The effect of sarcolysin on the 24-hour periodicity of mitoses in some albino rat tissues

1964 ◽  
Vol 57 (3) ◽  
pp. 346-349
Author(s):  
V. N. Dobrokhotov ◽  
I. V. Markelova ◽  
L. V. Skolova ◽  
T. B. Timashkevich ◽  
R. I. Nikanorova ◽  
...  
Keyword(s):  
Author(s):  
Ghofran Filaih Abd AL-Hussan ◽  
Waleed Jaleel Abed AL-Kelaby ◽  
Ali Yas Khudhair AL-Ameri

Introduction and Aim: An extract of the lipopeptides from Bacillus subtilis has been found to be extremely useful in antimicrobial applications. Specifically, the goal of this study is to manufacture lipopeptide and assess the safety of this substance on the tissues and organs of laboratory rats. Materials and Methods: Bacillus subtilis bacteria were isolated and identified at the postgraduate microbiology laboratory in the department of biology at Kufa University's College of Science. The bacteria became active after 24 hours of growth in the brain broth infusion broth medium. In order to determine the bacteria's capacity to produce lipopeptide. On nutrient agar medium, a quantity of lipopeptide was synthesized utilizing HCL for sediment, yielding an estimated amount of 1.5 gm during a two-month period, then the product was partially purified and lyophilized using a lyophilizer. For one week, the lipopeptide was administered on albino rat tissues such as the kidney, liver, spleen, and small intestine to determine its safety. Results: The results showed no damage or any changes on tested tissues compared with control treatment and all of the previously mentioned organ tissues were completely intact and this is evidence of the safety of the lipopeptide extract for use. Conclusions: It was discovered that lipopeptide had no effect on the organs that were utilized in the experiment and that it is safe for human consumption.


1972 ◽  
Vol 74 (2) ◽  
pp. 908-910
Author(s):  
E. S. Padalka ◽  
Ya. I. Gonskii ◽  
B. Yu. Samelyuk
Keyword(s):  

2021 ◽  
Author(s):  
NİLGÜN KUŞÇULU

Abstract Background: Apart from the areas where natural dyes are used a lot, if staining studies on tissues increase, their use in pathology laboratory will become widespread. Two basic elements in cell tissues, the nucleus, and cytoplasm, should be stained and evaluated together for histopathological diagnosis. The most commonly used dye combinations for this diagnosis are Hematoxylin-Eosin dyes, also known as nuclei and cytoplasm dyes. Histological staining studies of natural origin did not reveal staining studies on the same tissue with the extracts of two plants. Methods and Results: In this study, roselle (Hibiscus sabdariffa L.) and red cabbage (Brassica oleracea) leaves were used as a source of natural dye and also liver and kidney tissues of Wistar albino rat as dye biomaterials. The nuclei and cytoplasm regions of rat kidney and liver tissues were stained in different colors with these plants and the use of metal salts. Relying upon hematoxylin-eosin should it ever be scarce will result in the scarcity of plant-based dyes on the market.Conclusion: Since a more colorful, different nucleus and cytoplasm staining was obtained compared to previous work with the same tissues. It is expected that the introduction of new natural dye alternatives where histological dye production is not widespread will contribute to the world’s economy and industry.


2007 ◽  
Vol 6 (20) ◽  
pp. 2303-2309 ◽  
Author(s):  
Nagi Reddy P ◽  
Sudhakar Reddy P ◽  
Rajeswara Rao M
Keyword(s):  

Author(s):  
R. Carriere

The external orbital gland of the albino rat exhibits both sexual dimorphism and histological age changes. In males, many cells attain a remarkable degree of polyploidy and an increase of polyploid cell number constitutes the major age change until young adulthood. The acini of young adults have a small lumen and are composed of tall serous cells. Subsequently, many acini acquire a larger lumen with an irregular outline while numerous vacuoles accumulate throughout the secretory cells. At the same time, vesicular acini with a large lumen surrounded by pale-staining low cuboidal diploid cells begin to appear and their number increases throughout old age. The fine structure of external orbital glands from both sexes has been explored and in considering acinar cells from males, emphasis was given to the form of the Golgi membranes and to nuclear infoldings of cytoplasmic constituents.


Sign in / Sign up

Export Citation Format

Share Document