The effect of liver tissue homogenate on growth in the liver of tadpoles

1957 ◽  
Vol 43 (4) ◽  
pp. 489-492
Author(s):  
L. K. Ramanova
Keyword(s):  
Liver Tissue ◽  
Tissue Homogenate ◽  
Liver Tissue Homogenate

scholarly journals Effect of Oyster Mushroom (Pleurotus Florida) on Paracetamol Induced Changes of serum bilirubin level and liver tissue protein in Rats

1970 ◽  
Vol 6 (1) ◽  
pp. 10-15
Author(s):  
Afroza Khanam Sumy ◽  
Nasim Jahan ◽  
Nayma Sultana ◽  
SM Ruhul Amin
Keyword(s):  
Liver Tissue ◽  
Protein Concentration ◽  
Total Bilirubin ◽  
Treated Group ◽  
Oyster Mushroom ◽  
Tissue Homogenate ◽  
Control Group ◽  
Serum Total Bilirubin ◽  
Experimental Group ◽  
Liver Tissue Homogenate

Backgroud: Liver is continuously exposed to a variety of toxic agents like drugs and chemicals that may interfere with hepatic function and may cause hepatic damage. Oyster mushroom is excellently edible, nutritious and has got free radical scavenging activity, thereby may be considered as hepatoprotective agent. Objective: To observe the effect of Oyster mushroom on paracetamol induced changes in serum bilirubin and liver tissue protein in rats. Method: This experimental study was carried out in the Department of Physiology, Sir Salimullah Medical College (SSMC), Dhaka from 1st July 2009 to 30th June 2010. A total number of 34 Wistar albino rats, age ranged from 90 to 120 days, weighing between 150 to 210 grams were selected for the study. After acclimatization for 14 days, they were divided into two groups, control group (Group A) and experimental group (Group B- mushroom pretreated and paracetamol treated group). Control group was again subdivided into group A1 (baseline control) and group A2 (paracetamol treated control group). All groups of animals received basal diet for 30 consecutive days. Group A1 consisted of 10 rats, received propylene glycol (2 ml/kg bw, orally) only on 30th day. Group A2 consisted of 14 rats, received single dose of paracetamol suspension (750 mg/ kg bw, orally) only on 30th day. Group B consisted of 10 rats, received mushroom extract (200 mg/ kg bw, orally) for 30 consecutive days and paracetamol suspension (750 mg/ kg bw, orally) only on 30th day. All the animals were sacrificed on 31st day. Then blood and liver sample were collected. Estimation of serum total bilirubin level and assessment of protein concentration in liver tissue homogenate were done by using standard laboratory kits. The statistical analysis was done by one way ANOVA and Bonferroni test as applicable. Result: The mean serum total bilirubin was significantly (p< 0.001) higher in paracetamol treated group in comparison to that of baseline control group. Again, the mean serum total bilirubin was significantly (p<0.001) lower in mushroom pretreated and paracetamol treated group (experimental group) when compared to that of paracetamol treated group (control). The protein concentration in liver tissue homogenate was significantly (p<0.01) lower in paracetamol treated group in comparison to that of baseline control group. Again, in the liver tissue homogenate protein concentration was significantly (p<0.001) higher in mushroom pretreated and paracetamol treated group (experimental group) when compared to that of paracetamol treated group (control). Conclusion: The present study revealed that Oyster mushroom can protect liver tissue against paracetamol induced liver damage. Key words: Hepatoprotective; Oyster mushroom; Tissue homogenate DOI: http://dx.doi.org/10.3329/jbsp.v6i1.8060 J Bangladesh Soc Physiol. 2011 June; 6(1): 10-15

scholarly journals Partial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) Liver

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Hossein Tayefi-Nasrabadi ◽  
Reza Rahmani
Keyword(s):  
Rainbow Trout ◽  
Relative Activity ◽  
Tissue Homogenate ◽  
Partial Purification ◽  
Toxic Substances ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Liver Tissue Homogenate

Cyanide is one of the most toxic substances present in a wide variety of food materials that are consumed by animals. Rhodanese, a ubiquitous enzyme, can catalyse the detoxification of cyanide by sulphuration reaction. In this study, rhodanese was partially purified and characterized from the liver tissue homogenate of the rainbow trout. The enzyme was active in a broad range of pH, from 5 to 12. The optimal activity was found at a high pH (pH 10.5), and the temperature optimum was25∘C. The enzyme was heat labile, losing > 50% of relative activity after only 5 min of incubation at40∘C. TheKmvalues for KCN and Na2S2O3as substrates were 36.81 mM and 19.84 mM, respectively. Studies on the enzyme with a number of cations showed that the activity of the enzyme was not affected by Sn2+, but Hg2+, Ba2+, Pb2+, and Ca2+inhibited and Cu2+activated the enzyme with a concentration-dependent manner.

Effect of cholinotropic agents to change the content of oxygen triplet forms of liver tissue and the ability of the liver homogenate to produce active oxygen in rat cooling

2016 ◽  
Vol 14 (4) ◽  
pp. 3-8
Author(s):  
Viktor I Tikhanov
Keyword(s):  
Liver Tissue ◽  
Liver Homogenate ◽  
Active Oxygen ◽  
Tissue Homogenate ◽  
Pharmacological Agents ◽  
Active Forms Of Oxygen ◽  
Experimental Animals ◽  
Living Organisms ◽  
Content Of Oxygen

The method of polarographic analysis of elements in the tissue solutions of living organisms, and the method of checking the ability of the liver homogenate to produce active forms of oxygen are among the methods for assessing the content of triplet forms of oxygen in the tissue and the ability of the tissue homogenate to initiate lipid peroxidation (POL) after a cold load and administration of cholinotropic agents to rats. In the study, the content of triplet form of oxygen of liver homogenate and the ability of the liver homogenate to produce active oxygen in the period of 3 hour and 5 days cooling of experimental animals were determined. The effect of cholinotropic agent accumulating the endogenous acetylcholine in liver tissue, pharmacological agents that stimulate and block the work of muscarine-sensitive cholinoreactive structures of hepatocyte plasmatic membranes with the assessment of their impact on the content of triplet forms of oxygen, the ability of the liver homogenate to produce active oxygen in supercooling of animals were investigated. Neostigmine on the background of 3 hours cold exposure led to a decrease in the content of triplet forms of oxygen but increased the ability of the liver homogenate to produce active forms of oxygen. Pilocarpine and atropine on the background of cooling animals in 5 days period caused the manifestation of reciprocity as in 3 minutes, so as in 30 minutes of experiment for the determination of triplet forms of oxygen of the liver tissue. Besides pilocarpine and atropine reduced the ability of liver tissue to produce active forms of oxygen. The obtained results indicate the change in the content of triplet forms of oxygen in the liver tissue when pilocarpine and atropine administrated to animals on the backdrop of 5 days cooling. Additionally the results show that the injection of neostigmine to animals on the background of 3 hours cooling promotes the increase of active forms of oxygen.

scholarly journals Antioxidant and Hypolipidemic Potential of Aged Garlic Extract and Its Constituent, S-Allyl Cysteine, in Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Syed Mohammed Basheeruddin Asdaq
Keyword(s):  
Liver Tissue ◽  
Thiobarbituric Acid ◽  
Garlic Extract ◽  
Tissue Homogenate ◽  
Protective Effects ◽  
Aged Garlic Extract ◽  
Active Constituent ◽  
Antioxidant Power ◽  
Potential Implication ◽  
Aged Garlic

Aged garlic extract (AGE) is one of the unique preparations standardized with 100% bioavailable active ingredients found in the bloodstream. The current research was aimed at exploring the role of AGE and its chief active constituent, s-allyl cysteine (SAC) as antioxidant and hypolipidemic agent in rats. At the end of treatment of AGE and SAC, separated serum and freshly prepared liver tissue homogenate were analyzed for biochemical enzymes and biomarkers to evaluate and compare potencies of investigational agents. Both AGE and SAC significantly declined elevated levels of triglyceride, total cholesterol, ALP, AST, ALT, malondialdehyde, glutathione peroxidase enzyme activity, total glutathione and oxidised glutathione in serum and inclined superoxide dismutase, catalase, ferric reducing/antioxidant power, and total sulfhydryl values in liver tissue with reduction in thiobarbituric acid reactive species. The protective effects were superior with AGE compared with SAC indicating potential implication of other active constituents apart from SAC in AGE for combating hyperlipidemic stress.

scholarly journals An h.p.l.c. assay for protoporphyrinogen oxidase activity in rat liver

1987 ◽  
Vol 243 (3) ◽  
pp. 863-866 ◽  
Author(s):  
F Li ◽  
C K Lim ◽  
T J Peters
Keyword(s):  
Rat Liver ◽  
Oxidase Activity ◽  
Mitochondrial Protein ◽  
Protoporphyrin Ix ◽  
Internal Standard ◽  
Reversed Phase ◽  
Tissue Homogenate ◽  
Protoporphyrinogen Oxidase ◽  
Liver Tissue Homogenate

An h.p.l.c. method is described for the assay of protoporphyrinogen oxidase activity in rat liver. A relatively pure protoporphyrinogen IX substrate was obtained by selectively removing any protoporphyrin IX unreduced by sodium amalgam on a small disposable cartridge packed with a strong anion-exchanger. The protoporphyrin IX formed was extracted with dimethyl sulphoxide/methanol (3:7, v/v) containing mesoporphyrin as the internal standard for separation and quantification by reversed-phase chromatography. The Km for protoporphyrinogen was 9.5 +/- 1.6 microM, and the enzyme activities were 0.59 +/- 0.11 nmol of protoporphyrin IX produced/min per mg of mitochondrial protein and 33.5 +/- 2.7 nmol protoporphyrin IX produced/min per g of liver tissue homogenate. The method is applicable to the determination of enzyme activity in small amounts of human liver biopsy.

scholarly journals Human hepatic N-acetylglutamate content and N-acetylglutamate synthase activity. Determination by stable isotope dilution

1990 ◽  
Vol 271 (2) ◽  
pp. 325-329 ◽  
Author(s):  
M Tuchman ◽  
R A Holzknecht
Keyword(s):  
Stable Isotope ◽  
Isotope Dilution ◽  
Liver Tissue ◽  
Internal Standard ◽  
Tissue Homogenate ◽  
Selected Ion Monitoring ◽  
Human Liver Tissue ◽  
Stable Isotope Dilution ◽  
Acetylglutamate Synthase

N-Acetyl-L-glutamate (N-acetylglutamate) content and N-acetylglutamate synthase activity ranges were established in human liver tissue homogenates by stable isotope dilution. The methods employ N-[methyl-2H3]acetyl[15N]glutamate as internal standard, extraction of N-acetylglutamate by anion-exchange technique and its determination by g.l.c.-mass spectrometry by using selected ion monitoring. Hepatic N-acetylglutamate content in 16 different human livers, normal in structure and function, ranged from 6.8 to 59.7 nmol/g wet wt. (25.0 +/- 13.4 mean +/- S.D.) or from 64.6 to 497.6 nmol/g of protein (223.2 +/- 104.2 mean +/- S.D.). In vitro, N-acetylglutamate synthase activity in liver tissue homogenate ranged from 44.5 to 374.5 (132.0 +/- 90.6 mean +/- S.D.) nmol/min per g wet wt. or from 491.7 to 3416.9 (1159.6 +/- 751.1 mean +/- S.D.) nmol/min per g of protein. No correlation was found between hepatic N-acetylglutamate concentrations and the respective maximal enzymic activities in vitro of N-acetylglutamate synthase. The marked variability in this system among individual livers may reflect its regulatory role in ureagenesis.

scholarly journals A BIOCHEMICAL AND HISTOCHEMICAL STUDY OF GLUTAMIC OXALACETIC TRANSAMINASE ACTIVITY OF RAT HEPATIC MITOCHONDRIA FIXED IN SITU AND IN VITRO

1968 ◽  
Vol 39 (3) ◽  
pp. 725-732 ◽  
Author(s):  
Sin Hang Lee ◽  
Richard M. Torack
Keyword(s):  
Histochemical Study ◽  
Intact Cell ◽  
Mitochondrial Fraction ◽  
Tissue Homogenate ◽  
Transaminase Activity ◽  
Glutamic Oxalacetic Transaminase ◽  
Histochemical Methods ◽  
Liver Tissue Homogenate

Rat liver perfused in situ briefly with a glutaraldehyde-formaldehyde mixture was homogenized in isotonic sucrose. The mitochondria, isolated from a homogenate of the perfused liver by differential centrifugation, assumed a slender and compact appearance similar to those often seen in an intact cell. The glutamic oxalacetic transaminase (GOT) activity of this mitochondrial fraction survived an additional formaldehyde fixation and was studied by biochemical and histochemical methods. The biochemical assay of the enzyme activity revealed that the activity was only slightly less than that of an unfixed mitochondrial fraction. The reaction product due to mitochondrial GOT activity was found to be localized to the cristae, as had been demonstrated in an intact liver cell. GOT activity of the mitochondrial fraction isolated from fresh liver tissue homogenate in 0.25 M sucrose was inactivated readily by either glutaraldehyde or formaldehyde and was no longer demonstrable by biochemical and histochemical methods after fixation.

scholarly journals Hepatoprotective effect of Moringa oleifera extract on TNF-α and TGF-β expression in acetaminophen-induced liver fibrosis in rats

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Omnia Aly ◽  
Dalia M. Abouelfadl ◽  
Olfat G. Shaker ◽  
Gehan A. Hegazy ◽  
Ahmed M. Fayez ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Liver Tissue ◽  
Moringa Oleifera ◽  
Hepatoprotective Effect ◽  
Tissue Homogenate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Albino Rats ◽  
Group I ◽  
Tnf Α

Abstract Background It has been reported that Moringa oleifera (MO) has different medicinal properties. The aim of this study was to evaluate the hepatoprotective role of Moringa oleifera extract on acetaminophen-induced liver fibrosis in albino rats on a biochemical and histological basis. Forty male albino rats were divided into four groups: group I (control group), healthy rates; group II (acetaminophen group), rates received acetaminophen for induction of liver fibrosis; group III (treated group), liver fibrosis of rates treated with Moringa oleifera extract; and group IV (prophylactic group), rates treated with Moringa oleifera extract before and after induction of liver fibrosis. Serum liver function parameters were quantified using a spectrophotometer, while tumor necrosis factor α (TNF-α) and transformed growth factor beta (TGF- β) in liver tissue homogenate by means of enzyme-linked immunosorbent assay (ELISA), and expression of liver tissue TNF-α and TGF-genes was measured by real-time PCR after extraction and purification. Hepatic tissue was also evaluated under a microscope for histopathological changes. Results Our results showed a significant decrease in liver enzymes, TNF-α, and TGF-β in the treated and prophylactic groups compared to the acetaminophen group, and our biochemical data were consistent with the histopathological findings confirming the hepatoprotective effect of Moringa oleifera extract. Conclusions Biochemical parameters and histopathology results provide evidence that Moringa oleifera ethanolic extract has a great potential to prevent and improve liver damage due to its protective activity.

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