Determination of circulating antibodies and tissue-bound γ-globulin in rabbits with allergic myocarditis by the immunofluorescence method

1969 ◽  
Vol 68 (5) ◽  
pp. 1263-1265
Author(s):  
L. V. Beletskaya ◽  
Yu. S. Ismailova ◽  
T. A. Danilova
Keyword(s):  
Immunofluorescence Method ◽  
Allergic Myocarditis ◽  
Circulating Antibodies

Comparative analysis of immunoassay methods for the determination of circulating antibodies to the phospholipase A2 receptor

2020 ◽  
Vol 24 (6) ◽  
pp. 71-77
Author(s):  
O. V. Galkina ◽  
E. O. Bogdanova ◽  
I. M. Zubina ◽  
E. N. Levykina ◽  
S. V. Lapin ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Phospholipase A ◽  
Circulating Antibodies

scholarly journals Two SARS-CoV-2 IgG Immunoassays Comparison and Time-Course Profile of Antibodies Response

2020 ◽  
Author(s):  
Ruggero Dittadi ◽  
Haleh Afshar ◽  
Paolo Carraro
Keyword(s):  
Immune Response ◽  
Time Course ◽  
Automated Systems ◽  
Igg Antibodies ◽  
Quantitative Correlation ◽  
Circulating Antibodies

The role of the immune response to SARS-CoV-2 infection is not yet well known, in particular about the persistence of circulating antibodies. The aim of the study is to compare the results of two automated systems for the determination of IgG antibodies against SARS CoV-2 and to assess the time course of the IgG response after the onset of symptoms for a period longer than that evaluated to date. IgG were measured in 98 specimens of 55 subjects with COVID-19 (time from the onset of symptoms from 3 to 109 days) using the automated tests "Abbott SARS-COV-2 IgG" and the "MAGLUMI 2019-nCoV IgG". The two methods had a concordance of 91.8%, but the quantitative correlation showed very dispersed results. All the specimens resulted positive after 17 days from the onset of the synptoms. However, the median concentrations of IgG, after a rapid increase up to about 20 days, quickly decrease to about 15% of the maximum for Maglumi. The same samples measured by Architect showed a quite constant trend up to 80 day, and then an only moderate decline. The titer of IgG against SARS-CoV-2 in patients exposed to COVID-19 may significantly and rapidly decrease, with a different time-course depending on the method used for the determination.

Clinical value of the determination of anticardial antibodies in patients with acute myocardial infarction

1982 ◽  
Vol 63 (2) ◽  
pp. 13-16
Author(s):  
N. D. Pozdnyak ◽  
I. V. Efremova
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Prognostic Value ◽  
Myocardial Damage ◽  
Immunofluorescence Method ◽  
Clinical Value ◽  
Recurrent Myocardial Infarction ◽  
Immunological Mechanisms ◽  
Antibody Titers

In 281 patients with acute myocardial infarction, the titer of anticardial antibodies was studied using the indirect immunofluorescence method of Koons. With adequate, immunological reactivity, the height of anticardial-antibody titers reflects the extent of necrotic myocardial damage. Their earliest appearance in blood serum (1-2 days of illness) was observed with repeated and recurrent myocardial infarction. Dissociation between the magnitude of necrotic myocardial damage and a low level of anticardial antibodies indicates a "breakdown" of the protective immunological mechanisms and has an unfavorable prognostic value.

scholarly journals Determination of Neospora caninum and Toxoplasma gondii in aborted bovine foetuses by duplex PCR, immunohistochemistry and immunofluorescence methods

2017 ◽  
Vol 73 (6) ◽  
pp. 346-351 ◽  
Author(s):  
Mustafa Ozkaraca ◽  
Bunyamin Irehan ◽  
Ayse Parmaksiz ◽  
Aysel Itik Ekinci ◽  
Selim Comakli
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Positive Staining ◽  
Duplex Pcr ◽  
Immunofluorescence Method ◽  
Specific Staining

This study was aimed at determining the existence of Neospora caninum and Toxoplasma gondii in aborted bovine foetuses. In this research, 102 bovine foetuses were examined by duplex PCR, immunohistochemistry and immunofluorescence to determine the presence of N. caninum and T. gondii. None of the aborted bovine foetuses were shown to have T. gondii, but N. caninum was detected in 26 foetuses (25.49%) by duplex PCR, in 18 (17.64%) by immunohistochemistry and in 8 (7.84%) by immunofluorescence. Moreover, 16 livers, 13 kidneys, 12 spleens, 8 thymuses and 5 brains of the 18 foetuses examined by immunohistochemistry showed immunopositivity. Positive staining was found in the spleen of eight foetuses by the immunofluorescence method. In this study, immunohistochemistry was shown to be superior to immunofluorescence in terms of diagnosis. Although immunofluorescence has the advantage of being the easiest to use in practice, it makes diagnosis more difficult because of its non-specific staining.

scholarly journals Diagnostics of thrombocytopenias

2018 ◽  
Vol 90 (7) ◽  
pp. 4-13
Author(s):  
A V Mazurov ◽  
S G Khaspekova ◽  
S A Vasiliev
Keyword(s):  
Blood Stream ◽  
Laboratory Diagnostics ◽  
Normal Range ◽  
Laboratory Methods ◽  
Heparin Induced Thrombocytopenia ◽  
Platelet Production ◽  
Reticulated Platelets ◽  
Circulating Antibodies ◽  
Alloimmune Thrombocytopenia

Laboratory methods used for the diagnostics of thrombocytopenias are reviewed. Differential diagnosis is usually carried out between immune and hypoproductive forms of thrombocytopenia. Immune thrombocytopenias are caused by appearance in blood of antiplatelet abtibodies and accelerated destruction of platelets sensibilized by those antibodies, and hypoproductive thrombocytopenias - by impaired platelet production in the bone marrow. Main directions of the laboratory diagnostics of thrombocytopenias - analysis of auto - and alloautoantibodies and evaluation of platelet production and turnover in the blood stream. The following methods are used for the investigation of antiplatelet antibodies: 1) measurement of platelet associated immunoglobulins; 2) determination of circulating antibodies reacting with platelets; 3) determination of antibodies using antigen specific methods - by their reactivity with isolated platelet antigens (glycoproteins). Efficacy of platelet production could be assessed by measuring in blood the amount of “young” (reticulated) platelets. One more method for the evaluation of platelet production as well as the rate of platelet turnover - measurement of plasma soluble glycocalicin, glycoprotein Ib fragment shed from the surface of platelets upon their destruction in spleen and liver. In patients with immune thrombocytopenia autoantibodies are evaluated in all cases, the percentage of reticulated platelets is significantly increased and the amount of plasma glycocalicin is within the normal range or increased. In patients with hypoproductive thrombocytopenia autoantibodies are not detected or detected at low level, the percentage of reticulated platelets is within the normal range or slightly increased and the amount of plasma glycocalicin is lowered. Diagnostics of hapten forms of immune thromocytopenias (heparin-induced thrombocytopenia and others) and of alloimmune thrombocytopenias (neonatal alloimmune thrombocytopenia in particular) are considered in the separate sections of this review.

scholarly journals Immunofluorescence assay of pesticides on the base of immobilized multi-polyclonal antibody

2019 ◽  
Vol 2 (1) ◽  
pp. 46
Author(s):  
Yavor Ivanov ◽  
Marina Yaneva ◽  
Tsonka Godjevargova ◽  
Elena Zvereva
Keyword(s):  
Magnetic Nanoparticles ◽  
Polyclonal Antibody ◽  
Raw Milk ◽  
Immunofluorescence Assay ◽  
Cow Milk ◽  
Immunofluorescence Method ◽  
Buffer Solutions ◽  
Calibration Curves ◽  
Linear Interval

The sensitive competitive immunofluorescence method for simultaneous determination of paraoxon and dichlorvos with immobilized multi-polyclonal antibody on magnetic nanoparticles was developed. The multi-polyclonal antibody was obtained after the immunization of mixture of two prepared immunogens dichlorvos-cBSA and paraoxon-BSA (1:1). The immunogens dichlorvos-cBSA and paraoxon-BSA were synthesized preliminary. Multi-polyclonal antibody against dichlorvos and paraoxon was covalently coupled on magnetic nanoparticles. The competitive fluorescence conjugates dichlorvos-cBSA-FITC and paraoxon-BSA-ATTO 620 were synthesized. Two typical calibration curves of immunofluorescence assay for determination of dichlorvos and paraoxon in buffer solutions were obtained. The linear interval from 2 to 200 ng/mL for these two pesticides was determined. Then the calibration curves for dichlorvos and paraoxon were obtained in cow milk solutions. The linear range of pesticides in cow milk was determined (from 5 to 300 ng/L) and the detection limit for paraoxon (3.5 ng/mL) and dichlorvos (4 ng/mL) was found. The obtained results for cow milk samples were compare with results in UHT, pasteurized cow milk, sheep and goal milk. Quite different are the results when analyzing paraoxon and dichlorvos in standard solutions prepared in sheep's milk. The linear working range for the two pesticides is between 7 and 300 ng/mL it is obviously, that there is a shift of all analytical characteristics up to higher values. The reason for this is the high fat content of sheep's milk. The obtained results were showed that the developed method was 2 time more sensitive than method with the results obtained with mixture (1:1) of two separated antibodies – anti-paraoxon and anti-dichlorvos. These results confirmed the potential of the immunoassay for quantitative simultaneous screening of both dichlorvos and paraoxon. Practical applications:The use of immunofluorescence assay based on immobilized multi-polyclonal antibody on magnetic nanoparticles allows us to detect simultaneous paraoxon and dichlorovos in raw milk and ather diary products.

Further studies on circulating antibodies to liver tissue by immunofluorescence method

1967 ◽  
Vol 2 (2) ◽  
pp. 149-150
Author(s):  
H. Enomoto ◽  
M. Noda ◽  
Y. Nakazawa ◽  
T. Masuda ◽  
Y. Katsuta ◽  
...  
Keyword(s):  
Liver Tissue ◽  
Immunofluorescence Method ◽  
Circulating Antibodies

Comparison between direct and indirect immunofluorescence method for determination of somatic cell count

2018 ◽  
Vol 72 (8) ◽  
pp. 1861-1867 ◽  
Author(s):  
Zlatina R. Becheva ◽  
Katya I. Gabrovska ◽  
Tzonka Ivanova Godjevargova
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Indirect Immunofluorescence ◽  
Somatic Cell Count ◽  
Immunofluorescence Method
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