Characterization of replication-defective transforming RNA tumor viruses by in vitro translation

1979 ◽  
Vol 5 (1-2) ◽  
pp. 115-120
Author(s):  
D. N. Deobagkar ◽  
W. J. M. van de Ven ◽  
A. S. Khan ◽  
F. H. Reynolds ◽  
J. R. Stephenson
Keyword(s):  
In Vitro Translation ◽  
Tumor Viruses ◽  
Rna Tumor Viruses

scholarly journals Characterization of the stimulators of protein-directed ribosomal frameshifting in Theiler's murine encephalomyelitis virus

2019 ◽  
Vol 47 (15) ◽  
pp. 8207-8223 ◽  
Author(s):  
Sawsan Napthine ◽  
Susanne Bell ◽  
Chris H Hill ◽  
Ian Brierley ◽  
Andrew E Firth
Keyword(s):  
Virus Infection ◽  
Rna Structure ◽  
Encephalomyocarditis Virus ◽  
In Vitro Translation ◽  
Theiler's Murine Encephalomyelitis Virus ◽  
Ribosomal Frameshifting ◽  
Theiler’S Murine Encephalomyelitis Virus ◽  
Encephalomyelitis Virus

AbstractMany viruses utilize programmed –1 ribosomal frameshifting (–1 PRF) to express additional proteins or to produce frameshift and non-frameshift protein products at a fixed stoichiometric ratio. PRF is also utilized in the expression of a small number of cellular genes. Frameshifting is typically stimulated by signals contained within the mRNA: a ‘slippery’ sequence and a 3′-adjacent RNA structure. Recently, we showed that −1 PRF in encephalomyocarditis virus (EMCV) is trans-activated by the viral 2A protein, leading to a temporal change in PRF efficiency from 0% to 70% during virus infection. Here we analyzed PRF in the related Theiler's murine encephalomyelitis virus (TMEV). We show that 2A is also required for PRF in TMEV and can stimulate PRF to levels as high as 58% in rabbit reticulocyte cell-free translations and 81% during virus infection. We also show that TMEV 2A trans-activates PRF on the EMCV signal but not vice versa. We present an extensive mutational analysis of the frameshift stimulators (mRNA signals and 2A protein) analysing activity in in vitro translation, electrophoretic mobility shift and in vitro ribosome pausing assays. We also investigate the PRF mRNA signal with RNA structure probing. Our results substantially extend previous characterization of protein-stimulated PRF.

In vitro association of unique species of cellular 4S RNA with 35S RNA of RNA tumor viruses

1974 ◽  
Vol 60 (2) ◽  
pp. 489-497 ◽  
Author(s):  
Larry C. Waters ◽  
Beth C. Mullin ◽  
Ti Ho ◽  
W.K. Yang
Keyword(s):  
Tumor Viruses ◽  
Unique Species ◽  
Rna Tumor Viruses

In vitro translation of human pheochromocytoma messenger RNAs: Characterization of tyrosine-hydroxylase and dopamine-β-hydroxylase

Biochimie ◽  
1985 ◽  
Vol 67 (6) ◽  
pp. 589-595 ◽  
Author(s):  
Caroline Benlot ◽  
Joséphine Antreassian ◽  
Jean-Pierre Henry ◽  
Jean-Claude Legrand ◽  
François Gros ◽  
...  
Keyword(s):  
Tyrosine Hydroxylase ◽  
In Vitro Translation ◽  
Messenger Rnas
Sign in / Sign up

Export Citation Format

Share Document