The relationship between guanosine tetraphosphate, polysomes and RNA synthesis in amino acid starved escherichia coli

1978 ◽  
Vol 4 (1) ◽  
pp. 15-29 ◽  
Author(s):  
P. Donini ◽  
V. Santonastaso ◽  
J. Roche ◽  
A. J. Cozzone
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Rna Synthesis ◽  
Guanosine Tetraphosphate ◽  
The Relationship

scholarly journals Multi-step resistance to Chloramphenicol in RC-stringent Escherichia coli K12—its effect on the induction of RNA synthesis by antibiotics under amino acid starvation

1965 ◽  
Vol 6 (2) ◽  
pp. 304-309 ◽  
Author(s):  
E. C. R. Reeve ◽  
J. O. Bishop
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Resistant Strain ◽  
Parent Strain ◽  
Rna Synthesis ◽  
Amino Acid Starvation ◽  
Sensitive Strain ◽  
Escherichia Coli K12 ◽  
High Doses

A multi-step Chloramphenicol (CM)-resistant derivative of an RC-stringent strain of Escherichia coli auxotrophic for threonine and leucine was resistant also to Aureomycin (AM) and Puromycin (PM). All three antibiotics released the repression of RNA synthesis due to amino acid starvation in the CM-sensitive parent strain, their relative activities being about 1:10:100 for AM: CM: PM. High doses of AM and CM failed to induce RNA synthesis. The CM-resistant strain required greater concentrations of each antibiotic than the sensitive strain to induce the same level of RNA synthesis, and appeared to be about one hundred times, ten times and five times more resistant to CM, AM and PM, respectively, than the sensitive strain.

The relationship between the spoT gene, the synthesis of stable RNA, ribosomal proteins, and the ββ′ subunits of RNA polymerase following a nutritional shiftup of Escherichia coli

1978 ◽  
Vol 56 (6) ◽  
pp. 528-533 ◽  
Author(s):  
Stephen M. Boyle ◽  
Frederick Chu ◽  
Nathan Brot ◽  
Bruce H. Sells
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Ribosomal Protein ◽  
Ribosomal Proteins ◽  
Rna Synthesis ◽  
Transient Increase ◽  
Spot Gene ◽  
The Relationship

The level of ppGpp and rates of synthesis of stable RNA, ribosomal protein, and the β and β′ subunits of RNA polymerase were measured following a nutritional shiftup in Escherichia coli strains, NF 929 (spoT+) and NF 930 (spoT'−). In the spoT+ strain, ppGpp levels decreased 50% within 2 min following shiftup, and the rates of synthesis of stable RNA, ribosomal proteins, and the β and β′ subunits of RNA polymerase increased with little or no lag. In contrast, in the spoT− strain, ppGpp levels transiently increased 40% during the first 6 min following shiftup. An inhibition in the rate of stable RNA synthesis and a delay in the increased synthesis of ribosomal proteins and β and β′ subunits occurred concurrently with the transient increase in ppGpp. In addition, the DNA-dependent synthesis in vitro of the β and β′ subunits of RNA polymerase was inhibited by physiological levels of ppGpp. Because of the timing and magnitude of the changes in ppGpp levels in the spoT− strain versus the timing when the new rates of stable RNA, ribosomal protein, and β and β′ subunits synthesis are reached, it is concluded that ppGpp is not the sole element regulating the expression of these genes.

scholarly journals Synthesis of ribonucleic acid in purine-deficient Escherichia coli and a comparison with the effects of amino acid starvation

1970 ◽  
Vol 120 (1) ◽  
pp. 125-132 ◽  
Author(s):  
N. F. Varney ◽  
Gillian A. Thomas ◽  
K. Burton
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Rna Polymerase ◽  
Ribonucleic Acid ◽  
Rna Synthesis ◽  
Adenine Nucleotides ◽  
Amino Acid Starvation ◽  
Guanine Nucleotides ◽  
Purine Nucleotides ◽  
Acid Control

1. Experiments with rifampicin and stringent strains of Escherichia coli (pro−purB−rel+) indicate that purine deficiency does not decrease and may considerably increase the potential for RNA synthesis by RNA polymerase molecules that are bound to DNA and have already commenced transcription. 2. DNA–RNA hybridization experiments indicate that purine starvation increases the distribution of bound RNA polymerase molecules between the cistrons for mRNA and those for stable RNA. 3. Synthesis of β-galactosidase mRNA is more dependent on the ability to synthesize guanine nucleotides than on the ability to synthesize adenine nucleotides. 4. Amino acid starvation tends to decrease the potential for RNA synthesis by RNA polymerase molecules bound to DNA. 5. Since this effect differs from that due to purine starvation, amino acid control of RNA synthesis does not appear to operate solely by causing a deficiency of purine nucleotides. 6. The results are discussed in terms of the ability to initiate RNA chains and to extend them under different circumstances.

scholarly journals Variations in resistance to three antibiotics among some single-step mutants to Chloramphenicol resistance in a strain of Escherichia coli K 12

1965 ◽  
Vol 6 (2) ◽  
pp. 310-315 ◽  
Author(s):  
E. C. R. Reeve ◽  
J. O. Bishop
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Rna Synthesis ◽  
Single Step ◽  
Chloramphenicol Resistance ◽  
K 12

Chloramphenicol (CM), Aureomycin (AM) and Puromycin (PM) induce RNA synthesis in RC-stringent Escherichia coli starved of a required amino acid. This fact has been used to develop a method for comparing the levels of resistance of single-step CM-r mutants to the three antibiotics. Three levels of resistance to each antibiotic were found among four mutants selected in a single CM-s strain. The mutant with the highest CM resistance has the lowest AM resistance, and vice versa, while the level of PM resistance was not correlated with that of either CM or AM. The four mutants all differed from each other in their patterns of resistance to the three antibiotics.

scholarly journals Amino acid deprivation and its effect on mating ability inEscherichia coliK12

1966 ◽  
Vol 8 (1) ◽  
pp. 115-118 ◽  
Author(s):  
K. W. Fisher
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Deprivation ◽  
Mating Ability ◽  
Chromosome Transfer ◽  
E Coli ◽  
Donor Cells ◽  
Relationship Of ◽  
The Relationship

The conclusion by Suit, Matney, Doudney & Billen (1964) that Hfr donor cells ofEscherichia coliK12, starved of required amino acids can mate, has been re-examined. It appears that their conclusion is not valid and that apparent fertility of amino-acid starved cells is due to cross-feeding by the F−cells. The relationship of this result to the alternative mechanisms for chromosome transfer inE. coliis discussed.

RNA synthesis in T4 infected Escherichia coli during amino acid starvation

Virology ◽  
1972 ◽  
Vol 50 (1) ◽  
pp. 273-276 ◽  
Author(s):  
Pierluigi Donini ◽  
Gordon Edlin
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Rna Synthesis ◽  
Amino Acid Starvation

THE EFFECT OF ACTINOBOLIN ON NUCLEIC ACID AND PROTEIN SYNTHESIS IN ESCHERICHIA COLI

1966 ◽  
Vol 12 (3) ◽  
pp. 515-520 ◽  
Author(s):  
D. E. Hunt ◽  
R. F. Pittillo ◽  
E. P. Johnson ◽  
F. C. Moncrief
Keyword(s):  
Escherichia Coli ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Nucleic Acid ◽  
Mechanism Of Action ◽  
Rna Synthesis ◽  
The Other ◽  
Cross Resistance ◽  
Inhibition Of Protein Synthesis

Actinobolin inhibits protein synthesis in Escherichia coli. When the antibiotic is added to a culture at the time of inoculation, RNA synthesis is also inhibited. Inhibition of RNA synthesis appears to be a consequence of inhibition of protein synthesis. Cross-resistance experiments suggest that the mechanism of action of actinobolin differs from that of the other inhibitors of protein synthesis, chloramphenicol and sparsomycin. Phenylalanine prevents the action of actinobolin provided the amino acid and antibiotic are added simultaneously; this effect is not observed if the phenylalanine is added 1 hour after the addition of the antibiotic. Evidence is presented that the mechanism by which phenylalanine prevents inhibition by actinobolin differs from that which has been suggested for azaserine and p-fluorophenylalanine.

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