Poly(A)-mRNA deprimerones in rat liver and Novikoff hepatoma cells

1980 ◽  
Vol 6 (2) ◽  
pp. 89-94 ◽  
Author(s):  
M. Hiliar ◽  
J. Przyjemski ◽  
Z. Stolzmann
Keyword(s):  
Rat Liver ◽  
Hepatoma Cells ◽  
Novikoff Hepatoma

DNA-binding peptides from rat liver and Novikoff hepatoma cells: quantitative level and possible biochemical differences

1983 ◽  
Vol 9 (3) ◽  
pp. 169-174 ◽  
Author(s):  
G. L. Gianfranceschi ◽  
D. Barra ◽  
S. Coderoni ◽  
M. Paparelli ◽  
F. Venanzi ◽  
...  
Keyword(s):  
Dna Binding ◽  
Rat Liver ◽  
Hepatoma Cells ◽  
Novikoff Hepatoma ◽  
Quantitative Level ◽  
Binding Peptides

scholarly journals Repression of the albumin gene in Novikoff hepatoma cells.

1982 ◽  
Vol 2 (3) ◽  
pp. 258-266 ◽  
Author(s):  
Y G Capetanaki ◽  
C N Flytzanis ◽  
A Alonso
Keyword(s):  
Rat Liver ◽  
Cdna Probe ◽  
Hepatoma Cells ◽  
In Vitro Translation ◽  
Specific Gene ◽  
Transcriptional Level ◽  
Novikoff Hepatoma ◽  
Albumin Gene ◽  
Albumin Mrna

Novikoff hepatoma cells have lost their capacity to synthesize albumin. As a first approach to study the mechanisms underlying this event, in vitro translation in a reticulocyte system was performed using total polyadenylated mRNA from rat liver and Novikoff hepatoma cells. Immunoprecipitation of the in vitro translation products with albumin-specific antibody revealed a total lack of albumin synthesis in Novikoff hepatoma, suggesting the absence of functional albumin mRNA in these cells. Titration experiments using as probe albumin cDNA cloned in pBR322 plasmid demonstrated the absence of albumin-specific sequences in both polysomal and nuclear polyadenylated and total RNA from Novikoff cells. This albumin recombinant plasmid was obtained by screening a rat liver cDNA library with albumin [32P]cDNA reverse transcribed from immuno-precipitated mRNA. The presence of an albumin-specific gene insert was documented with translation assays as well as by restriction mapping. Repression of the albumin gene at the transcriptional level was further demonstrated by RNA blotting experiments using the cloned albumin cDNA probe. Genomic DNA blots using the cloned albumin cDNA as probe did not reveal any large-scale deletions, insertions, or rearrangements in the albumin gene, suggesting that the processes involved in the suppression of albumin mRNA synthesis do not involve extensive genomic rearrangements.

Repression of the albumin gene in Novikoff hepatoma cells

1982 ◽  
Vol 2 (3) ◽  
pp. 258-266
Author(s):  
Y G Capetanaki ◽  
C N Flytzanis ◽  
A Alonso
Keyword(s):  
Rat Liver ◽  
Cdna Probe ◽  
Hepatoma Cells ◽  
In Vitro Translation ◽  
Specific Gene ◽  
Transcriptional Level ◽  
Novikoff Hepatoma ◽  
Albumin Gene ◽  
Albumin Mrna

Novikoff hepatoma cells have lost their capacity to synthesize albumin. As a first approach to study the mechanisms underlying this event, in vitro translation in a reticulocyte system was performed using total polyadenylated mRNA from rat liver and Novikoff hepatoma cells. Immunoprecipitation of the in vitro translation products with albumin-specific antibody revealed a total lack of albumin synthesis in Novikoff hepatoma, suggesting the absence of functional albumin mRNA in these cells. Titration experiments using as probe albumin cDNA cloned in pBR322 plasmid demonstrated the absence of albumin-specific sequences in both polysomal and nuclear polyadenylated and total RNA from Novikoff cells. This albumin recombinant plasmid was obtained by screening a rat liver cDNA library with albumin [32P]cDNA reverse transcribed from immuno-precipitated mRNA. The presence of an albumin-specific gene insert was documented with translation assays as well as by restriction mapping. Repression of the albumin gene at the transcriptional level was further demonstrated by RNA blotting experiments using the cloned albumin cDNA probe. Genomic DNA blots using the cloned albumin cDNA as probe did not reveal any large-scale deletions, insertions, or rearrangements in the albumin gene, suggesting that the processes involved in the suppression of albumin mRNA synthesis do not involve extensive genomic rearrangements.

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