Inhibition of migration of the blood leukocytes in guinea pigs with hypersensitivity of delayed type to foreign tissue antigen

1973 ◽  
Vol 76 (4) ◽  
pp. 1189-1192
Author(s):  
A. G. Artemova
Keyword(s):  
Guinea Pigs ◽  
Tissue Antigen ◽  
Hypersensitivity Of Delayed Type ◽  
Blood Leukocytes

Protection of lethally irradiated guinea pigs with fresh and frozen homologous peripheral blood leukocytes

Cryobiology ◽  
1965 ◽  
Vol 1 (3) ◽  
pp. 233-239 ◽  
Author(s):  
Vernon P. Perry ◽  
Theodore I. Malinin ◽  
Carey C. Kerby ◽  
Michael F. Dolan
Keyword(s):  
Peripheral Blood ◽  
Guinea Pigs ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes

scholarly journals Peripheral Leukocyte Infusion into Lethally Irradiated Guinea Pigs

Blood ◽  
1965 ◽  
Vol 25 (5) ◽  
pp. 693-702 ◽  
Author(s):  
THEODORE I. MALININ ◽  
VERNON P. PERRY ◽  
CAREY C. KERBY ◽  
MICHAEL F. DOLAN
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Guinea Pigs ◽  
Histologic Examination ◽  
Rapid Recovery ◽  
Lymphatic Tissue ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Peripheral Leukocyte ◽  
Secondary Disease

Abstract Homologous peripheral blood leukocytes were injected into lethally irradiated guinea pigs. Histologic examination of these showed a rapid recovery of the bone marrow and the lymphatic tissue following an injection of a sufficient number of leukocytes. All control animals died with a hypoplastic bone marrow. Protected animals did not develop "secondary" disease during 9 months of observation.

scholarly journals Study of the sensitizing effect of detergents intended for the care of dishes and other products for children

2021 ◽  
pp. 60-65
Author(s):  
A.M. Holichenkov ◽  
◽  
Yu.S. Domaratska ◽  
L.Yu. Hryhorenko ◽  
S.V. Stepanchuk ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Guinea Pigs ◽  
Tissue Antigen ◽  
Experimental Animals ◽  
Foreign Products ◽  
Sensitizing Effect ◽  
The Impact

Objective: We evaluated the sensitizing effect of various detergents intended for the care of dishes and other products for children. Materials and methods: The study was carried out in guinea pigs. The sensitization of the animal’s organism was studied in the reaction of degranulation of peripheral blood basophils (after Shelley). Forthestudy, we selected 10% solutions of domestic and foreign products for washing children's dishes ("Alionka", "Karapuz", "Ushastyi Nian", "Frosch Baby") purchased in the supermarkets of Kyiv. Results: In 21 days, the performed study made it possible to establish the development of sensitization of the organism of the animals in the experimental groups exposed to the solutions of the detergents "Alionka", "Karapuz", "Ushasty Nian” for washing children's dishes, although the degree of its manifestation was different. The solution of the detergent "Ushastyi Nian" was shown to possess more pronounced sensitizing properties, as evidenced by the development of weak positive autosensitization and positive sensitization of the organism of the experimental animals. The impact of the detergents for cleaning children's products "Alionka" and "Karapuz"on the animals’ organism leads to the development of mild sensitization, and when using the solution of "Alionka" detergent, a development of the weakly expressed autosensitization of the organism is also observed in the animals. There was no increase in the degranulation of basophilic granulocytes in the presence of both tissue antigen and hapten in the group of animals where the solution of detergent "Frosh Baby" was used, which indicated the absence of auto- and sensitization of the organism of the animals of this group.

Castaneda, M. Study of the mechanism of immunity in typhus. III. Detection of opsonins for Provacek's rickettsiae in immune typhus serum. (J. of Imunology, V. 3, No. 3, 1936)

1937 ◽  
Vol 33 (4) ◽  
pp. 515-515
Keyword(s):  
Guinea Pigs ◽  
Normal Blood ◽  
Blood Leukocytes

Back in 1922, Epstein discovered the phagocytosis of Provachek's rickettsiae in a mixture of typhus serum and normal blood leukocytes. These observations were not confirmed, however, other researchers also pointed to the presence of opsonic properties in the serum of typhus humans and guinea pigs.

Investigation of the relaxant effects of propofol on ovalbumin-induced asthma in guinea pigs

2006 ◽  
pp. 1
Author(s):  
I. Bagcivan ◽  
O. Cevit ◽  
M. K. Yildirim ◽  
S. Gursoy ◽  
S. Yildirim ◽  
...  
Keyword(s):  
Guinea Pigs

Hepatocyte Alterations in Guinea Pigs FED Polychlorinated Biphenyls (PCBs), Dibenzodioxins (PCDD), AND DIBENZOFURANS (PCDF)

1982 ◽  
Vol 40 ◽  
pp. 56-57
Author(s):  
J. N. Turner ◽  
D. N. Collins
Keyword(s):  
Guinea Pigs ◽  
Room Temperature ◽  
Dose Group ◽  
Methyl Cellulose ◽  
Uranyl Acetate ◽  
Target Organ ◽  
Office Building ◽  
Lead Citrate ◽  
Control Groups ◽  
Cooling Fluid

A fire involving an electric service transformer and its cooling fluid, a mixture of PCBs and chlorinated benzenes, contaminated an office building with a fine soot. Chemical analysis showed PCDDs and PCDFs including the highly toxic tetra isomers. Guinea pigs were chosen as an experimental animal to test the soot's toxicity because of their sensitivity to these compounds, and the liver was examined because it is a target organ. The soot was suspended in 0.75% methyl cellulose and administered in a single dose by gavage at levels of 1,10,100, and 500mgm soot/kgm body weight. Each dose group was composed of 6 males and 6 females. Control groups included 12 (6 male, 6 female) animals fed activated carbon in methyl cellulose, 6 males fed methyl cellulose, and 16 males and 10 females untreated. The guinea pigs were sacrificed at 42 days by suffocation in CO2. Liver samples were immediately immersed and minced in 2% gluteraldehyde in cacadylate buffer at pH 7.4 and 4°C. After overnight fixation, samples were postfixed in 1% OsO4 in cacodylate for 1 hr at room temperature, embedded in epon, sectioned and stained with uranyl acetate and lead citrate.

Glycogen in guinea pig neutrophils: Ultrastructural study of neutrophils at the intradermal site of BCG injection

1983 ◽  
Vol 41 ◽  
pp. 726-727
Author(s):  
Corazon D. Bucana
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Electron Density ◽  
Peritoneal Cavity ◽  
Ultrastructural Study ◽  
Guinea Pigs ◽  
Random Distribution ◽  
Glycogen Content ◽  
Polymorphonuclear Neutrophils ◽  
Ultrastructural Studies

In the circulating blood of man and guinea pigs, glycogen occurs primarily in polymorphonuclear neutrophils and platelets. The amount of glycogen in neutrophils increases with time after the cells leave the bone marrow, and the distribution of glycogen in neutrophils changes from an apparently random distribution to large clumps when these cells move out of the circulation to the site of inflammation in the peritoneal cavity. The objective of this study was to further investigate changes in glycogen content and distribution in neutrophils. I chose an intradermal site because it allows study of neutrophils at various stages of extravasation.Initially, osmium ferrocyanide and osmium ferricyanide were used to fix glycogen in the neutrophils for ultrastructural studies. My findings confirmed previous reports that showed that glycogen is well preserved by both these fixatives and that osmium ferricyanide protects glycogen from solubilization by uranyl acetate.I found that osmium ferrocyanide similarly protected glycogen. My studies showed, however, that the electron density of mitochondria and other cytoplasmic organelles was lower in samples fixed with osmium ferrocyanide than in samples fixed with osmium ferricyanide.

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

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