Proliferation of hematopoietic microenvironment precursors in long-term mouse bone marrow cultures

1984 ◽  
Vol 98 (5) ◽  
pp. 1578-1580
Author(s):  
O. A. Gurevich ◽  
N. I. Drize ◽  
I. L. Chertkov
Keyword(s):  
Bone Marrow ◽  
Mouse Bone Marrow ◽  
Hematopoietic Microenvironment ◽  
Bone Marrow Cultures ◽  
Marrow Cultures

scholarly journals Early pre-B-cell transformation induced by the v-fms oncogene in long-term mouse bone marrow cultures.

1989 ◽  
Vol 9 (9) ◽  
pp. 3973-3981 ◽  
Author(s):  
G V Borzillo ◽  
C J Sherr
Keyword(s):  
Bone Marrow ◽  
B Cells ◽  
Chain Gene ◽  
Mouse Bone Marrow ◽  
Feeder Layers ◽  
Bone Marrow Cultures ◽  
B Lymphoid ◽  
Marrow Cultures

Murine long-term bone marrow cultures that support B-lymphoid-cell development were infected with a helper-free retrovirus containing the v-fms oncogene. Infection of B-lymphoid cultures resulted in the rapid clonal outgrowth of early pre-B cells, which grew to high cell densities on stromal cell feeder layers, expressed v-fms-coded glycoproteins, and underwent immunoglobulin heavy-chain gene rearrangements. Late-passage cultures gave rise to factor-independent variants that proliferated in the absence of feeder layers, developed resistance to hydrocortisone, and became tumorigenic in syngeneic mice. The v-fms oncogene therefore recapitulates known effects of the v-abl and bcr-abl oncogenes on B-lineage cells. The ability of v-fms to induce transformation of early pre-B cells in vitro underscores the capacity of oncogenic mutants of the colony-stimulating factor-1 receptor to function outside the mononuclear phagocyte lineage.

Early pre-B-cell transformation induced by the v-fms oncogene in long-term mouse bone marrow cultures

1989 ◽  
Vol 9 (9) ◽  
pp. 3973-3981
Author(s):  
G V Borzillo ◽  
C J Sherr
Keyword(s):  
Bone Marrow ◽  
B Cells ◽  
Chain Gene ◽  
Mouse Bone Marrow ◽  
Feeder Layers ◽  
Bone Marrow Cultures ◽  
B Lymphoid ◽  
Marrow Cultures

Murine long-term bone marrow cultures that support B-lymphoid-cell development were infected with a helper-free retrovirus containing the v-fms oncogene. Infection of B-lymphoid cultures resulted in the rapid clonal outgrowth of early pre-B cells, which grew to high cell densities on stromal cell feeder layers, expressed v-fms-coded glycoproteins, and underwent immunoglobulin heavy-chain gene rearrangements. Late-passage cultures gave rise to factor-independent variants that proliferated in the absence of feeder layers, developed resistance to hydrocortisone, and became tumorigenic in syngeneic mice. The v-fms oncogene therefore recapitulates known effects of the v-abl and bcr-abl oncogenes on B-lineage cells. The ability of v-fms to induce transformation of early pre-B cells in vitro underscores the capacity of oncogenic mutants of the colony-stimulating factor-1 receptor to function outside the mononuclear phagocyte lineage.

Induction of hematopoietic microenvironment by the extracellular matrix from long-term bone marrow cultures

1991 ◽  
Vol 62 (5) ◽  
pp. 160-164 ◽  
Author(s):  
E. Y. Sadovnikova ◽  
E. I. Deryugina ◽  
N. J. Drize ◽  
J. L. Chertkov
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Hematopoietic Microenvironment ◽  
Bone Marrow Cultures ◽  
Marrow Cultures

scholarly journals Cell types associated with fibronectin in long-term mouse bone marrow cultures.

1982 ◽  
Vol 30 (3) ◽  
pp. 235-244 ◽  
Author(s):  
U Reincke ◽  
P Hsieh ◽  
P Mauch ◽  
S Hellman ◽  
L B Chen
Keyword(s):  
Bone Marrow ◽  
Hematopoietic Cells ◽  
Cell Types ◽  
Mouse Bone Marrow ◽  
Cell Adherence ◽  
Fibronectin Matrix ◽  
Bone Marrow Cultures ◽  
Marrow Cultures

The formation of fibronectin matrix was studied in long-term mouse bone marrow cultures. Stromal and hematopoietic cells were observed in situ under phase contrast optics and quantified according to their staining characteristics on smear preparations. Surface fibronectin was demonstrated by indirect immunofluorescence. While only stromal and no hematopoietic cells participated, various stromal cell types differed in their expression of cell surface fibronectin: Reticulum cells contributed the major portion of fibronectin matrix. Elongated, meshwork-forming histiocytes expressed some surface fibronectin, while the flattened, macrophagic histiocytes remained fibronectin negative. These findings were recapitulated during regeneration of scrape wounds in the adherent layers. Isolated fibronectin matrix did not support hematopoietic cell adherence or maintenance, although it had marked effects on stromal cells.

Recent studies of the hematopoietic microenvironment in long-term bone marrow cultures

1989 ◽  
Vol 8 (3) ◽  
pp. 226-235 ◽  
Author(s):  
Joel S. Greenberger ◽  
T. J. FitzGerald ◽  
Pervin Anklesaria
Keyword(s):  
Bone Marrow ◽  
Hematopoietic Microenvironment ◽  
Bone Marrow Cultures ◽  
Marrow Cultures

Formation of Multinucleated Cells that Respond to Osteotropic Hormones in Long Term Human Bone Marrow Cultures*

Endocrinology ◽  
1987 ◽  
Vol 120 (6) ◽  
pp. 2326-2333 ◽  
Author(s):  
B. R. MACDONALD ◽  
N. TAKAHASHI ◽  
L. M. MCMANUS ◽  
J. HOLAHAN, ◽  
G. R. MUNDY ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Multinucleated Cells ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures ◽  
Marrow Cultures
Sign in / Sign up

Export Citation Format

Share Document