Autoradiographic analysis of the effect of potassium orotate on the intensity of [5-3H]uridine and [3H]proline into wound fibroblasts

1979 ◽  
Vol 87 (6) ◽  
pp. 653-655
Author(s):  
G. N. Dudnikova
Keyword(s):  
Potassium Orotate ◽  
Autoradiographic Analysis

Incorporation of Cholesterol into Peripheral Nerve Myelin

1972 ◽  
Vol 30 ◽  
pp. 334-335
Author(s):  
Frank A. Rawlins
Keyword(s):  
Electron Microscope ◽  
Sciatic Nerve ◽  
Electron Microscope Autoradiography ◽  
Myelin Sheaths ◽  
Microscope Autoradiography ◽  
Grain Distribution ◽  
Sciatic Nerves ◽  
Autoradiographic Analysis ◽  
Old Mice ◽  
Short Times

Several speculations exist as to the site of incorporation of preformed molecules into myelin. The possibility that an autoradiographic analysis of cholesterol-1,2-H3 incorporation at very short times after injection might shed some light in the solution of that problem led to the present experiment.Cholesterol-1,2-H3 was injected intraperitoneally into 24 tenday old mice. The animals were then sacrificed at 10,20,30,40,60,90,120 and 180 min after the injection and the sciatic nerves were processed for electron microscope autoradiography. To analyze the grain distribution in the autoradiograms of cross and longitudinal sections from each sciatic nerve myelin sheaths were subdivided into three compartments named: outer 1/3, middle 1/3 and inner 1/3 compartments.It was found that twenty min. after the injection of cholesterol -1.2-H3 (Figs. 1 and 2), 55% of the total number of grains (t.n.g) found in myelin were within the outer 1/3 compartment, 9% were within the middle 1/3 and 36% within the inner 1/3 compartment

Autoradiographic analysis of 5-HT2A binding sites in the brain of Sardinian alcohol-preferring and nonpreferring rats

1999 ◽  
Vol 373 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Roberto Ciccocioppo ◽  
Stefania Angeletti ◽  
Giancarlo Colombo ◽  
GianLuigi Gessa ◽  
Maurizio Massi
Keyword(s):  
Binding Sites ◽  
Autoradiographic Analysis ◽  
The Brain

Quantitative receptor autoradiographic analysis for angiotensin II receptors in bovine retinal microvessels: quantitation with radioluminography

1993 ◽  
Vol 13 (3) ◽  
pp. 233-245 ◽  
Author(s):  
Takaya Sato ◽  
Masami Niwa ◽  
Akihiko Himeno ◽  
Keisuke Tsutsumi ◽  
Tsugio Amemiya
Keyword(s):  
Angiotensin Ii ◽  
Angiotensin Ii Receptors ◽  
Autoradiographic Analysis

The disposition of benzo(a)pyrene in the periphyton communities of two South Carolina streams: uptake and biotransformation

1982 ◽  
Vol 60 (10) ◽  
pp. 2084-2091 ◽  
Author(s):  
Mary G. Bruno ◽  
Timothy E. Fannin ◽  
Gordon J. Leversee
Keyword(s):  
South Carolina ◽  
Slide Surface ◽  
Uptake Rates ◽  
Area Basis ◽  
Autoradiographic Analysis ◽  
Colonization Time ◽  
Microscope Slides ◽  
Surface Area Basis ◽  
Glass Microscope ◽  
Sheath Material

The effect of periphyton community composition and colonization time on the uptake and biotransformation of benzo(a)pyrene (BaP) was determined in laboratory studies. Naturally colonized glass microscope slides were collected after 3 and 6 weeks from Castor Creek, which has a predominantly desmid flora, and after 3 and 5 weeks from diatom-dominated Upper Three Runs Creek. When expressed on a slide surface-area basis, the Castor Creek periphyton showed significantly greater BaP uptake rates at both colonization periods. Within streams, uptake rates increased significantly with colonization time. Autoradiographic analysis suggests that BaP was accumulated by surface sorption, especially to gelatinous sheath material. Active biotransformation as measured by the percentage extractable non-BaP 14C was not detected in either community.

scholarly journals THE MEDIATOR OF CELLULAR IMMUNITY

1973 ◽  
Vol 137 (3) ◽  
pp. 660-674 ◽  
Author(s):  
D. D. McGregor ◽  
Pamela S. Logie
Keyword(s):  
Host Reaction ◽  
Proliferating Cells ◽  
Cellular Resistance ◽  
Effector Cells ◽  
Profound Impact ◽  
Antimitotic Activity ◽  
Antimitotic Drug ◽  
Autoradiographic Analysis ◽  
Central Lymph ◽  
Listeria Infection

The antimitotic drug vinblastine (Vbl) has a profound impact upon the specifically sensitized lymphocytes that transfer cellular resistance to Listeria monocytogenes. A 12-h pulse of the drug given to prospective donors during the first week of an immunizing Listeria infection inhibits the delivery of protective lymphocytes to the thoracic duct and their subsequent movement into an inflammatory exudate induced in the peritoneal cavity. The effect of Vbl is clearly related to its antimitotic activity, not to an effect on lymphocytes regardless of their position in the division cycle. This conclusion was drawn from an autoradiographic analysis of cells in the lymph of Vbl-treated rats and from failure of the drug to abrogate a known function of small lymphocytes, namely, their ability to initiate a graft-vs.-host reaction. The results imply that large lymphocytes, the rapidly proliferating cells in central lymph, are the principal effector cells responsible for transmitting resistance to L. monocytogenes and provide a plausible explanation for their rapid turnover and short circulating life-span.

Distribution and characterization of 5-HT1D binding sites in the guinea-pig brain: an autoradiographic analysis

1990 ◽  
Vol 183 (3) ◽  
pp. 1109-1110
Author(s):  
H. Sijbesma ◽  
J.C.H.M. Cornelissen ◽  
E.R. de Kloet ◽  
J. Schipper
Keyword(s):  
Guinea Pig ◽  
Binding Sites ◽  
Pig Brain ◽  
Autoradiographic Analysis ◽  
Guinea Pig Brain

scholarly journals Ciliary protein conservation during development in the ciliated protozoan, Oxytricha.

1987 ◽  
Vol 105 (6) ◽  
pp. 2855-2859 ◽  
Author(s):  
G W Grimes ◽  
R H Gavin
Keyword(s):  
Cell Division ◽  
Daughter Cell ◽  
Basal Bodies ◽  
Ciliated Protozoan ◽  
Subunit Exchange ◽  
Electrophoretic Data ◽  
Autoradiographic Analysis ◽  
Protein Conservation ◽  
The One ◽  
Molecular Conservation

The ciliated protozoan Oxytricha fallax possesses multiple highly localized clusters of basal bodies and cilia, all of which are broken down and rebuilt during prefission morphogenesis-with one major exception. The adoral zone of membranelles (AZM) of the ciliate oral apparatus contains approximately 1,500-2,000 basal bodies and cilia, and it is the only compound ciliary structure that is passed morphologically intact to one daughter cell at each cell division. By labeling all proteins in cells, and then picking the one daughter cell possessing the original labeled AZM, we could then evaluate whether or not the ciliary proteins of the AZM were diluted (i.e., either by degradation to constituent amino acids or by subunit exchange) during cell division. Autoradiographic analysis demonstrated that the label was highly conserved in the AZM (i.e., we saw no evidence of turnover), and electrophoretic data illustrate that at least one of the proteins of the AZM is tubulin. We, therefore, conclude that for at least some of the ciliary and basal body proteins of Oxytricha fallax, AZM morphological conservation is essentially equivalent to molecular conservation.

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