Diurnal changes in mitotic activity and glycogen content in rat liver during prolonged starvation

1968 ◽  
Vol 65 (2) ◽  
pp. 213-215 ◽  
Author(s):  
L. V. Sokolova
Keyword(s):  
Mitotic Activity ◽  
Rat Liver ◽  
Glycogen Content ◽  
Diurnal Changes ◽  
Prolonged Starvation

scholarly journals Postprandial Glycogen Content Is Increased in the Hepatocytes of Human and Rat Cirrhotic Liver

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 976
Author(s):  
Natalia N. Bezborodkina ◽  
Sergey V. Okovityi ◽  
Boris N. Kudryavtsev
Keyword(s):  
Rat Liver ◽  
Liver Tissue ◽  
Glycogen Content ◽  
Glycogen Synthase ◽  
Fibrous Tissue ◽  
Glycogen Metabolism ◽  
Liver Parenchyma ◽  
Dry Mass ◽  
Cirrhotic Liver ◽  
Liver Biopsies

Chronic hepatitises of various etiologies are widespread liver diseases in humans. Their final stage, liver cirrhosis (LC), is considered to be one of the main causes of hepatocellular carcinoma (HCC). About 80–90% of all HCC cases develop in LC patients, which suggests that cirrhotic conditions play a crucial role in the process of hepatocarcinogenesis. Carbohydrate metabolism in LC undergoes profound disturbances characterized by altered glycogen metabolism. Unfortunately, data on the glycogen content in LC are few and contradictory. In this study, the material was obtained from liver biopsies of patients with LC of viral and alcohol etiology and from the liver tissue of rats with CCl4-induced LC. The activity of glycogen phosphorylase (GP), glycogen synthase (GS), and glucose-6-phosphatase (G6Pase) was investigated in human and rat liver tissue by biochemical methods. Total glycogen and its labile and stable fractions were measured in isolated individual hepatocytes, using the cytofluorometry technique of PAS reaction in situ. The development of LC in human and rat liver was accompanied by an increase in fibrous tissue (20- and 8.8-fold), an increase in the dry mass of hepatocytes (by 25.6% and 23.7%), and a decrease in the number of hepatocytes (by 50% and 28%), respectively. The rearrangement of the liver parenchyma was combined with changes in glycogen metabolism. The present study showed a significant increase in the glycogen content in the hepatocytes of the human and the rat cirrhotic liver, by 255% and 210%, respectively. An increased glycogen content in cells of the cirrhotic liver can be explained by a decrease in glycogenolysis due to a decreased activity of G6Pase and GP.

scholarly journals Phospholipid metabolism during regeneration of rat liver

1970 ◽  
Vol 119 (4) ◽  
pp. 743-747 ◽  
Author(s):  
Göran Fex
Keyword(s):  
Mitotic Activity ◽  
Rat Liver ◽  
Phospholipid Metabolism ◽  
Liver Phospholipid ◽  
Regenerating Rat Liver

The concentration and composition of phospholipids and mitotic activity in regenerating rat liver were studied. (1) The total amount of liver phospholipid increased approximately linearly during 48h after operation but without change in the relative concentrations of individual phospholipids. (2) The appearance of mitoses 30h after operation was accompanied by an increased incorporation of 32P into the liver phospholipids. (3) The regenerating livers incorporated a higher percentage of the label into the phosphatidylserine+phosphatidylinositol fraction than those of control rats. The percentage of the label incorporated into phosphatidylethanolamine in these livers increased but decreased in the phosphatidylcholine.

Time Relations Among Metabolic and Morphologic 24-Hour Changes in Mouse Liver

1958 ◽  
Vol 195 (2) ◽  
pp. 301-310 ◽  
Author(s):  
Cyrus P. Barnum ◽  
Christine D. Jardetzky ◽  
Franz Halberg
Keyword(s):  
Mitotic Activity ◽  
Mouse Liver ◽  
Glycogen Content ◽  
Phase 1 ◽  
Liver Glycogen ◽  
Immature Mouse ◽  
Metabolic Variables ◽  
Cell Fractions ◽  
Cytoplasmic Rnas ◽  
Phase Differences

Phospholipid and RNA metabolism in immature mouse liver, in different cell fractions obtained by centrifugation, are 24-hour periodic. Twenty-four-hour rhythms also characterize the metabolism of DNA and mitotic activity. The established 24-hour rhythm in liver glycogen content stands out clearly under standardized circumstances of observation. Some peaks and troughs of these rhythms are dissociated in time. To the extent that radiotracer methods used herein indicate formation of the metabolic variables studied, it may be inferred that, ordinarily, in immature mouse liver, the peak formation of both phospholipid and RNA consistently leads in phase 1) peak DNA formation by 6–8 hours; 2) peak glycogen content by about 12 hours; and 3) peak mitotic activity by about 16 hours. Phase-differences among the curves describe a sequential order among certain metabolic and morphologic events at the intracellular level, as well as the time relations of these events. Since the curves for nuclear and cytoplasmic RNAs are more congruous than those for nuclear RNA and DNA metabolic rather than localized intracellular factors probably underlie this periodicity.

Small volume perfusion system of the isolated rat liver

1960 ◽  
Vol 15 (1) ◽  
pp. 128-132 ◽  
Author(s):  
Stanley D. Burton ◽  
Shirley St. George ◽  
Tadashi Ishida
Keyword(s):  
Rat Liver ◽  
Whole Blood ◽  
Technical Assistance ◽  
Glycogen Content ◽  
Hepatic Glycogen ◽  
Perfusion System ◽  
Isolated Rat Liver ◽  
Urea Production ◽  
Liver Changes ◽  
Isolated Rat

A perfusion system utilizing 25 ml of heparinized whole blood as a perfusate has been developed to study the metabolism of the isolated rat liver. Changes in the composition of the plasma of the perfusate were studied by circulation of the whole blood for 3 hours in the presence and absence of the liver. The discharge of glucose by the liver is marked by a pronounced initial hyperglycemia, which is countered slightly by red cell glycolysis. Despite continuous carbohydrate metabolism, a fall in sodium and a rise in potassium concentration occurs, ascribable chiefly to alteration of red blood cells. The hepatic glycogen content decreased slightly during perfusion. Urea production and bile flow were well preserved. Note: (With the Technical Assistance of Donna Cary and Veronica Mena) Submitted on June 26, 1959

GLYCOGEN CONTENT IN RAT LIVER. Importance for CT and MR imaging

2000 ◽  
Vol 41 (1) ◽  
pp. 92-96
Author(s):  
P. Leander ◽  
S. Mansson ◽  
G. Pettersson
Keyword(s):  
Mr Imaging ◽  
Rat Liver ◽  
Glycogen Content

Reduction of rat liver P450 enzyme function and glycogen content after induction of TNB-colitis

1998 ◽  
Vol 114 ◽  
pp. A1112-A1113
Author(s):  
H. Weidenbach ◽  
S. Leiz ◽  
M. Bachem ◽  
N. Dikopoulos ◽  
M. Reinshagen ◽  
...  
Keyword(s):  
Rat Liver ◽  
Glycogen Content ◽  
Enzyme Function ◽  
P450 Enzyme

Diurnal changes in mitotic activity and DNA synthesis in normal and tumor tissues of rats

1973 ◽  
Vol 76 (6) ◽  
pp. 1439-1441
Author(s):  
L. V. Sokolova ◽  
A. G. Mustafin ◽  
V. N. Dobrokhotov ◽  
S. I. Baluev
Keyword(s):  
Dna Synthesis ◽  
Mitotic Activity ◽  
Diurnal Changes ◽  
Tumor Tissues
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