Human ? light chain locus: Organization and DNA sequences of three genomicJ regions

1987 ◽  
Vol 25 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Jenny A. Udey ◽  
Bonnie Blomberg
Keyword(s):  
Light Chain ◽  
Dna Sequences ◽  
Chain Locus ◽  
Light Chain Locus

scholarly journals A complex translocation at the murine kappa light-chain locus.

1987 ◽  
Vol 7 (11) ◽  
pp. 4130-4133 ◽  
Author(s):  
M A Shapiro ◽  
M Weigert
Keyword(s):  
Light Chain ◽  
Chromosome 12 ◽  
Chromosome 15 ◽  
Chromosome 6 ◽  
Kappa Light Chain ◽  
Complex Translocation ◽  
Immunoglobulin Kappa ◽  
Complex Series ◽  
Chain Locus ◽  
Light Chain Locus

We have previously reported that a segment of DNA from a murine plasmacytoma comprises DNA from three chromosomes, the immunoglobulin kappa light-chain locus on chromosome 6, the S mu locus on chromosome 12, and a region on chromosome 15. We now report that the reciprocal product contains DNA from only the kappa locus and chromosome 15 and not from S mu. We conclude that a complex series of events, including both a transposition of DNA and a translocation between chromosomes, generated these imperfect reciprocal products.

Rearrangement of the chicken lambda light chain locus: a silencer/antisilencer regulation

1994 ◽  
Vol 6 (3) ◽  
pp. 165-173 ◽  
Author(s):  
Laurent Ferradini ◽  
Claude-Agnès Reynaud ◽  
Roland Lauster ◽  
Jean-Claude Weill
Keyword(s):  
Light Chain ◽  
Lambda Light Chain ◽  
Chain Locus ◽  
Light Chain Locus

Allelic polymorphisms and RFLP in the human immunoglobulin lambda light chain locus

1999 ◽  
Vol 104 (5) ◽  
pp. 361-369 ◽  
Author(s):  
Marie-Paule Lefranc ◽  
Nathalie Pallarès ◽  
Jean-Pol Frippiat
Keyword(s):  
Light Chain ◽  
Human Immunoglobulin ◽  
Lambda Light Chain ◽  
Chain Locus ◽  
Light Chain Locus ◽  
Immunoglobulin Lambda

Periodicities in nucleotide distribution in the immunoglobulin light chain locus of Gallus gallus

BIOPHYSICS ◽  
2009 ◽  
Vol 54 (4) ◽  
pp. 415-418
Author(s):  
G. I. Kravatskaya ◽  
Yu. V. Kravatsky ◽  
A. S. Blagodatski ◽  
V. G. Tumanyan ◽  
N. G. Esipova
Keyword(s):  
Light Chain ◽  
Gallus Gallus ◽  
Immunoglobulin Light Chain ◽  
Chain Locus ◽  
Light Chain Locus

Expression of v-rel induces mature B-cell lines that reflect the diversity of avian immunoglobulin heavy- and light-chain rearrangements

1988 ◽  
Vol 8 (12) ◽  
pp. 5358-5368
Author(s):  
C F Barth ◽  
E H Humphries
Keyword(s):  
Cell Surface ◽  
B Cell ◽  
Cell Lines ◽  
Light Chain ◽  
Lymphoid Cells ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Chain Locus ◽  
Light Chain Locus

The infection of newly hatched chickens with reticuloendotheliosis virus strain T (REV-T) and a nonimmunosuppressive helper virus, chicken syncytial virus, induces rapidly metastatic B-cell lymphomas. In vivo analysis of these tumors with monoclonal antibodies detected the expression of the B-cell surface markers immunoglobulin M (IgM), CIa, Bu2, and CLA-1, but not IgG, Bu1, or a T-cell surface marker, CT-1. Cell lines derived from tumors exhibited the same pattern of staining, suggesting that expression of cell surface markers does not change during in vitro cell line development. All cell lines examined synthesized IgM in varying amounts. Northern (RNA blot) analysis confirmed abundant expression of v-rel mRNA, and Southern analysis revealed rearrangement of both heavy- and light-chain immunoglobulin loci. Analysis of the light-chain locus demonstrated that 20 of 22 lines contained a single rearranged allele. With respect to specific restriction enzyme sites within the V lambda 1 gene, the active allele in any given clone was either diversified or nondiversified. In contrast, examination of the heavy-chain loci within these lines demonstrated that 16 of the 22 had both alleles rearranged. Further diversification of the V lambda 1 locus did not occur after prolonged in vitro passage of the cell lines. We propose that v-rel expression arrests diversification of the light-chain locus in these lymphoid cells, allowing the production of stable, clonal B-cell populations. The development of these and similar cell lines will make it possible to identify specific stages of avian lymphoid ontogeny and to study the mechanism of rearrangement and diversification in the avian B lymphocyte.

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