Effect of potassium orotate on ultrastructure of heart muscle cells in experimental myocardial infarction

1973 ◽  
Vol 75 (4) ◽  
pp. 461-464 ◽  
Author(s):  
N. N. Kleimenova ◽  
S. P. alekseeva ◽  
E. E. Belen'kii
Keyword(s):  
Myocardial Infarction ◽  
Heart Muscle ◽  
Muscle Cells ◽  
Experimental Myocardial Infarction ◽  
Potassium Orotate ◽  
Heart Muscle Cells

Unravelling the ultrastructural details of αT‐catenin‐deficient cell–cell contacts between heart muscle cells by the use of FIB‐SEM

2019 ◽  
Vol 279 (3) ◽  
pp. 189-196 ◽  
Author(s):  
B. VANSLEMBROUCK ◽  
A. KREMER ◽  
F. VAN ROY ◽  
S. LIPPENS ◽  
J. VAN HENGEL
Keyword(s):  
Heart Muscle ◽  
Muscle Cells ◽  
Cell Contacts ◽  
Deficient Cell ◽  
Heart Muscle Cells ◽  
Cell Cell

Effect of pentobarbital on sodium permeability of heart muscle cells

1984 ◽  
Vol 98 (2) ◽  
pp. 1088-1091
Author(s):  
N. V. Dmitrieva ◽  
E. I. Shtresgeim ◽  
N. A. Burnashev ◽  
V. V. Chernokhvostov
Keyword(s):  
Heart Muscle ◽  
Muscle Cells ◽  
Sodium Permeability ◽  
Heart Muscle Cells

Characteristics of active Na transport in intact cardiac cells

1979 ◽  
Vol 236 (2) ◽  
pp. H189-H199 ◽  
Author(s):  
H. G. Glitsch
Keyword(s):  
Heart Muscle ◽  
Muscle Cells ◽  
Cardiac Cells ◽  
Na Transport ◽  
Concentration Gradients ◽  
Experimental Conditions ◽  
Na Pump ◽  
Na Efflux ◽  
Heart Muscle Cells ◽  
K Concentration

An active Na transport maintains the Na and K concentration gradients across the cell membrane of many cells and restores them following excitation. Heart muscle cells display frequent electrical discharges and thus the cardiac Na pump is of fundamental functional significance. Some methods for studying active Na transport are described. The active Na efflux from heart muscle cells is activated by an increase in the intracellular Na and the extracellular K concentration. The linkage between active Na efflux and active K influx varies widely according to the experimental conditions. The cardiac Na pump is electrogenic and can contribute directly to the membrane potential of the cells. The effects of active Na transport on contraction and intercellular coupling in myocardium are discussed.

A confocal laser scanning microscope designed for indicators with ultraviolet excitation wavelengths

1994 ◽  
Vol 266 (1) ◽  
pp. C303-C310 ◽  
Author(s):  
E. Niggli ◽  
D. W. Piston ◽  
M. S. Kirby ◽  
H. Cheng ◽  
D. R. Sandison ◽  
...  
Keyword(s):  
Heart Muscle ◽  
Laser Scanning ◽  
Muscle Cells ◽  
Confocal Laser Scanning Microscope ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Scanning Microscope ◽  
Dual Emission ◽  
Laser Scanning Microscope ◽  
Heart Muscle Cells

In this paper we describe the modifications necessary to upgrade, at affordable cost, a commercially available confocal laser scanning microscope for use with ultraviolet (UV) excitation. The optical problems associated with these modifications are described in detail, and easy solutions to solve them are suggested. The optical resolution of the instrument was tested with fluorescent beads and was found to be close to diffraction limited. The light losses due to lateral chromatic aberration were assessed in a thick fluorescent specimen and were found to be comparable to those usually observed with visible light. For a more visual example of the resolution of this instrument, isolated ventricular heart muscle cells were loaded with the fluorescent Ca2+ indicator indo 1. This allowed us to visualize subcellular structural detail and to illustrate the optical sectioning capability of the UV confocal microscope when recording indo 1 emission. Dual-emission line scans were used to perform ratiometric time-resolved detection of Ca2+ transients in voltage-clamped heart muscle cells loaded with the salt form of indo 1. The system presented in this paper should significantly broaden the range of fluorescent indicators that can be used in confocal microscopy.

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