfo Martinez-Palomo ◽  
Kyuichi Tanikawa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Red Blood Cells ◽  
Entamoeba Histolytica ◽  
Causative Agent ◽  
Blood Cells ◽  
Protozoan Parasite ◽  
Complex Phenomenon ◽  
Great Capacity ◽  
Scanning Electron

The protozoan parasite Entamoeba histolytica is the causative agent of amebiasis in man. The trophozoite or motile form is a highly dynamic and pleomorphic cell with a great capacity to destroy tissues. Moreover, the parasite has the singular ability to phagocytize a variety of different live or death cells. Phagocytosis of red blood cells by E. histolytica trophozoites is a complex phenomenon related with amebic pathogenicity and nutrition.

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