Perdeuterioacetylation with combined NMR and MS analysis as a method for determining the evolution of individual hydrolysis products during regioselective enzymatic hydrolysis of glucose pentaacetate

1991 ◽  
Vol 9 (1-2) ◽  
pp. 71-83 ◽  
Author(s):  
David Chaplin ◽  
David H. G. Crout ◽  
David W. Hutchinson ◽  
Oliver W. Howarth ◽  
Riaz Khan
Keyword(s):  
Enzymatic Hydrolysis ◽  
Hydrolysis Products ◽  
Ms Analysis ◽  
Hydrolysis Of ◽  
Glucose Pentaacetate

EFFECTS OF ASCORBIC ACID AND FERUM IONS CONCENTRATION ON THE HYDROLYSIS OF GLUCOSINOLATE AND MYROSINASE ACTIVITY IN THE WATERCRESS (Nasturtium officinale sp.)

2016 ◽  
Vol 78 (8) ◽  
Author(s):  
Syamimi Mohd Zul ◽  
Noumie Surugau
Keyword(s):  
Ascorbic Acid ◽  
Enzymatic Hydrolysis ◽  
Hydrolysis Product ◽  
Carcinogenic Activity ◽  
Nasturtium Officinale ◽  
Hydrolysis Products ◽  
Low Concentrations ◽  
Health Promoting ◽  
Hydrolysis Of ◽  
Myrosinase Activity

Watercress (Nasturtium officinale sp.) from the Brassicae family contains phenethyl glucosinolates (gluconasturtiin) as the main glucosinolate (GLS). The enzymatic hydrolysis products by naturally-occuring myrosinase produced phenethyl isothicyanate (PEITC) which reported to possess anti-carcinogenic activity. Depending on several factors, its counterpart, phenethyl nitrile (PEN) can also be formed as hydrolysis product. This study investigated the effects of adding ascorbic acid and Fe2+ ions at different concentration on the hydrolysis of gluconasturtiin. Hydrolysis products were extracted using dichloromethane and analyzed semi-quantitatively by using GCMS. The results showed that PEITC increased at the low concentration of ascorbic acid (up to 0.06M). Similarly, addition of up to 0.06M Fe2+ ions increased PEITC; higher than 0.06M inhibits the formation of PEITC. Interestingly, similar trend for the production of PEN was detected. This study also investigated myrosinase activity both by exogenous and endogenous methods at different concentrations of ascorbic acid and Fe2+ ions using standard sinigrin as subsrat. Overall, the myrosinase activity was more active at the low concentrations of ascorbic acid. Also, the exogenous method is more efficient than endogenous. This study proved that the presence of reducing agents such as ascorbic acid and Fe2+ ions during the preparation of watercress as food would affect the production of the health-promoting PEITC. 

THE ENZYMATIC HYDROLYSIS PRODUCTS OF SARIN

1956 ◽  
Vol 34 (1) ◽  
pp. 75-79 ◽  
Author(s):  
F. C. G. Hoskin
Keyword(s):  
Enzymatic Hydrolysis ◽  
Cholinesterase Inhibitor ◽  
Methylphosphonic Acid ◽  
Serum Enzyme ◽  
Rat Serum ◽  
Hydrolysis Products ◽  
Hydrolysis Of

The hydrolysis of the powerful cholinesterase inhibitor, sarin, by a rat serum enzyme leads almost exclusively to isopropyl methylphosphonic acid, neither methylphosphonic acid nor hydrogen methylphosphonofluoridate being formed. When isopropyl methylphosphonic acid is administered to the intact rat, it is excreted unchanged in the urine. It is inferred, therefore, that the metabolism of sarin by the intact rat would lead almost exclusively to isopropyl methylphosphonic acid.

scholarly journals Enzymatic Hydrolysis of Marine Collagen and Fibrinogen Proteins in the Presence of Thrombin

Marine Drugs ◽  
2020 ◽  
Vol 18 (4) ◽  
pp. 208 ◽  
Author(s):  
Ludmila L. Semenycheva ◽  
Marfa N. Egorikhina ◽  
Victoria O. Chasova ◽  
Natalya B. Valetova ◽  
Yulia L. Kuznetsova ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Enzymatic Hydrolysis ◽  
Main Part ◽  
Room Temperature ◽  
A Value ◽  
Hydrolysis Products ◽  
Native Collagen ◽  
Before And After ◽  
Molecular Fraction ◽  
Hydrolysis Of

Enzymatic hydrolysis of native collagen and fibrinogen was carried out under comparable conditions at room temperature. The molecular weight parameters of proteins before and after hydrolysis by thrombin were monitored by gel-penetrating chromatography (GPC). An analysis of the experiment results shows that the molecular weight parameters of the initial fibrinogen (Fn) and cod collagen (CC) are very similar. High molecular CC decays within the first minute, forming two low molecular fractions. The main part (~80%) falls on the fraction with a value of Mw less than 10 kDa. The initial high molecular fraction of Fn with Mw ~320–340 kDa is not completely hydrolyzed even after three days of control. The presence of low molecular fractions with Mw ~17 and Mw ~10 kDa in the solution slightly increases within an hour and noticeably increases for three days. The destruction of macromolecules of high molecular collagen to hydrolysis products appears almost completely within the first minute mainly to the polymer with Mw ~10 kDa, and enzymatic hydrolysis of fibrinogen proceeds slower than that of collagen, but also mainly to the polymer with Mw ~10 kDa. Comparative photos of the surfaces of native collagen, fibrinogen and the scaffold based on them were obtained.

scholarly journals Extraction and Identification of Isothiocyanates from Broccolini Seeds

2011 ◽  
Vol 6 (1) ◽  
pp. 1934578X1100600
Author(s):  
Bochao Zhang ◽  
Xiaoqin Wang ◽  
Yanjing Yang ◽  
Xuewu Zhang
Keyword(s):  
Reaction Time ◽  
Brassica Oleracea ◽  
Hydrolysis Products ◽  
Controlled Conditions ◽  
Ms Analysis ◽  
Hydrolysis Of ◽  
Seed Glucosinolates

Broccolini ( Brassica oleracea Italica x Alboglabra) is a cross between broccoli and kai-lan (Chinese broccoli), which contains abundant glucosinolates. The intact glucosinolates are believed to be inactive, while their hydrolysis products, such as isothiocyanates (ITCs), are found to have bacteriocidal and anticarcinogenic activities. So far, no report is available about generation of ITCs during the process of glucosinolate hydrolysis in broccolini. In this study, the hydrolysis of broccolini seed glucosinolates was investigated under controlled conditions of pH, time and temperature, and the ITCs produced were determined. The results showed that an optimum hydrolysis of glucosinolates could be achieved at a temperature of 25°C, at pH 7.0, and a reaction time of eight hours. Furthermore, GC-MS analysis indicated that the extracted ITCs primarily were: 3-BITC (3-benzyl-ITC) (10.8%), 4-methylpentyl-ITC (0.5%), 1-isothiocyanato-butane (26.8%), PEITC (phenethyl-ITC) (22.6%) and SFN (sulforaphane) (19.2%).

scholarly journals Hidrolisis Enzimatis Minyak Kelapa Hidrolisis Enzimatis Minyak Kelapa dengan Lipase dari Rhyzomucor miehei / Enzymatic Hydrolysis of Coconut Oil using Lipase from Rhyzomucor mieheiLipase dari Rhizomucor miehei

Buletin Palma ◽  
2017 ◽  
Vol 17 (1) ◽  
pp. 35
Author(s):  
Steivie Karouw ◽  
Linda Trivana ◽  
Rindengan Barlina ◽  
Budi Santosa
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Enzymatic Hydrolysis ◽  
Silica Gel ◽  
Coconut Oil ◽  
Rhizomucor Miehei ◽  
Enzyme Concentration ◽  
Hydrolysis Reaction ◽  
Hydrolysis Products ◽  
Hydrolysis Of

<p>The objectives  of the research was to find the temperature and time of enzymatic hydrolysis reaction of coconut oil by lipase from Rhizomucor miehei as biocatalyze which could produce the highest free fatty acids.   Enzymatic hydrolysis reaction was held at various of reaction temperature (35, 40, 45 dan 50oC) and reaction duration (6, 12, 18 and 24 hours). Enzymatic hydrolysis reaction was carried out in waterbath  shaker on pH of 7.0 and enzyme concentration of 2.5% based on substrate weight. The hydrolysis products were monitored using TLC using hexane:diethyl eter:acetic acid = 80:20:1 as developing solvent on silica gel F254, 20 cm x 20 cm aluminium plat. The results showed that hydrolyzed coconut oil contained 5 spots, they were identified as 1 spot of triglyceride at upper, 1 spot of free fatty acid, 2 spots of diglyceride at the middle and 1 spot monoglyceride at the bottom. The condition of enzymatic hydrolysis reaction produce the highest of free fatty acid (37.10%) at 50°C of temperature for 24 hours.</p><p>ABSTRAK</p><p><br />Penelitian ini bertujuan untuk mengetahui suhu dan lama reaksi yang dapat menghasilkan asam lemak bebas (ALB) paling banyak pada proses hidrolisis minyak kelapa menggunakan biokatalis lipase dari Rhizomucor miehei. Hidrolisis dilakukan pada variasi suhu reaksi (35, 40, 45 dan 50oC) dan lama reaksi (6, 12, 18 dan 24 jam). Reaksi hidrolisis dilakukan dalam shaker waterbath  dengan pH 7,0 dan kosentrasi enzim 2,5% dari berat substrat. Hasil hidrolisis dianalisis dengan kromatografi lapis tipis (KLT) menggunakan larutan pengembang campuran pelarut heksan:dietil eter:asam asetat = 80:20:1 pada pelat silica gel F254, pelat aluminium 20 cm x 20 cm. Hasil penelitian menunjukkan bahwa hasil hidrolisis minyak kelapa teridentifikasi  lima spot yaitu satu spot trigliserida pada bagian atas, 1 spot asam lemak bebas, dua spot digliserida dan satu spot monogliserida pada bagian bawah. Kondisi hidrolisis minyak kelapa dengan lipase dari R. miehei untuk menghasilkan asam lemak bebas tertinggi yaitu pada suhu 50°C selama 24 jam yaitu sebanyak 37,10%.<br /><br /></p>

THE ENZYMATIC HYDROLYSIS PRODUCTS OF SARIN

1956 ◽  
Vol 34 (1) ◽  
pp. 75-79 ◽  
Author(s):  
F. C. G. Hoskin
Keyword(s):  
Enzymatic Hydrolysis ◽  
Cholinesterase Inhibitor ◽  
Methylphosphonic Acid ◽  
Serum Enzyme ◽  
Rat Serum ◽  
Hydrolysis Products ◽  
Hydrolysis Of

The hydrolysis of the powerful cholinesterase inhibitor, sarin, by a rat serum enzyme leads almost exclusively to isopropyl methylphosphonic acid, neither methylphosphonic acid nor hydrogen methylphosphonofluoridate being formed. When isopropyl methylphosphonic acid is administered to the intact rat, it is excreted unchanged in the urine. It is inferred, therefore, that the metabolism of sarin by the intact rat would lead almost exclusively to isopropyl methylphosphonic acid.

Microwave-assisted enzymatic hydrolysis of starch

2009 ◽  
Author(s):  
Marcin Lukasiewicz ◽  
Anna Osowiec ◽  
Magdalena Marciniak
Keyword(s):  
Enzymatic Hydrolysis ◽  
Microwave Assisted ◽  
Hydrolysis Of

Analyses of enzymatic hydrolysis of bagasse cane of sugar for obtention of ethanol employing a cocktail of natives commercials enzymes.  

2018 ◽  
Author(s):  
Ángel Batallas ◽  
Erenio González ◽  
Carmen Salvador ◽  
Jonathan Villavicencio ◽  
Humberto González Gavilánez ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Hydrolysis Of

Immobilized Nanoparticles-Mediated Enzymatic Hydrolysis of Cellulose for Clean Sugar Production: A Novel Approach

2019 ◽  
Vol 15 (3) ◽  
pp. 296-303 ◽  
Author(s):  
Swapnil Gaikwad ◽  
Avinash P. Ingle ◽  
Silvio Silverio da Silva ◽  
Mahendra Rai
Keyword(s):  
Catalytic Activity ◽  
Enzymatic Hydrolysis ◽  
Immobilized Enzyme ◽  
Free Enzyme ◽  
Magnetic Nanomaterials ◽  
Sugar Production ◽  
Cellulase Enzyme ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.

Sign in / Sign up

Export Citation Format

Share Document