Total activity and isoenzyme spectrum of lactate dehydrogenase in the normal lens of the human eye and in senile cataract

1972 ◽  
Vol 73 (5) ◽  
pp. 535-537
Author(s):  
B. S. Kasavina ◽  
V. D. Zangieva ◽  
V. V. Drozhzheva
Keyword(s):  
Lactate Dehydrogenase ◽  
Total Activity ◽  
Senile Cataract ◽  
Human Eye ◽  
Normal Lens ◽  
Isoenzyme Spectrum

A search for the best buffer to use in assaying human lactate dehydrogenase with the lactate-to-pyruvate reaction.

1976 ◽  
Vol 22 (11) ◽  
pp. 1872-1875 ◽  
Author(s):  
S N Buhl ◽  
K Y Jackson ◽  
R Lubinski ◽  
R E Vanderlinde
Keyword(s):  
Lactate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Total Activity ◽  
Buffering Capacity ◽  
Buffer Concentration ◽  
Commercial Preparation ◽  
Lactate Dehydrogenases

Abstract Highly purified human lactate dehydrogenases I and V were assayed in 17 different buffers, at a variety of reaction pH's. Diethanolamine and 2-amino-2-methyl-1,3-propanediol provided the best measurements of the enzyme, assayed lactate-to-pyruvate. However, the commercial preparation of 2-amino-2-methyl-1,3-propanediol contained insoluble matter and was relatively expensive. All of the four buffers nowmost commonly used were found to present difficulties. Glycine and pyrophosphate were inhibotory tolactate dehydrogenase activity with increasing buffer concentration. 2-Amino-2-methyl-1-propanol had three major disadvantages: it is chemically unstable during reagent preparation; activity is dependent on buffer concentration; and the pH optima for isoenzymes I and V are vastly different. The pKa of tris(hydroxymethyl)aminomethane is 8.0 at 30 degrees C, whereas to measure total activity the reaction pH should be greater than 8.5; thus tris(hydroxymethyl)aminomethane has limited buffering capacity at the reaction pH.

A new method for reporting the sources of abnormal activities of lactate dehydrogenase in serum.

1986 ◽  
Vol 32 (8) ◽  
pp. 1517-1524 ◽  
Author(s):  
P E Politser ◽  
S H Powell ◽  
J Fink
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Lactate Dehydrogenase ◽  
Total Activity ◽  
New Method ◽  
Additional Insight ◽  
Diagnostic Indices ◽  
Organ Specific ◽  
Diagnostic Usefulness ◽  
Insight Into

Abstract We have developed and tested a new method to increase the diagnostic usefulness of measurements of lactate dehydrogenase (LDH; EC 1.1.1.27) isoenzymes. The method estimates the separate contributions from enzymatically distinct organ clusters (e.g., heart/kidney/erythrocyte, liver/muscle, lung) to the total activity of LDH in serum. To test this method, we monitored serum LDH isoenzymes over the entire hospital course of 73 patients admitted to the intensive-care unit with chest pain, myocardial infarction, or serious hemodynamic disturbances. The organ-specific estimates provided useful information beyond measurements of the original isoenzymes. The sensitivity and specificity of this new method in detecting acute myocardial infarction, as well as concomitant disorders involving the liver or lung, are significantly greater than those of other diagnostic indices or pathologists' judgments. Serial plots of the organ-specific estimates may provide additional insight into evolving pathophysiological processes.

The lactate dehydrogenases of the chicken: estimation and repression during the development of lymphoid and other tissues

1968 ◽  
Vol 46 (6) ◽  
pp. 555-562 ◽  
Author(s):  
G. A. Schultz ◽  
R. F. Ruth
Keyword(s):  
Lactate Dehydrogenase ◽  
Total Activity ◽  
Bursa Of Fabricius ◽  
Ldh Activity ◽  
Great Excess ◽  
Lactate Dehydrogenases

Eight organs of 148 embryos, chicks, and chickens were used for estimations of total lactate dehydrogenase (LDH) activity and its distribution among the five principal isoenzymes, LDH-1 to LDH-5. Heart and liver have the greatest total activities, and brain, bursa of Fabricius, and thymus have the least. During development, total activities may follow similar trends. The heart parallels the liver, kidney and spleen parallel gastrocnemius, and brain and bursa parallel thymus. The distribution of activity among the isoenzymes is independent of the parallels in total activity: the heart acquires a great excess of LDH-1 relative to LDH-5, the gastrocnemius acquires an excess of LDH-5 relative to LDH-1, and the six other organs maintain an excess of LDH-1. The separate activities of LDH-4 and LDH-5 may remain very low, as in brain, bursa, and thymus. The total LDH activity of lymphoid and other organs may be reduced by 19-nortestosterone, an androgen which suppresses lymphopoiesis. The effect of this androgen on the LDH activity of the bursa may be detected before gross effects are obvious.

Aberrant creatine kinase and lactate dehydrogenase response in a myocardial infarct patient.

1984 ◽  
Vol 30 (10) ◽  
pp. 1708-1709
Author(s):  
D L Smalley ◽  
B Womack ◽  
C Handorf ◽  
S Acchiardo
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Normal Values ◽  
Myocardial Infarct ◽  
Total Activity ◽  
Lactate Dehydrogenase Activity ◽  
Creatine Kinase Mb ◽  
Isoenzyme Patterns

Abstract A 65-year-old woman failed to develop increased creatine kinase or lactate dehydrogenase activity after a myocardial infarction. She had no measurable creatine kinase MB isoenzyme and no detectable patterns of normal LD isoenzyme activity. These determinations five months after the infarction showed normal values for total activity and isoenzyme patterns.

Thin-layer agarose electrophoresis of lactate dehydrogenase isoenzymes in serum: a note on the method of reporting and on the lactate dehydrogenase isoenzyme-1/isoenzyme-2 ratio in acute myocardial infarction.

1979 ◽  
Vol 25 (2) ◽  
pp. 209-211 ◽  
Author(s):  
F Y Leung ◽  
A R Henderson
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Lactate Dehydrogenase ◽  
Thin Layer ◽  
Coronary Care Unit ◽  
Total Activity ◽  
Ratio Test ◽  
Dehydrogenase Isoenzyme ◽  
Agarose Electrophoresis ◽  
Coronary Care

Abstract We assessed the clinical efficacy of a thin-layer agarose electrophoresis assay for lactate dehydrogenase isoenzyme estimation in the diagnosis of acute myocardial infarction. From a population of 228 patients admitted to the Coronary Care Unit with suspected acute myocardial infarction, all 101 positive cases (confirmed by clinical presentation and electrocardiographic changes) were correctly identified with lactate dehydrogenase isoenzyme-1 percentage of total activity above the reference range and with a lactate dehydrogenase isoenzyme-1/isoenzyme-2 ratio of 0.76 or above. This ratio was between 0.45 and 0.74 for 250 healthy subjects. No falsely negative, but 12 falsely positive results were obtained from this Coronary Care Unit population, to give a sensitivity of 100% and a specificity of 90.5% for the ratio test.

scholarly journals Infection of sheep with Strongyloides papillosus: The effect of intensity of parasitic infection as well as therapy with albendazole on certain biochemical parameters in the blood of sheep

2015 ◽  
Vol 69 (1-2) ◽  
pp. 41-61
Author(s):  
Blagoje Dimitrijevic ◽  
Slavoljub Jovic ◽  
Milanka Jezdimirovic ◽  
Dragan Bacic ◽  
Mila Savic ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Total Bilirubin ◽  
Parasitic Infection ◽  
Infection Intensity ◽  
Total Activity ◽  
Control Group ◽  
Growth Trend ◽  
Calcium Phosphorus

The objective of this work was to investigate certain biochemical parameters in the blood of sheep in conditions of different intensity of parasitic infection with Strongyloides papillosus, and also after the therapy with albendazole. The research was carried out on the sheep of Wurtemberg breed (n = 30) in which there was determined low, medium and high intensity of parasitic infection with S. papillosus. The control group (n = 10) consisted of the sheep that were negative to the presence of the parasitic infection. The degree and type of changes were monitored by determining the concentration of glucose, total protein, albumin, Albumin/Globulin ratio, AST, urea, total bilirubin, calcium, phosphorus, lactate dehydrogenase (LDH) total activity and isoenzyme distribution LDH1-5. On the basis of the obtained results it was concluded, through isoenzyme distribution of lactate dehydrogenase, that parasitic infection with S. papillosus can cause damage to the liver, heart muscle and lungs, while after the therapy with albendazole, the most affected organ is the liver. The concentration of glucose, total protein and albumin linearly decreased with the increase of intensity of the parasitic infection (p < 0,05), and after the therapy with albendazole, a drop in concentration of these parameters was at a statistically significantly higher level (p< 0,01). AST activity as well as the concentration of urea and total bilirubin grew linearly along with the intensity of the parasitic infection (p < 0,05). After the therapy with ABZ, AST activity and concentration of total bilirubin were at a statistically significantly higher level (p < 0,001), while urea concentration kept the same level as in the case of the parasitic infection. The values of calcium concentration (p > 0,05) as well as phosphorus (p < 0,05) linearly decreased with the parasitic infection intensity growth. Trend towards the decline of the concentration of these macroelements continues even after the therapy with albendazole (p < 0,001). Considering our previous research in the field of oxidative stress, a phenomenon that is fundamentally related to the changes determined in this work, we strongly recommend that besides antihelmintics, preparations with antioxidative properties should be used in antiparasitic protocols as well. <br><font color="red"><b> This article has been retracted. Link to the retraction <u><a href="http://dx.doi.org/10.2298/VETGL190125001E">10.2298/VETGL190125001E</a><u></b></font>

Lactate dehydrogenase in the synovial tissue in rheumatoid arthritis: Total activity and isoenzyme composition

1971 ◽  
Vol 31 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Seppo Lindy ◽  
Jouni Uitto ◽  
Heikki Turto ◽  
Pentti Rokkanen ◽  
Kauko Vainio
Keyword(s):  
Rheumatoid Arthritis ◽  
Lactate Dehydrogenase ◽  
Synovial Tissue ◽  
Total Activity ◽  
Isoenzyme Composition
Sign in / Sign up

Export Citation Format

Share Document