Effect of chronic infection with influenza virus AO/WSN on lifespan of guinea pig embryonic lung cell cultures in vitro

1970 ◽  
Vol 70 (3) ◽  
pp. 1063-1065
Author(s):  
S. D. Vyalushkina ◽  
V. I. Gavrilov
Keyword(s):  
Influenza Virus ◽  
Guinea Pig ◽  
Cell Cultures ◽  
Chronic Infection ◽  
Embryonic Lung ◽  
Lung Cell Cultures

scholarly journals Isolation and initial propagation of guinea pig adenovirus (GPAdV) in Cavia porcellus cell lines

F1000Research ◽  
2020 ◽  
Vol 8 ◽  
pp. 1597
Author(s):  
Adriana E. Kajon ◽  
Xiaoxin Li ◽  
Gabriel Gonzalez ◽  
Susan Core ◽  
Helga Hofmann-Sieber ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Cell Lines ◽  
Cell Cultures ◽  
Sequence Data ◽  
Respiratory Illness ◽  
Epithelial Cell Line ◽  
Cavia Porcellus ◽  
Diagnostic Assays ◽  
Tracheal Epithelial

Background:  The lack of adequate in vitro systems to isolate and propagate guinea pig adenovirus (GPAdV), a prevalent cause of respiratory illness of varaible severity in laboratory guinea pig colonies worldwide, has precluded its formal characterization to allow for the development of comprehensive diagnostic assays, and for the execution of complex pathogenesis and basic virology studies. Methods: Two strains of GPAdV were isolated in guinea pig (Cavia porcellus) cell cultures from frozen archival infected animal tissue originated from colony outbreaks of pneumonia in Australia and the Czech Republic in 1996. Results: Commercially available guinea pig cell lines from colorectal carcinoma (GPC-16), fetal fibroblast (104-C1) and lung fibroblast (JH4 C1), and the tracheal epithelial cell line GPTEC-T developed in this study were able to support viral infection and early propagation. Sufficient viral DNA was recovered from cell cultures to PCR-amplify and obtain sequence data for the complete hexon gene and partial DNA polymerase and penton base genes. Phylogenetic analysis for the three regions of the genome provided strong evidence confirming GPAdV as a unique species in the genus Mastadenovirus. Conclusions: This study demonstrated the feasibility of propagating GPAdV in cultures of immortalized lines of GP cells of a variety of types, thus establishing a critical foundation for the development of a robust culture platform for virus stock production and titration. The generation and analysis of whole GPAdV genome sequences will provide additional data for a comprehensive description of the genetic organization of the viral genome and for a better assessment of genetic diversity between the two isolated strains.

518 Localization and function of MRP1-5 in human lung cell cultures in vitro

2003 ◽  
Vol 144 ◽  
pp. s139
Author(s):  
A.W. Torky ◽  
E. Stehfest ◽  
H. Foth
Keyword(s):  
Cell Cultures ◽  
Human Lung ◽  
And Function ◽  
Lung Cell Cultures

scholarly journals CYTOMETRIC ASSAY OF TOXICITY OF BRUCELLA ANTIGENS FOR SENSITIZED AND NON-SENSITIZED CELLS FROM THE GUINEA PIG

1962 ◽  
Vol 115 (3) ◽  
pp. 613-622 ◽  
Author(s):  
Charles M. Carpenter ◽  
Morimichi Fukuda ◽  
Charles L. Heiskell
Keyword(s):  
Guinea Pig ◽  
Cell Cultures ◽  
Cytotoxic Effect ◽  
Objective Measure ◽  
Toxic Effects

1. A significant cytotoxic effect of trypsinized, sensitized cell cultures was observed when re-exposed to Brucella antigens in vitro. 2. The cytometric assay of the toxic effects of Brucella antigens using trypsinized cells from sensitized animals provides an objective measure of induced cellular hypersensitivity.

scholarly journals Isolation and initial propagation of guinea pig adenovirus (GPAdV) in Cavia porcellus cell lines

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 1597 ◽  
Author(s):  
Adriana E. Kajon ◽  
Xiaoxin Li ◽  
Gabriel Gonzalez ◽  
Susan Core ◽  
Helga Hofmann-Sieber ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Cell Lines ◽  
Cell Cultures ◽  
Sequence Data ◽  
Respiratory Illness ◽  
Epithelial Cell Line ◽  
Cavia Porcellus ◽  
Diagnostic Assays ◽  
Tracheal Epithelial

Background:  The lack of adequate in vitro systems to isolate and propagate guinea pig adenovirus (GPAdV), a prevalent cause of respiratory illness of varaible severity in laboratory guinea pig colonies worldwide, has precluded its formal characterization to allow for the development of comprehensive diagnostic assays, and for the execution of complex pathogenesis and basic virology studies. Methods: Two strains of GPAdV were isolated in guinea pig (Cavia porcellus) cell cultures from frozen archival infected animal tissue originated from colony outbreaks of pneumonia in Australia and the Czech Republic in 1996. Results: Commercially available guinea pig cell lines from colorectal carcinoma (GPC-16), fetal fibroblast (104-C1) and lung fibroblast (JH4 C1), and the tracheal epithelial cell line GPTEC-T developed in this study were able to support viral infection and early propagation. Sufficient viral DNA was recovered from cell cultures to PCR-amplify and obtain sequence data for the complete hexon gene and partial DNA polymerase and penton base genes. Phylogenetic analysis for the three regions of the genome provided strong evidence confirming GPAdV as a unique species in the genus Mastadenovirus. Conclusions: This study demonstrated the feasibility of propagating GPAdV in cultures of immortalized lines of GP cells of a variety of types, thus establishing a critical foundation for the development of a robust culture platform for virus stock production and titration. The generation and analysis of whole GPAdV genome sequences will provide additional data for a comprehensive description of the genetic organization of the viral genome and for a better assessment of genetic diversity between the two isolated strains.

Inhibition of Human and Animal Platelet Adhesiveness to Glass Bead Columns by Adenosine, Dipyridamole, Chlorpromazine and Acetylsalicylic Acid

1976 ◽  
Vol 36 (02) ◽  
pp. 401-410 ◽  
Author(s):  
Buichi Fujttani ◽  
Toshimichi Tsuboi ◽  
Kazuko Takeno ◽  
Kouichi Yoshida ◽  
Masanao Shimizu
Keyword(s):  
Guinea Pig ◽  
Acetylsalicylic Acid ◽  
Guinea Pigs ◽  
Glass Bead ◽  
Animal Species ◽  
Inhibitory Effect ◽  
Rabbit Platelet ◽  
Platelet Adhesiveness

SummaryThe differences among human, rabbit and guinea-pig platelet adhesiveness as for inhibitions by adenosine, dipyridamole, chlorpromazine and acetylsalicylic acid are described, and the influence of measurement conditions on platelet adhesiveness is also reported. Platelet adhesiveness of human and animal species decreased with an increase of heparin concentrations and an increase of flow rate of blood passing through a glass bead column. Human and rabbit platelet adhesiveness was inhibited in vitro by adenosine, dipyridamole and chlorpromazine, but not by acetylsalicylic acid. On the other hand, guinea-pig platelet adhesiveness was inhibited by the four drugs including acetylsalicylic acid. In in vivo study, adenosine, dipyridamole and chlorpromazine inhibited platelet adhesiveness in rabbits and guinea-pigs. Acetylsalicylic acid showed the inhibitory effect in guinea-pigs, but not in rabbits.

INHIBITION OF LIPOLYTIC ACTIVITY OF SYNTHETIC β1–24 AND β1–39 CORTICOTROPHIN BY ANTI-ACTH ANTISERUM

1966 ◽  
Vol 51 (1) ◽  
pp. 88-94 ◽  
Author(s):  
A. Villanueva ◽  
S. J. H. Ashcroft ◽  
J. P. Felber
Keyword(s):  
Guinea Pig ◽  
Lipolytic Activity ◽  
Peptide Hormones ◽  
Fat Pad ◽  
Double Antibody ◽  
Epididymal Fat ◽  
Antibody Complex ◽  
Radio Immunoassay ◽  
Antigen Antibody

ABSTRACT The synthetic ACTH peptides β1–39 and β1–24 stimulated lipolysis as determined by the rat epididymal fat pad in vitro. The stimulating effect of these peptides was diminished by prior incubation of the peptides with antibodies produced by the guinea-pig against ACTH. The stimulating effect of these hormones was also diminished by the double antibody system used in the radio-immunoassay of ACTH and other peptide hormones, in which incubation with antiserum is followed by precipitation of the antigen-antibody complex by rabbit anti-guinea-pig-γ-globulin.

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