Selection of a sterilization regime for the nutrient medium in lysine biosynthesis

1985 ◽  
Vol 19 (1) ◽  
pp. 71-74
Author(s):  
V. E. Matveev ◽  
V. E. Aksenovskaya ◽  
L. E. Petrosyan ◽  
A. �. Bulutyan ◽  
F. E. Nazaryan ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
Lysine Biosynthesis ◽  
Selection Of ◽  
Sterilization Regime

scholarly journals Development of polyvalent toxoid Clostridium perfringens against anaerobic enterotoxaemia in young cattle and small ruminants

2021 ◽  
Vol 282 ◽  
pp. 04009
Author(s):  
F.Kh. Pulotov ◽  
O.D. Nazarova ◽  
N.A. Akhmadov ◽  
A.I. Karimzoda
Keyword(s):  
Nutrient Medium ◽  
Small Ruminants ◽  
Farm Animals ◽  
Drug Preparation ◽  
Type D ◽  
Industrial Strains ◽  
Type C ◽  
Young Cattle ◽  
C 30 ◽  
Selection Of

The authors of the article presents the results of the development of a polyvalent toxoid from C.perfringens strains against anaerobic enterotoxaemia of young cattle and small ruminants and an assessment of its effectiveness. It is presented the data on the selection of the recipe composition of the nutrient medium, the cultivation of industrial strains, the process of drug preparation, the selection of the optimal ratio of components and the method of controlling the effectiveness. Sequential cultivation of industrial strains of C.perfringens was carried out by cultivating them in flasks, bottles and bioreactors in the nutrient medium developed by the authors, consisting of: 12.0 g/l - liver extract; 25.0 g/l - casein-peptone; 3.2g/l –K2HPO4; 1.8 g/l – KH2PO4; 0.5 g/l – MgSO4. The development of the drug included the selection of optimal and balanced ratios of C. perfringens type A toxoids - 15 IU/ml, C.perfringens type B - 20 IU/ml, C.perfringens type C - 30 IU/ml, C. perfringens type D - 30 IU/ml, which ensured 100% protection of the immunized animals. Studies on laboratory and farm animals revealed the harmlessness, areactogenicity of the drug, which stimulated the development of immunity and resistance to the toxic effects of all strains of C.perfringens.

scholarly journals Development of a probiotic for animals and aquaculture based on Bacillus toyonensis B-13249 and Bacillus pumilus B-13250 strains

2021 ◽  
Vol 11 (3) ◽  
pp. 393-402
Author(s):  
A. V. Malkova ◽  
I. Yu. Evdokimov ◽  
M. V. Shirmanov ◽  
A. N. Irkitova ◽  
D. E. Dudnik
Keyword(s):  
No Value ◽  
Nutrient Medium ◽  
Bacillus Pumilus ◽  
Ph Value ◽  
Expiry Date ◽  
Maximum Value ◽  
Bacillus Genus ◽  
Number Of Bacteria ◽  
Bacillus Toyonensis ◽  
Selection Of

Abstract: This article aims to develop a probiotic for animals and aquaculture based on the Bacillus toyonensis B-13249 and Bacillus pumilus B-13250 strains. The selection of a nutrient medium was conducted for cultivating the inoculum of these microorganisms. Several bacteria fermentations of the Bacillus genus were performed in biological reactors with a capacity of 15 and 250 l. A technology for obtaining a finished probiotic for animals and aquaculture was developed. The results indicate that L-broth is the most optimal nutrient medium for cultivating the studied strains. The cultivation of B. toyonensis B-13249 and B. pumilus B-13250 strains in fermenters revealed that sporulation begins after 4–8 hours of fermentation. In contrast to the vegetative medium, the fermentative medium helped the bacilli develop a higher optical density (the maximum value in the B. pumilus strain – 2.400±0.149), pH value (maximum value in the B. toyonensis strain – 8.483±0.609) and titer (at least 1010 CFU/g). After 20–24 hours of incubation, both strains of bacilli in the fermenter, almost completely pass into endospores, which serve as a signal for the start of biomass centrifugation. This was indicated by the following: from a 15 l fermenter – 83.3±6.1 g of concentrate, from a 250 l fermenter – 499.8±51.4 g. The number of bacilli in a concentrated state was at least 1·1011 CFU/g for both strains. Obtaining a finished preparation required mixing bacterial concentrates with maltodextrin to a titer of at least 1·1010 CFU/g. The number of bacteria in the preparation checked every month during the year, recorded no value less than 1·1010 CFU/g. Thus, L-broth is most favorable for growing the mother culture of the B. toyonensis B-13249 and B. pumilus B-13250 strains, and fermentative nutrient medium – for the cultivation in fermenters. The expiry date of the bacilli-based biological preparation is at least 12 months, during which the drug’s polycomponence, color and consistency are preserved, in addition to the bacteria titer (at least 1·1010 CFU/g) and their viability.

scholarly journals Analysis and selection of an effective method for growing meristemic potato plants (SolanumTuberosum L.)

2021 ◽  
Vol 7 (Special) ◽  
pp. 14-14
Author(s):  
Boris Zherebtsov ◽  
◽  
Alexander Kozlov ◽  
Sergey Netesov
Keyword(s):  
Nutrient Medium ◽  
Traditional Methods ◽  
Potato Plants ◽  
Cultivation Methods ◽  
Selection Of

In this article, methods of growing meristemic plants are considered and the basics of hydroponics techniques are studied. Studies of the main characteristics of growing potato plants in a nutrient medium on the basis of a designed automated hydroponic installation are presented and the indicators are compared with traditional methods. Keywords: HYDROPONICS, GROUNDLESS CULTIVATION METHODS, HYDROPONIC AGRICULTURE, HYDROPONIC TECHNOLOGIES

scholarly journals INTRODUCTION OF EXPLANTS AND REPRODUCTION ON NUTRIENT MEDIUM OF DONOR MATERIAL IN VITRO VARIETIES OF CALLISTEPHUS CHINENSIS (L.) NESS. FOR ITS FURTHER USE IN LANDSCAPING

2020 ◽  
Vol 1 (383) ◽  
pp. 89-96
Author(s):  
V. Polishchuk ◽  
S. Turchina ◽  
A. Balabak ◽  
I. Kozachenko ◽  
V. Mamchur ◽  
...  
Keyword(s):  
Plant Breeding ◽  
Vegetative Propagation ◽  
Nutrient Medium ◽  
Cell Biology ◽  
Plant Biotechnology ◽  
Forest Steppe ◽  
Physical Parameters ◽  
Adaptation Period ◽  
Selection Of

The relevance of the research topic. On the recent methods of biotechnology are increasingly used in plant breeding and seed production. Herbaceous plants such as strawberries, potatoes, a vegetable, some medicinal and others are capable of vegetative propagation the traditional methods of culture, successfully introduced in both in vitro and can achieve a high rate of reproduction. Modern plant biotechnology – the sum of the technologies developed in molecular and cell biology of plants – a new stage in the development of the technology of plant breeding. With these improved characteristics may occur at the level of individual genes and individual genes that determine a specific trait, can be identified. They may be the final selection, they can be isolated, insert, delete, or modify the genotype or variety. Goal. Identify the features of the manifestation of economically valuable features and decorative properties of Callistephus chinensis and the inclusion of the best varieties in the biotech link, their adaptation to the conditions of the Forest-Steppe of Ukraine and their further use in landscaping. Methods. Laboratory – determination of seed germination; mathematical and statistical - for processing the reliability of the obtained research results. Results. The nutrient medium for growing plant tissues and cells, by analogy with the medium for culturing animal tissues, should contain all that the tissues in the plant organism receive from xylem and phloem currents of substances. However, in practice it has been found that vegetable juices cannot serve as a complete nutrient medium for growing isolated tissues and cells. This manifests the specificity of the receipt, transportation and especially the redistribution of nutrients in the plant. Based on the analysis, research was conducted to study the possibility of mass off-season vegetative propagation of plants of Callistephus chinensis in vitro. Practical recommendations on the selection of sterilizer, sterilization, nutrient medium and for the adaptation period of the best genotypes of this culture have been developed. As a result of the conducted researches the methods of selection of the initial plant material of Callistephus chinensis (Callistephus Chinensis (L.) NEES) and its surface sterilization, modification of existing aseptic culture methods have been studied and mastered. The morphogenetic potential of explants from different plant organs was investigated and selection of nutrient medium and study of the influence of plant growth regulators and physical parameters on the process of morphogenesis was carried out. The features of regeneration of isolated explants depending on the composition of the nutrient medium and selection of conditions for obtaining self-clones of Callistephus chinensis (Callistephus Chinensis (L.) NEES) were studied. Key words: in vitro, plant biotechnology, Callistephus Chinensis, nutrient medium, rhizogenesis.

scholarly journals Modeling of asymmetric division of somatic cell in protoplasts culture of higher plants

2020 ◽  
Vol 11 (2) ◽  
pp. 255-265
Author(s):  
S. I. Kondratenko ◽  
T. P. Pasternak ◽  
O. P. Samovol ◽  
O. M. Mogilna ◽  
O. V. Sergienko
Keyword(s):  
Cell Division ◽  
Osmotic Stress ◽  
Growth Regulators ◽  
Nutrient Medium ◽  
Higher Plants ◽  
Asymmetric Division ◽  
Cycle Phase ◽  
In Planta ◽  
Selection Of

The key result of the work is the selection of factors for the cultivation of protoplasts of higher plants in vitro, which allowed induction of asymmetrical cell division during the first cell cycle phase. Gibberellin has been proved to be one of the main cofactors of asymmetric division of plant cells. The objects of research were plants of the following cultivars aseptically grown in hormone-free MS medium: tobacco (Nicotiana tabacum L.), SR-1 line; Arabidopsis thaliana var. columbia (L.) Heynh; potato (Solanum tuberosum L.), Zarevo cultivar; cultivated white head cabbage (Brassica oleraceae var. capitata L.) of the following varieties: Kharkivska zymnia, Ukrainska osin, Yaroslavna, Lika, Lesya, Bilosnizhka, Dithmarscher Früher, Iyunskarannya; rape (Brassica napus L.) of Shpat cultivar; winter radish (Raphanus sativus L.) of Odessa-5 cultivar. In experiments with mesophilic and hypocotyl protoplasts of the above-mentioned plant species it has been proved that short-term osmotic stress within 16–18 hours being combined with subsequent introduction of high doses of gibberellin GK3 (1 mg/L) into the modified liquid nutrient media TM and SW led to the occurrence of pronounced morphological traits of cytodifferentiation already at the initial stages of the development of mitotically active cells in a number of higher plants. Meanwhile, in all analyzed species, there was observed the division of the initial genetically homogeneous population of mitotically active cells into two types of asymmetric division: by the type of division of the mother cell into smaller daughter cells and by the type of the first asymmetric division of the zygotic embryo in planta. In this case, the first type of asymmetric division occurred during unusual cytomorphism of the mother cells: a pronounced heart-shaped form even before the first division, which is inherent in the morphology of somatic plant embryo in vitro at the heart-shaped stage. A particular study of the effect of osmotic stress influencing protoplasts of various cultivars of white cabbage, isolated from hypocotyls of 7–9 day etiolated seedlings, revealed quite a typical consistent pattern: the acquisition and maintenance of the axis of symmetry in growing microcolonies occurred without extra exogenous gibberellin (GK3), which was added to the nutrient medium earlier. While analyzing the effect of growth regulators on the formation of microcolonies with traits of structural organization, the conclusion was made regarding the commonality of the revealed morphogenetic reactions of cells within the culture of protoplasts of higher plants in vitro with similar reactions studied earlier on other plants, both in vitro and in planta. Modeling of asymmetric cell division in protoplast culture in vitro has become possible by carrying out a balanced selection of growth regulators as well as their coordinated application through time along with changes in osmotic pressure of a nutrient medium.

scholarly journals Selection of Optimal Conditions for Cultivation of Bacillus megaterium UCM B-5710 – Producer of Keratinase

2021 ◽  
Vol 83 (6) ◽  
pp. 32-40
Author(s):  
K.V. Avdiyuk ◽  
◽  
A.O. Roy ◽  
Keyword(s):  
Soybean Meal ◽  
Nutrient Medium ◽  
Ph Value ◽  
Soluble Starch ◽  
Enzyme Synthesis ◽  
Cultivation Conditions ◽  
Additional Source ◽  
Carbon And Nitrogen ◽  
Initial Ph ◽  
Selection Of

Every year the volume of production of poultry products all over the world is growing steadily. This contributes to a constant increase in the amount of by-products of poultry processing in the form of down and feather waste, which are dangerous for the environment due to the hard-to-degrade keratin protein and a large number of microbial pathogens. Therefore, the use of environmentally friendly methods for the destruction of keratin substrates due to keratinases of microorganisms is an urgent area of research. The aim of this work was to select the optimal cultivation conditions for the Bacillus megaterium strain UCM B-5710 to increase the activity of the keratinase synthesized by it. Methods. The culture was grown at 28°C, 201 rpm for 7 days on a basic nutrient medium containing defatted chicken feathers as the only source of carbon and nitrogen. The selection of optimal cultivation conditions was carried out according to the following parameters: temperature (21°C, 28°C, 42°C), stirring speed (201 rpm, 212 rpm), amount of inoculum (5%, 10%, 15% , 20%, 25%), the initial pH value of the nutrient medium (4.0–11.0), concentration of keratin-containing substrate (0.1%, 0.2%, 0.5%, 1.0%, 1.5%, 2.0%), additional carbon source (glucose, galactose, lactose, maltose, sucrose, mannitol, potato and corn starch, soluble starch, soybean meal) and nitrogen (NH4Cl, NH4NO3, (NH4)2SO4, NaNO3, urea, peptone, tryptone, yeast extract and soybean meal) at a concentration of 1%. Keratinase activity was assessed by the UV absorption at 280 nm of the hydrolysis products of keratin-containing raw materials. Protein was determined by the Lowry method. Results. The dynamics of the enzyme synthesis showed that the culture of B. megaterium UCM B-5710 exhibited the highest keratinase activity on the 3rd day, and complete splitting of feathers was observed on the 4–5th days. The selection of the concentration of the keratin-containing substrate showed that 0.5% is the optimal concentration. The study of the influence of the initial pH value of the nutrient medium indicates that the culture grew well at pH 6.0–7.0 and pH 9.0–11.0, but at pH 8.0 its growth was very weak. The culture exhibited the maximum keratinase activity at pH 10.0. In addition, at this pH value, complete splitting of feathers was visually observed. The influence of such a key factor as temperature on the growth and synthesis of the enzyme by B. megaterium UCM B-5710 culture demonstrated complete splitting of feathers already on the 2nd day of cultivation at 42°C, at 21°C the culture split feathers very poorly. The introduction of the inoculum into the composition of the nutrient medium in an amount of 15% of the volume of the medium and the mixing intensity of 212 rpm turned out to be optimal. Besides, it was shown that the introduction of an additional source of carbon or nitrogen had an ambiguous effect on the level of keratinase activity of B. megaterium UCM B-5710. Complete inhibition of enzyme synthesis was observed when ammonium sulfate was added to the nutrient medium, and partial inhibition was observed in the case of glucose, lactose, and maltose. Potato, corn, and soluble starch stimulated keratinase synthesis. The majority of inorganic nitrogen sources (ammonium chloride and nitrate) did not affect the synthesis of B. megaterium UCM B-5710 keratinase, while organic sources (urea, peptone, tryptone, yeast extract) increased the level of keratinase activity by 20–50%. However, the most effective result was obtained using soybean meal, the addition of which to the nutrient medium increased the keratinase activity by 2.5 times. Conclusions. As a result of the studies, the optimal conditions for cultivation of the B. megaterium UCM B-5710 strain were selected: the optimum temperature for the growth and development of the culture is 42°C, the initial pH value is 10.0, the stirring speed is 212 rpm and the amount of inoculum introduced is 15%, an additional source of carbon and nitrogen in the form of soybean meal at a concentration of 0.5%. This made it possible to increase the activity of keratinase by 4 times.

scholarly journals Establishment of glyphosate selective concentrations for winter rapeseed (Brassica napus L.) transgenic tissues efficient selection in vitro

2019 ◽  
Vol 25 ◽  
pp. 197-201
Author(s):  
I. S. Hnatiuk ◽  
O. I. Varchenko ◽  
M. F. Parii ◽  
Yu. V. Symonenko
Keyword(s):  
Brassica Napus ◽  
Nutrient Medium ◽  
In Vitro Selection ◽  
Brassica Napus L ◽  
Regeneration Stage ◽  
Winter Rapeseed ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Efficient Selection ◽  
Selection Of

Aim. To establish the optimal concentration of glyphosate for efficient selection of transgenic tissue culture in biotechnology of rapeseed by in vitro selection of glyphosate-resistant and non-resistant winter rape. Methods. As an explants 7–10 mm fragments of 6-day rapeseed hypocotyls were used to cultivate on the medium, supplemented with 1 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D) for 12 days at 24°C under dark conditions for the initiation of callusogenesis. For regeneration of plants, the MS nutrient medium was supplemented with 3 mg/L of 6-benzylaminopurine (BAP), 2 mg/L of zeatin, 0.1, 0.5 and 1 mM of glyphosate, respectively. As a control, glyphosate-free regenerative nutrient medium was used. The results were statistically processed using Microsoft Excel. Results. It has been shown that glyphosate-susceptible rape explants form leaves and shoots with a frequency up to 8.3 % ± 0.8 % on the medium supplemented with 0.1 mM glyphosate compared to 57.2 % ± 0.6 % in the control. However, the morphogenic structures did not pass selection after transferring shoots to the elongation medium supplemented with 0.1 mM glyphosate. Conclusions. For the in vitro selection of rapeseed 0.1 mM glyphosate can be used in a nutrient medium at the stage of adventitious buds formation. But at the regeneration stage, the amount of herbicide should be increased to avoid false-positive results. Keywords: winter rapeseed, Brassica napus L., in vitro culture, selective marker, herbicide, glyphosate.

scholarly journals SELECTION OF OPTIMAL NUTRIENT MEDIUM FOR COLLAGENASE BIOSYNTHESIS BY ASSOCIATION ASPERGILLUS AWAMORI 16 AND ASPERGILLUS AWAMORI 22

2019 ◽  
Vol 1 (331) ◽  
pp. 26-31
Author(s):  
R. K. Blieva ◽  
◽  
Zh. B. Suleimenova ◽  
А. S. Zhakipbekova ◽  
A. K. Kalieva ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
Aspergillus Awamori ◽  
Selection Of

scholarly journals Multi-Step Cellular and Tissue Selection of Soybean for Resistance to Osmotic Stress Using Peg 6000 Invitro Conditions

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Mohammad Naim Noori
Keyword(s):  
Polyethylene Glycol ◽  
Osmotic Stress ◽  
Nutrient Medium ◽  
Selective Agent ◽  
Soybean Variety ◽  
Peg 6000 ◽  
Final Volume ◽  
Polyethylene Glycol 6000 ◽  
Selection Of

Cell and tissue selection in vitro allows targeted selection of the desired traits under severe selective conditions at the level of individual cells and tissues. On the basis of multistage cell and tissue selection with the use of a selective agent - neutral osmotic polyethylene glycol 6000 in increasing concentrations -5%, 10%, 15%, 20% of the final volume of the nutrient medium, callus of the Zhansaya soybean variety that are stably resistant to osmotic stress have been obtained. Regeneration from callus was noted only in8.3 % of the planted callus 6 soybean regenerants plants resistant to osmotic stress were obtained from callus.

scholarly journals Preparatory selection of sterilization regime for canned "Natural Atlantic Mackerel with Oil" based on the developed mathematical models of the process

Vestnik MGTU ◽  
2016 ◽  
Vol 19 (4) ◽  
pp. 861-868
Author(s):  
A. A. Maslov ◽  
◽  
A. V. Stolyanov ◽  
A. V. Kaychenov ◽  
L. K. Kuranova ◽  
...  
Keyword(s):  
Mathematical Models ◽  
Atlantic Mackerel ◽  
Selection Of ◽  
Sterilization Regime
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