Chronic and acute ethanol treatment modifies fluidity and composition in plasma membranes of a human hepaic cell line (WRL-68)

1995 ◽  
Vol 11 (2) ◽  
pp. 69-78 ◽  
Author(s):  
M. C. Guti�rrez-Ruiz ◽  
J. L. G�mez ◽  
V. Souza ◽  
L. Bucio
Keyword(s):  
Cell Line ◽  
Plasma Membranes ◽  
Ethanol Treatment ◽  
Acute Ethanol

Differential gene expression and lipid metabolism in fatty liver induced by acute ethanol treatment in mice

2007 ◽  
Vol 223 (3) ◽  
pp. 225-233 ◽  
Author(s):  
Hu-Quan Yin ◽  
Mingoo Kim ◽  
Ju-Han Kim ◽  
Gu Kong ◽  
Kyung-Sun Kang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lipid Metabolism ◽  
Fatty Liver ◽  
Differential Gene Expression ◽  
Ethanol Treatment ◽  
Acute Ethanol ◽  
Differential Gene

scholarly journals The hyaluronate synthase from a eukaryotic cell line

1993 ◽  
Vol 290 (3) ◽  
pp. 791-795 ◽  
Author(s):  
L Klewes ◽  
E A Turley ◽  
P Prehm
Keyword(s):  
Monoclonal Antibodies ◽  
Phase Separation ◽  
Affinity Chromatography ◽  
Cell Line ◽  
Aqueous Phase ◽  
Plasma Membranes ◽  
Eukaryotic Cell ◽  
Molecular Masses ◽  
Triton X ◽  
Hyaluronate Synthase

The hyaluronate synthase complex was identified in plasma membranes from B6 cells. It contained two subunits of molecular masses 52 kDa and 60 kDa which bound the precursor UDP-GlcA in digitonin solution and partitioned into the aqueous phase, together with nascent hyaluronate upon Triton X-114 phase separation. The 52 kDa protein cross-reacted with poly- and monoclonal antibodies raised against the streptococcal hyaluronate synthase and the 60 kDa protein was recognized by monoclonal antibodies raised against a hyaluronate receptor. The 52 kDa protein was purified to homogeneity by affinity chromatography with monoclonal anti-hyaluronate synthase.

scholarly journals Plasma Membrane Effects of Sphingolipid-Synthesis Inhibition by Myriocin in CHO Cells: A Biophysical and Lipidomic Study

2021 ◽  
Author(s):  
Bingen G. Monasterio ◽  
Noemi Jiménez-Rojo ◽  
Aritz B. García-Arribas ◽  
Howard Riezman ◽  
Félix M. Goñi ◽  
...  
Keyword(s):  
Cell Line ◽  
Cho Cells ◽  
Plasma Membranes ◽  
Mechanical Resistance ◽  
Serine Palmitoyltransferase ◽  
Cho Cell ◽  
Cellular Effects ◽  
Inhibitory Compound ◽  
Membrane Effects ◽  
Biophysical Changes

Abstract Two main strategies for establishing the cellular effects of a given enzyme activity suppression are (a) the use of a stably mutated cell line that lacks a functional gene, or (b) treating the wild type with an inhibitory compound that affects the same gene-product protein. In this work, myriocin was used to block the serine palmitoyltransferase (SPT) enzyme of CHO cells and the subsequent biophysical changes in membranes were measured and compared with results obtained with a genetically modified CHO cell line containing a defective SPT (the LY-B cell line). Similar effects were observed with both approaches: sphingomyelin values were markedly decreased in myriocin-treated CHO cells and, in consequence, their membrane molecular order (measured as laurdan general polarization) and mechanical resistance (AFM-measured breakthrough force values) happened to be lower than in the native, non-treated cells. Cells treated with myriocin reacted homeostatically to maintain membrane order, synthesizing more fully saturated and less polyunsaturated glycerophospholipids than the non-treated ones, although they achieved it only partially, their plasma membranes remaining more fluid and less penetrable than those from the control cells.

Differences in IL-10 and IL-12 production patterns and differences in the effects of acute ethanol treatment on macrophages in vivo and in vitro

Alcohol ◽  
2005 ◽  
Vol 37 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Stephen B. Pruett ◽  
Ruping Fan ◽  
Qiang Zheng ◽  
Carlton Schwab
Keyword(s):  
Ethanol Treatment ◽  
Acute Ethanol

NADPH oxidase contributes to oxidative damage and mitochondrial impairment induced by acute ethanol treatment in rat hippocampal neurons

2020 ◽  
Vol 171 ◽  
pp. 108100 ◽  
Author(s):  
María José Pérez ◽  
Rocío Loyola ◽  
Francisco Canelo ◽  
Alejandra Aranguiz ◽  
Carola Tapia-Monsalves ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Nadph Oxidase ◽  
Hippocampal Neurons ◽  
Ethanol Treatment ◽  
Mitochondrial Impairment ◽  
Acute Ethanol
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