Dynamics of insulin secretion in dogs with alloxan diabetes

1978 ◽  
Vol 85 (3) ◽  
pp. 289-292
Author(s):  
L. N. Dagaeva ◽  
F. S. Belikova ◽  
O. I. Podotykina ◽  
A. V. Makarova
Keyword(s):  
Insulin Secretion ◽  
Alloxan Diabetes ◽  
Dynamics Of Insulin Secretion

Effect of somatostatin-28 on dynamics of insulin secretion in perfused rat pancreas

Diabetes ◽  
1991 ◽  
Vol 40 (9) ◽  
pp. 1163-1169 ◽  
Author(s):  
J. W. Ensinck ◽  
E. C. Laschansky ◽  
R. E. Vogel ◽  
D. A. D'Alessio
Keyword(s):  
Insulin Secretion ◽  
Perfused Rat Pancreas ◽  
Rat Pancreas ◽  
Dynamics Of Insulin Secretion

Diabetes mellitus in pheochromocytoma and paraganglioma: Prevalence, dynamics of insulin secretion / sensitivity and predictors of remission

2020 ◽  
Vol 14 (6) ◽  
pp. 2169-2175
Author(s):  
Saurav Khatiwada ◽  
Shipra Agarwal ◽  
Devasenathipathy Kandasamy ◽  
V.P. Jyotsna ◽  
Rajeev Kumar ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Insulin Secretion ◽  
Dynamics Of Insulin Secretion

Dynamics of Insulin Secretion by the Perfused Rat Pancreas

Endocrinology ◽  
1968 ◽  
Vol 83 (3) ◽  
pp. 572-584 ◽  
Author(s):  
DONALD L. CURRY ◽  
LESLIE L. BENNETT ◽  
GEROLD M. GRODSKY
Keyword(s):  
Insulin Secretion ◽  
Perfused Rat Pancreas ◽  
Rat Pancreas ◽  
Dynamics Of Insulin Secretion

Different dynamics of insulin secretion in the perfused pancreas of mouse and rat

1980 ◽  
Vol 93 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Ove Berglund
Keyword(s):  
Insulin Secretion ◽  
Insulin Release ◽  
Glucose Concentration ◽  
Inbred Mice ◽  
Second Phase ◽  
Perfused Pancreas ◽  
First Response ◽  
Dynamics Of Insulin Secretion ◽  
Rats And Mice ◽  
Secretory Pattern

Abstract. The dynamics of insulin release were studied in the perfused pancreas of rats and mice. Perfusion of the rat pancreas with 20 mm D-glucose resulted in the classical biphasic release of insulin with a rising second phase. However, in normal C57BL/KsJ-mice and noninbred mice, whether fed or starved, the second phase was nearly constant. The secretory dymanics of KsJ-mice were essentially the same, whether the glucose concentration was 30 or 20 mm, whether the medium contained 2.56 or 8 mm Ca2+, and whether or not the medium was supplemented with 5 mm pyruvate, 5 mm glutamate, and 5 mm fumarate. Insulin secretion in these mice was almost totally inhibited by omission of Ca2+, and was markedly enhanced by 3-isobutyl-1-methylxanthine. Insulin release during the constant phase was reversed by lowering the glucose concentration. A second rise of glucose from 3 to 20 mm produced a secretory pattern very similar to the first response. These studies indicate that the dynamics of insulin secretion are somewhat different in rats and mice. Since similar results were obtained with C57BL/KsJ-mice and non-inbred mice, the liability of KsJ-mice to develop β-cell failure when stressed by the mutated db gene is not related to the constancy of the second insulin secretory phase.

INSULIN SECRETION OF THE ISOLATED PERFUSED PANCREAS OF THE CHINESE HAMSTER (CRICETULUS GRISEUS)

1974 ◽  
Vol 76 (2) ◽  
pp. 302-318 ◽  
Author(s):  
E. Gerhards ◽  
M. Rühl
Keyword(s):  
Insulin Secretion ◽  
Chinese Hamster ◽  
Cricetulus Griseus ◽  
Perfused Pancreas ◽  
Short Term ◽  
Isolated Perfused Pancreas ◽  
Perfusion Studies ◽  
Dynamics Of Insulin Secretion ◽  
Linear Manner ◽  
Second Peak

ABSTRACT The insulin secretion pattern of the isolated perfused pancreas of the non-diabetic Chinese hamster after stimulation with glucose and tolbutamide is described. Stimulation with 100–800 mg glucose/100 ml leads to an increasing acute insulin secretion. The initial insulin peak reaches its maximum 2–3 min after the beginning of the stimulation with glucose. The amount of insulin initially secreted from the pancreas of fasted hamsters is larger than that of fed animals. The initial insulin peak is followed by a second short-term insulin release. The maximum of the second peak occurs approximately 10–15 min after the initial stimulation with glucose. With 100 mg glucose/100 ml the insulin secretion proceeds in a practically linear manner for a period of 140 min. With 200 and 400 mg glucose/100 ml the insulin secretion remains linear for approximately 80 min. With 200 mg glucose/100 ml about 10 000 μU insulin are secreted within 120 min, with 400 mg glucose/100 ml approximately 20 000 μU are secreted in the same period of time. The insulin secretion is described following repeated short-term stimulation with 200 mg glucose/100 ml and increasing glucose concentrations up to 500 mg/100 ml for 5 min each. In perfusion studies with 400 mg glucose/100 ml and 8 mg puromycin/100 ml the amount of insulin secreted in 150 min is reduced by about 50%. The insulin secretion stimulated by 100 mg glucose/100 ml was greatly increased by 10 mg tolbutamide/100 ml. The insulin secretion pattern at a glucose concentration of 50 mg/100 ml is shown following repeated stimulation with 10 mg tolbutamide/100 ml alternating with tolbutamide-free resting phases of 10 min. The mechanism of insulin secretion following stimulation with glucose and β-cytotrophic antidiabetics is discussed. The isolated perfused pancreas of the Chinese hamster is suitable for the study of the dynamics of insulin secretion.

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